Exogenous sodium diethyldithiocarbamate, a Jasmonic acid biosynthesis inhibitor, induced resistance to powdery mildew in wheat

Abstract

Jasmonic acid (JA) is an important plant hormone associated with plant–pathogen defense. To study the role of JA in plant–fungal interactions, we applied a JA biosynthesis inhibitor, sodium diethyldithiocarbamate (DIECA), on wheat leaves. Our results showed that application of 10 mM DIECA 0–2 days before inoculation effectively induced resistance to powdery mildew (Bgt) in wheat. Transcriptome analysis identified 364 up‐regulated and 68 down‐regulated differentially expressed genes (DEGs) in DIECA‐treated leaves compared with water‐treated leaves. Gene ontology (GO) enrichment analysis of the DEGs revealed important GO terms and pathways, in particular, response to growth hormones, activity of glutathione metabolism (e.g., glutathione transferase activity), oxalate oxidase, and chitinase activity. Gene annotaion revealed that some pathogenesis‐related (PR) genes, such as PR1.1, PR1, PR10, PR4a, Chitinase 8, beta‐1,3‐glucanase, RPM1, RGA2, and HSP70, were induced by DIECA treatment. DIECA reduced JA and auxin (IAA) levels, while increased brassinosteroid, glutathione, and ROS lesions in wheat leaves, which corroborated with the transcriptional changes. Our results suggest that DIECA can be applied to increase plant immunity and reduce the severity of Bgt disease in wheat fields.

Whole Genome Sequence of an Edible Mushroom Stropharia rugosoannulata (Daqiugaigu)

“…The higher abundance of proteins involved in glutathione metabolism, as well as glyoxylate and dicarboxylate metabolism, were also observed in the stipe of H. marmoreus compared with the mycelia and cap (in another reviewed article in JOF). Furthermore, the upregulation of these proteins was also reported in wheat when resistance to powdery mildew was induced by sodium diethyldithiocarbamate [62] and in rhizobacteria promoted rice root [63]. Therefore, further studies are needed to analyze the functions of stress response proteins in the elongation of the fruiting body stipe.…”

Secondary Metabolites in Plant Defence Mechanisms

“…On one hand, tomato plants will be exogenous treated with sodium diethyldithiocarbamate (DIECA), that inhibits JA pathway by shunting 13(S)hydroperoxylinolenic acid to 13-hydroxylinolenic [206]. Application of DIECA has been shown to significantly reduce JA levels in multiple plant species and reduce the expression of some resistance gene [207][208][209]. On the other hand, tomato plants that ½General Discussion 138 constitutively over-emit GLVs, will be generated by knocking out the tomato AOS2 gene using CRISPR-Cas9 gene-editing technology.…”