Exogenous inorganic ions, partial dehydration, and high rewarming temperatures improve peach palm (Bactris gasipaes Kunth) embryogenic cluster post-vitrification regrowth

Ree, Joseph Francis; Guerra, Miguel Pedro

doi:10.1007/s11240-020-01852-z

Cited by 8 publications

(9 citation statements)

References 58 publications

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“…In effect, CaMg CW (6.5) significantly reduced EC levels in P50 lettuce seeds ( Figure 1 A) indicating that the inorganic salt hydration treatment had a restorative effect on lettuce seed membranes and reduced the heightened leakage of ions traditionally associated with ageing in seeds. Inorganic ions have been suggested to be involved in protecting cytoskeleton or cell membrane from injury [ 58 ] due to their promotive effect on the structure and stability of membrane lipid bilayer [ 58 ]. In addition, cation-enhanced interactions between lipids allow for closeness between lipid molecules thereby increasing membrane density [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

“…Inorganic ions have been suggested to be involved in protecting cytoskeleton or cell membrane from injury [ 58 ] due to their promotive effect on the structure and stability of membrane lipid bilayer [ 58 ]. In addition, cation-enhanced interactions between lipids allow for closeness between lipid molecules thereby increasing membrane density [ 58 ]. In previous studies, Abdolahi et al [ 31 ] reported that KH 2 PO 4 solution lessened EC in three cultivars (RGS, ‘Hyola 401′ and ‘Pacific’) of Brassica napus seeds subjected to accelerated ageing for different durations (48 and 96 h).…”

Section: Discussionmentioning

confidence: 99%

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Effects of Inorganic Salt Solutions on Vigour, Viability, Oxidative Metabolism and Germination Enzymes in Aged Cabbage and Lettuce Seeds

Adetunji

Sershen

Varghese

et al. 2020

Plants

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This study assessed the potential of pre-hydration treatment with aqueous solutions (electrolysed [cathodic water; CW] and non-electrolysed) prepared from four different inorganic ion combinations: 1 mM CaCl2, 1 µm CaCl2 and 1 mM MgCl2 (CaMg, hereafter), 1 mM MgCl2 and 1 mM NaCl to invigorate controlled deteriorated (CDd) Brassicaoleracea (cabbage) and Lactucasativa (lettuce) seeds by assessing germination, vigour and biochemical markers (electrolyte leakage, lipid peroxidation products, protein carbonylation, and defence and germination associated enzymes) of oxidative stress. Additionally, the possible effects of pH of electrolysed CaMg and NaCl solutions were assessed. The inorganic salt solutions were applied to fresh seeds and seeds deteriorated to 75% viability (P75), 50% viability (P50) and 25% viability (P25); deionised water served as control. The pre-hydration treatment did not enhance normal seedling production in cabbage. However, Ca-containing and CW hydration treatments (CaCl2 CW, CaMg and CaMg CW [6.5], MgCl2 CW, NaCl CW and NaCl CW [6.5]) promoted normal seedling production of CDd lettuce seeds, while seedling vigour was enhanced by CaMg, CaMg CW (6.5), NaCl CW and NaCl CW (6.5) in CDd cabbage seeds, and CaCl2, CaCl2 CW, CaMg, CaMg CW (6.5), MgCl2 CW, NaCl CW and NaCl CW (6.5) in CDd lettuce seeds. The supplementation of Ca, a component of the ionised solutes, and/or the reducing potential of CW contributed to increased normal seedling production in lettuce seeds irrespective of the pH of treatment solutions or degree of deterioration. Overall, the pre-hydration treatments enhanced endogenous antioxidants leading to reduced levels of electrolyte leakage, lipid peroxidation, protein carbonylation, and enhanced germination enzyme activities in lettuce seeds. The study concluded that pre-hydration with selected inorganic salt solutions can invigorate debilitated lettuce seeds.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effects of Inorganic Salt Solutions on Vigour, Viability, Oxidative Metabolism and Germination Enzymes in Aged Cabbage and Lettuce Seeds

Adetunji

Sershen

Varghese

et al. 2020

Plants

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“…tTCL 0.4-0.5 mm [9] Malus spp. tTCL 0.1-0.3 mm [16,17] Jatropha curcas L. tTCL 0.8-1.0 mm [18] Passiflora edulis Sims tTCL 0.5 mm thick [20] Camellia sinensis L. lTCL 0.5 mm [26] Elaeis guineensis × Elaeis oleifera tTCL 1 mm thick [2] Guadua chacoensis (Rojas) Londoño and P.M. tTCL 0.5-1.0 mm [34] Actinidia chinensis tTCL 0.5-1.0 mm thick [35] Bactris gasipaes Kunth tTCL 0.3 mm [36] Bactris gasipaes Kunth tTCL 7.0-9.0 mm [37]…”

Section: Angiospermaementioning

confidence: 99%

Thin Cell Layer Tissue Culture Technology with Emphasis on Tree Species

Sharma,

Magotra,

Chourasia

et al. 2023

Forests

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An increased dependency on plant-based resources for food, shelter, and medicinal usage has increased their sustainable and unsustainable exploitation. To use this resource sustainably, plant tissue culture (PTC) is one important technology. Among different PTC techniques, thin cell layer (TCL) technology is a relatively simple and easily adaptable technique for in vitro cultures of plants. This technique uses small explants about 0.5–2 mm in thickness excised from different plant organs. It has been successfully used in the large-scale propagation of vegetables, legumes, and plants with medicinal benefits. TCL technology has proven to be effective in stimulating various organogenic responses when combined with various new methods such as nanotechnology or microtome-based explantation, especially in tree species. It is considered an important tool in plant biotechnology. Although the morphogenetic response per explant is usually higher in conventional explants, the appropriate use of plant growth regulators and geometric factors in TCL has the potential to make it more efficient and beneficial. This article provides an overview of the concept of TCL as applied to different plant species, particularly trees, since there are few, if any, summaries of TCL technology, especially in trees. This review will certainly revitalize this important technology so that it can be used effectively for successful mass propagation in the field of plant tissue culture.

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“…The PVS3 constraint can be considered rather high viscosity, which can make PVS3 application difficult [57]. On the other hand, the diluted PVS3 solutions, with lower viscosity, in a range from 80 to 100% have acceptable vitrification ability (Figure 2) and have been successfully used as CPAs [42,43,53,54,[58][59][60].…”

Section: Pvs3-based Solutionsmentioning

confidence: 99%

Vitrification Ability of Combined and Single Cryoprotective Agents

Faltus

Bilavčík

Zámečnı́k

2021

Plants

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Cryoprotective agents (CPA) are an important part of many current vitrification methods. The vitrification ability of CPAs influences the probability of the glass transition and water crystallization occurrence. Thermal characteristics and the vitrification ability of two combined CPAs (PVS2 and PVS3), common plant vitrification solutions, and four single CPAs (ethylene glycol, DMSO, glycerol, and sucrose), the components of the mentioned PVSs, were evaluated utilizing a differential scanning calorimetry (DSC) during standard cooling/warming rates of 10 °C min−1. The effect of solute concentration on their vitrification ability was shown in the CPAs tested. Four typical concentration regions at which the glassy state and/or crystallization occurred were defined. We suggest the solute concentration of 0.7 g g−1 as the universal vitrification concentration, characterized by an actual Tg of CPA solution and limited water crystallization. Knowledge of the thermal properties of CPAs allows the design of new combined CPAs with the required vitrification ability respecting the cryopreservation method used and the characteristics of the cryopreserved sample.

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Exogenous inorganic ions, partial dehydration, and high rewarming temperatures improve peach palm (Bactris gasipaes Kunth) embryogenic cluster post-vitrification regrowth

Cited by 8 publications

References 58 publications

Effects of Inorganic Salt Solutions on Vigour, Viability, Oxidative Metabolism and Germination Enzymes in Aged Cabbage and Lettuce Seeds

Effects of Inorganic Salt Solutions on Vigour, Viability, Oxidative Metabolism and Germination Enzymes in Aged Cabbage and Lettuce Seeds

Thin Cell Layer Tissue Culture Technology with Emphasis on Tree Species

Vitrification Ability of Combined and Single Cryoprotective Agents

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