Exogenous glutathione supplementation in culture medium improves the bovine embryo development after in vitro fertilization

Sun, Weijun; Pang, Yuxin; Yan, Liying; Huang, Hao; Zhao, Xiao‐Fan; Tong, Qin; Zhu, Huabin; Du, Weihua

doi:10.1016/j.theriogenology.2015.05.001

Cited by 24 publications

(29 citation statements)

References 41 publications

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“…Supplements in maturation media may affect blastocyst production without affecting the total embryo cell count [28], as observed in our study. Similarly, the ICM/TCN ratios obtained here are in agreement with those reported by Sun et al [29] for bovine blastocyst using the same staining protocol. With this regard, SNP treatments are not deleterious for embryo quality.…”

Section: Treatmentsupporting

confidence: 92%

Effects of short-term exposure of mature oocytes to sodium nitroprusside on in vitro embryo production and gene expression in bovine

et al. 2015

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Section: Treatmentsupporting

confidence: 92%

Effects of short-term exposure of mature oocytes to sodium nitroprusside on in vitro embryo production and gene expression in bovine

et al. 2015

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“…48-168 hpi at 38.5°C in an atmosphere containing 5% CO 2 and 100% humidity (Sun et al, 2015). Cleavage was recorded at 48 hpi.…”

Section: Production Of Bovine Embryos By Ivfmentioning

confidence: 99%

Exogenous glutathione improves intracellular glutathione synthesis via the γ‐glutamyl cycle in bovine zygotes and cleavage embryos

Feng

Cui

et al. 2018

Journal Cellular Physiology

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Excess reactive oxygen species (ROS) generated in embryos during in vitro culture damage cellular macromolecules and embryo development. Glutathione (GSH) scavenges ROS and optimizes the culture system. However, how exogenous GSH influences intracellular GSH and improves the embryo developmental rate is poorly understood. In this study, GSH or GSX (a stable GSH isotope) was added to the culture media of bovine in vitro fertilization embryos for 7 days. The cleavage rate, blastocyst rate, and total cell number of blastocysts were calculated. Similarly to GSH, GSX increased the in vitro development rate and embryo quality. We measured intracellular ROS, GSX, and GSH for 0-32-hr postinsemination (hpi) in embryos (including zygotes at G1, S, and G2 phases and cleaved embryos) cultured in medium containing GSX. Intracellular ROS significantly decreased with increasing intracellular GSH in S-stage zygotes (18 hpi) and cleaved embryos (32 hpi).γ-Glutamyltranspeptidase (GGT) and glutathione synthetase (GSS) messenger RNA expression increased in zygotes (18 hpi) and cleaved embryos treated with GSH, consistent with the tendency of overall GSH content. GGT activity increased significantly in 18 hpi zygotes.GGT and GCL enzyme inhibition with acivicin and buthionine sulfoximine, respectively, decreased cleavage rate, blastocyst rate, total cell number, and GSH and GSX content. All results indicated that exogenous GSH affects intracellular GSH levels through the γglutamyl cycle and improves early embryo development, enhancing our understanding of the redox regulation effects and transport of GSH during embryo culture in vitro.

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“…This molecule has been shown to act by stimulating embryonic development and the synthesis of glutathione (GSH), a compound naturally present in both gametes with an important role in protecting against the effects of ROS (GOTTARDI et al, 2012). Cysteine and glutathione have also been used in IVEP protocols with good results (ALI et al, 2003;SUN et al, 2015). Recently, the quercetin (2 μM), resveratrol (2 μM), vitamin C (50 μg/mL), carnitine (0.5 mg/mL), and cysteamine (100 μM) were evaluated to identify the most efficient antioxidant against the harmful effects of ROS during IVM of bovine oocytes (SOVERNIGO et al, 2017).…”

Section: Use Of Antioxidants In Ivep Mediamentioning

confidence: 99%

Use of natural antioxidants in in vitro mammalian embryo production

Santos

Borges

Neta

et al. 2018

SCA

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In vitro embryo production (IVEP) contributes to the quantitative and qualitative aspects of animal reproduction. Nevertheless, inherent technical factors such as oxidative stress can negatively influence the result and this can impair cell metabolism, thus decreasing the rates of in vitro development, and necessitating the supplementation of culture medium with antioxidants. In this context, compounds of natural origin with this property have been highlighted because of the positive results obtained at different stages of IVEP. Thus, this review aims to present the results obtained by using natural antioxidants to minimize the effects of oxidative stress on gametes and embryos. A variety of natural isolated substances and mixtures (essential oils and extracts) have been studied for supplementation of IVEP media, at stages of in vitro maturation, sperm capacitation, in vitro fertilization, and in vitro development of embryos in different mammalian species. Generally, beneficial effects are observed according to the concentration used, thus demonstrating the potential of several natural antioxidants. Therefore, the main challenges in using these compounds as antioxidants during IVEP include proving their efficiency against free radicals and determining the best concentration at each stage. In addition, understanding the mechanisms of action of such antioxidants is crucial to establishing their use in IVEP biotechnology. Key words: Culture medium. Natural bioactive. Oxidative stress. Reproduction. ResumoA produção in vitro de embriões (PIVE) contribui para os aspectos quantitativos e qualitativos da reprodução animal. Contudo, fatores inerentes da técnica, como o estresse oxidativo, podem influenciar negativamente o resultado e isso pode prejudicar o metabolismo celular, diminuindo assim as taxas de desenvolvimento in vitro, e exigindo a suplementação do meio de cultivo com antioxidantes. Neste contexto, os compostos de origem natural com essa propriedade têm se destacado devido aos resultados positivos obtidos em diferentes estágios da PIVE. Assim, esta revisão pretende apresentar os resultados obtidos usando antioxidantes naturais para minimizar os efeitos do estresse oxidativo em gametas e embriões. Uma variedade de substâncias isoladas e de misturas naturais (óleos essenciais e extratos) tem sido estudada para a suplementação de meios de PIVE, nas etapas de maturação in vitro, capacitação espermática, fecundação in vitro e desenvolvimento in vitro de embriões em diferentes espécies de mamíferos. Geralmente, os efeitos benéficos são observados de acordo com a concentração utilizada,

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Exogenous glutathione supplementation in culture medium improves the bovine embryo development after in vitro fertilization

Cited by 24 publications

References 41 publications

Effects of short-term exposure of mature oocytes to sodium nitroprusside on in vitro embryo production and gene expression in bovine

Effects of short-term exposure of mature oocytes to sodium nitroprusside on in vitro embryo production and gene expression in bovine

Exogenous glutathione improves intracellular glutathione synthesis via the γ‐glutamyl cycle in bovine zygotes and cleavage embryos

Use of natural antioxidants in in vitro mammalian embryo production

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