Excretory–secretory proteome of larval Schistosoma mansoni and Echinostoma caproni, two parasites of Biomphalaria glabrata

Guillou, F.; Roger, Emmanuel; Moné, Yves; Rognon, Anne; Grunau, Christoph; Thèron, André; Mitta, Guillaume; Coustau, Christine; Gourbal, Benjamin

doi:10.1016/j.molbiopara.2007.05.009

Cited by 132 publications

(131 citation statements)

References 87 publications

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“…HSP70s, 14-3-3 proteins, thioredoxin peroxidase-1 (TPX1), EF1␣, malate dehydrogenase, and cathepsin L were also over-represented at a somewhat lesser level. This finding strengthens the notion that parasites use a limited and conserved set of ES proteins for evasion from host immune attacks or for modulation of host responses (34). Also this observation illustrates the importance of these abundant proteins as vaccine candidates and drug targets for control of schistosomiasis and other helminthiasis.…”

Section: Fig 1 Extraction Of Es Proteins Of Adult Worms Of S Japonsupporting

confidence: 82%

“…Importantly this information can be expected to facilitate the discovery of vaccines and new therapeutic drug targets as well as new diagnostic reagents for schistosomiasis control. Proteomics approaches encompass the most efficient and powerful tools for identification of protein complexes and have been widely used to decipher the ES components of the filarial parasite Brugia malayi (19), Leishmania (Trypanosomatidae) (20), nematodes (21)(22)(23)(24)(25)(26), and Trematoda (27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37). For the genus Schistosoma, the ES compositions of S. mansoni have been identified in many developmental life stages, including sporocyst (34), cercaria (35,36), and egg (37) but have not been characterized in the adult worm.…”

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confidence: 99%

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Excretory/Secretory Proteome of the Adult Developmental Stage of Human Blood Fluke, Schistosoma japonicum

Liu

Cui

et al. 2009

Molecular & Cellular Proteomics

165

139

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Schistosomes are the causative agents of schistosomiasis, one of the most prevalent and serious of the parasitic diseases that currently infects ϳ200 million people worldwide. Schistosome excretory/secretory (ES) proteins have been shown to play important roles in modulating mammalian host immune systems. In our current study, we performed a global proteomics identification of the ES proteins from adult worms of Schistosoma japonicum, one of the three major schistosome species. Our results unambiguously identified 101 proteins, including 53 putatively secreted proteins. By quantitative analysis, we revealed fatty acid-binding protein as a major constituent of the in vitro ES proteome. Strikingly the heat shock proteins HSP70s, HSP90, and HSP97 constituted the largest protein family in the ES proteome, implying a central role for these proteins in immunomodulation in the hostparasite relationship. Other important S. japonicum ES proteins included actins, 14-3-3, aminopeptidase, enolase, and glyceraldehyde-3-phosphate dehydrogenase, some of which have been considered as viable vaccine candidates and therapeutic targets. A comparison with previous studies suggests that 48.5% of S. japonicum ES proteins are common to other parasite ES products, indicating that the molecular mechanisms involved in evading the host immune response may be conserved across different parasites. Interestingly seven host proteins, including antimicrobial protein CAP18, immunoglobulins, and a complement component, were identified among in vitro S. japonicum ES products likely originating from the schistosome tegument or gut, indicating that host innate and acquired immune systems could defend against schistosome invasion. Our present study represents the first attempt at profiling S. japonicum ES proteins, provides an insight into host-parasite interactions, and establishes a resource for the devel-

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Section: Fig 1 Extraction Of Es Proteins Of Adult Worms Of S Japonsupporting

confidence: 82%

mentioning

confidence: 99%

Excretory/Secretory Proteome of the Adult Developmental Stage of Human Blood Fluke, Schistosoma japonicum

Liu

Cui

et al. 2009

Molecular & Cellular Proteomics

165

139

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“…This concept of molecular mimicry was introduced by Yoshino and Bayne (1983) when they found haemolymph-like antigens on the surface tegument of sporocysts. Similar mimicry has been suggested in schistosomula by Guillou et al (2007) and Horta and Ramalho-Pinto (1991) by demonstrating that decay-accelerating factor from human erythrocytes could be transferred to S. mansoni and protect it against complement-mediated haemolysis. We observed that the secondary sporocysts were recognised at a lower rate than the primary sporocysts in the in vitro experiments.…”

Section: Discussionsupporting

confidence: 71%

Interaction between primary and secondary sporocysts of Schistosoma mansoni and the internal defence system of Biomphalaria resistant and susceptible to the parasite

Mattos

Martins-Souza

Kusel

et al. 2011

Mem. Inst. Oswaldo Cruz

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“…In addition, these products are also in direct contact with the host, being likely candidates for vaccines. S. mansoni larval secretions have also been the subject of a proteomic study [54], but are of less interest in the scope of this review, since the larvae exist only within the mollusc intermediate host, and a possible treatment of molluscs in the open field to eliminate parasites at this stage would be difficult to implement.…”

Section: Schistosome Secretionsmentioning

confidence: 99%

Schistosomes—proteomics studies for potential novel vaccines and drug targets

DeMarco

Verjovski-Almeida

2009

Drug Discovery Today

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Schistosomiasis is a major health problem and, despite decades of research, only one effective drug, Praziquantel is currently available. Recent expansion of sequence databases on Schistosoma mansoni and S. japonicum has permitted a wealth of novel proteomic studies on several aspects of the organization and development of the parasite in the human host. This unprecedented accumulation of molecular data is allowing a more rational approach to propose drug targets and vaccine candidates, such as proteins located at the parasite surface. Successful preliminary trials of two vaccine candidates that have been detected at the parasite surface by proteomics give grounds for believing that such an approach may provide a fresh start for the field.

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Excretory–secretory proteome of larval Schistosoma mansoni and Echinostoma caproni, two parasites of Biomphalaria glabrata

Cited by 132 publications

References 87 publications

Excretory/Secretory Proteome of the Adult Developmental Stage of Human Blood Fluke, Schistosoma japonicum

Excretory/Secretory Proteome of the Adult Developmental Stage of Human Blood Fluke, Schistosoma japonicum

Interaction between primary and secondary sporocysts of Schistosoma mansoni and the internal defence system of Biomphalaria resistant and susceptible to the parasite

Schistosomes—proteomics studies for potential novel vaccines and drug targets

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