Excreted/secreted Schistosoma mansoni venom allergen-like 9 (SmVAL9) modulates host extracellular matrix remodelling gene expression

Yoshino, Timothy P.; Brown, Martha; Wu, Xiao-Jun; Jackson, C.J.; Ocadiz-Ruiz, Ramon; Chalmers, Iain W.; Kolb, Marlen; Hokke, Cornelis H.; Hoffmann, Karl F.

doi:10.1016/j.ijpara.2014.04.002

Cited by 40 publications

(41 citation statements)

References 72 publications

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“…Whole parasite preparations of S. mansoni, particularly the egg antigens, are known to induce Th2 responses . We sought to validate our protocol by examining immune responses to crude parasite preparations of AWA and SEA.…”

Section: Resultsmentioning

confidence: 99%

“…For rSmTSP6 and rSmTSP7, 84 and 78 amino acids were expressed, representing the extracellular loop 2 of these proteins, respectively, as defined by TMHMM2.0 software . About 106‐189 amino acid of rSmTSP6 and 108‐185 amino acid of rSmTSP7 were expressed using the same vector, expression parameters and purification methods as rSmLy6A and B . Endotoxin contamination of the recombinant antigens was determined by Limulus amebocyte lysate (LAL) assay using the Pierce LAL chromogenic endotoxin quantitation kit (Thermo Fisher, Pittsburgh, PA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Whole parasite preparations of S. mansoni, particularly the egg antigens, are known to induce Th2 responses. [27][28][29] We sought to validate our protocol by examining immune responses to crude parasite preparations of AWA and SEA. As expected, and confirming our analysis approach, the adult worm and soluble egg antigens induced the production of significant levels of IL-5 and IL-13 compared to medium ( Figure 1).…”

Section: Schistosoma Mansoni Adult Worm and Egg Antigens Induce Th2mentioning

confidence: 99%

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Schistosoma mansoni schistosomula antigens induce Th1/Pro‐inflammatory cytokine responses

Egesa

Lubyayi

Tukahebwa

et al. 2018

Parasite Immunology

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Summary Larvae of Schistosoma (schistosomula) are highly susceptible to host immune responses and are attractive prophylactic vaccine targets, although cellular immune responses against schistosomula antigens in endemic human populations are not well characterized. We collected blood and stool from 54 Schistosoma mansoni ‐infected Ugandans, isolated peripheral blood mononuclear cells and stimulated them for 24 hours with schistosome adult worm and soluble egg antigens ( AWA and SEA ), along with schistosomula recombinant proteins rSmKK7, Lymphocyte Antigen 6 isoforms (rSmLy6A and rSmLy6B), tetraspanin isoforms (rSmTSP6 and rSmTSP7). Cytokines, chemokines and growth factors were measured in the culture supernatants using a multiplex luminex assay, and infection intensity was determined before and at 1 year after praziquantel ( PZQ ) treatment using the Kato‐Katz method. Cellular responses were grouped and the relationship between groups of correlated cellular responses and infection intensity before and after PZQ treatment was investigated. AWA and SEA induced mainly Th2 responses. In contrast, rSmLy6B, rSmTSP6 and rSmTSP7 induced Th1/pro‐inflammatory responses. While recombinant antigens rSmKK7 and rSmLy6A did not induce a Th1/pro‐inflammatory response, they had an association with pre‐treatment infection intensity after adjusting for age and sex. Testing more schistosomula antigens using this approach could provide immune‐epidemiology identifiers necessary for prioritizing next generation schistosomiasis vaccine candidates.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Schistosoma Mansoni Adult Worm and Egg Antigens Induce Th2mentioning

confidence: 99%

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Schistosoma mansoni schistosomula antigens induce Th1/Pro‐inflammatory cytokine responses

Egesa

Lubyayi

Tukahebwa

et al. 2018

Parasite Immunology

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“…SmVALs can be divided into two groups and exhibit various gene expression patterns throughout the entire life-cycle, including genes exclusively expressed in stages involved in intermediate host invasion or definitive host invasion and ubiquitously expressed genes [32]. Because of their potential functional classification, expression patterns and localization, SmVALs have been proposed as potential drug targets and vaccine candidates, and some members have been well characterized recently [66][67][68]. For instance, Farias et al have demonstrated that SmVAL4 and SmVAL26 stimulate differential allergic responses in a murine model of airway inflammation [66]; and in a recent study, egg-derived SmVAL9 was found to carry an N-linked glycan containing a schistosome-specific difucosyl element and function as an immunogenic target during chronic murine schistosomiasis [68].…”

Section: Phylogenetic Analysis and Global Expression Profiling Of Thementioning

confidence: 99%

“…Because of their potential functional classification, expression patterns and localization, SmVALs have been proposed as potential drug targets and vaccine candidates, and some members have been well characterized recently [66][67][68]. For instance, Farias et al have demonstrated that SmVAL4 and SmVAL26 stimulate differential allergic responses in a murine model of airway inflammation [66]; and in a recent study, egg-derived SmVAL9 was found to carry an N-linked glycan containing a schistosome-specific difucosyl element and function as an immunogenic target during chronic murine schistosomiasis [68]. In our study, 20 non-redundant S. japonicum venom-allergen-like protein (SjVAL) genes were identified in the genome.…”

Section: Phylogenetic Analysis and Global Expression Profiling Of Thementioning

confidence: 99%

Comparative Analysis of Transcriptional Profiles of Adult Schistosoma japonicum from Different Laboratory Animals and the Natural Host, Water Buffalo

et al. 2015

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BackgroundSchistosomiasis is one of the most widely distributed parasitic diseases in the world. Schistosoma japonicum, a zoonotic parasite with a wide range of mammalian hosts, is one of the major pathogens of this disease. Although numerous studies on schistosomiasis japonica have been performed using laboratory animal models, systematic comparative analysis of whole-genome expression profiles in parasites from different laboratory animals and nature mammalian hosts is lacking to date.Methodology/Principal FindingsAdult schistosomes were obtained from laboratory animals BALB/c mice, C57BL/6 mice, New Zealand white rabbits and the natural host, water buffaloes. The gene expression profiles of schistosomes from these animals were obtained and compared by genome-wide oligonucleotide microarray analysis. The results revealed that the gene expression profiles of schistosomes from different laboratory animals and buffaloes were highly consistent (r>0.98) genome-wide. Meanwhile, a total of 450 genes were identified to be differentially expressed in schistosomes which can be clustered into six groups. Pathway analysis revealed that these genes were mainly involved in multiple signal transduction pathways, amino acid, energy, nucleotide and lipid metabolism. We also identified a group of 1,540 abundantly and stably expressed gene products in adult worms, including a panel of 179 Schistosoma- or Platyhelminthes-specific genes that may be essential for parasitism and may be regarded as novel potential anti-parasite intervention targets for future research.Conclusions/SignificanceThis study provides a comprehensive database of gene expression profiles of schistosomes derived from different laboratory animals and water buffaloes. An expanded number of genes potentially affecting the development of schistosomes in different animals were identified. These findings lay the foundation for schistosomiasis research in different laboratory animals and natural hosts at the transcriptional level and provide a valuable resource for screening anti-schistosomal intervention targets.

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Extracellular Vesicle Biomarkers for Infectious Diseases

Zhang,

Yuan,

Giri

et al. 2024

Extracellular Vesicles

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Excreted/secreted Schistosoma mansoni venom allergen-like 9 (SmVAL9) modulates host extracellular matrix remodelling gene expression

Abstract: Graphical abstract

Cited by 40 publications

References 72 publications

Schistosoma mansoni schistosomula antigens induce Th1/Pro‐inflammatory cytokine responses

Schistosoma mansoni schistosomula antigens induce Th1/Pro‐inflammatory cytokine responses

Comparative Analysis of Transcriptional Profiles of Adult Schistosoma japonicum from Different Laboratory Animals and the Natural Host, Water Buffalo

Extracellular Vesicle Biomarkers for Infectious Diseases

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