Excitation-induced dynamics of external pH pattern in Chara corallina cells and its dependence on external calcium concentration

Eremin, Alexey; Булычев, А. А.; Krupenina, Natalia A.; Mair, Thomas; Hauser, M.; Stannarius, Ralf; Müller, Stefan; Rubin, A. B.

doi:10.1039/b607602e

Cited by 16 publications

(8 citation statements)

References 26 publications

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“…During an action potential, streaming is momentarily halted and the alkaline bands disappear. As streaming recovers, the alkaline bands tend to reform at the same position on the cell's surface and the recurrence was increased with the concentration of Ca 2þ in the surrounding medium [153].…”

Section: Alkaline Band Formation and Carbon Fixationmentioning

confidence: 99%

A physical perspective on cytoplasmic streaming

2015

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Organisms show a remarkable range of sizes, yet the dimensions of a single cell rarely exceed 100 µm. While the physical and biological origins of this constraint remain poorly understood, exceptions to this rule give valuable insights. A well-known counterexample is the aquatic plant Chara, whose cells can exceed 10 cm in length and 1 mm in diameter. Two spiralling bands of molecular motors at the cell periphery drive the cellular fluid up and down at speeds up to 100 µm s−1, motion that has been hypothesized to mitigate the slowness of metabolite transport on these scales and to aid in homeostasis. This is the most organized instance of a broad class of continuous motions known as ‘cytoplasmic streaming’, found in a wide range of eukaryotic organisms—algae, plants, amoebae, nematodes and flies—often in unusually large cells. In this overview of the physics of this phenomenon, we examine the interplay between streaming, transport and cell size and discuss the possible role of self-organization phenomena in establishing the observed patterns of streaming.

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Section: Alkaline Band Formation and Carbon Fixationmentioning

confidence: 99%

A physical perspective on cytoplasmic streaming

2015

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“…Laboratory studies indicate that precipitation to carbonate surfaces on the flow bed does not occur in the absence of biofilm (Shiraishi et al, 2008a, b;Pedley et al, 2009), and that photosynthesis may be critical to overcoming the energetic barrier to precipitation (Shiraishi et al, 2008a). This concept is given strong support by repeated observation of calcification of Charophyta bodies within water masses otherwise incapable of precipitating calcite (Anadon et al, 2002;Eremin et al, 2007;Pentecost et al, 2006). However, these effects still await proper quantification.…”

Section: Photosynthesis and Respirationmentioning

confidence: 99%

Linking mineralisation process and sedimentary product in terrestrial carbonates using a solution thermodynamic approach

et al. 2014

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Abstract. Determining the processes which generate terrestrial carbonate deposits (tufas, travertines and to a lesser extent associated chemical sediments such as calcretes and speleothems) is a long-standing problem. Precipitation of mineral products from solution reflects a complex combination of biological, equilibrium and kinetic processes, and the different morphologies of carbonate sediment produced by different processes have yet to be clearly demarked. Building on the groundbreaking work of previous authors, we propose that the underlying control on the processes leading to the deposition of these products can be most parsimoniously understood from the thermodynamic properties of their source solutions. Here, we report initial observations of the differences in product generated from spring and lake systems spanning a range of temperature–supersaturation space. We find that at high supersaturation, biological influences are masked by high rates of physico-chemical precipitation, and sedimentary products from these settings infrequently exhibit classic "biomediated" fabrics such as clotted micrite. Likewise, at high temperature (>40 °C) exclusion of vascular plants and complex/diverse biofilms can significantly inhibit the magnitude of biomediated precipitation, again impeding the likelihood of encountering the "bio-type" fabrics. Conversely, despite the clear division in product between extensive tufa facies associations and less spatially extensive deposits such as oncoid beds, no clear division can be identified between these systems in temperature–supersaturation space. We reiterate the conclusion of previous authors, which demonstrate that this division cannot be made on the basis of physico-chemical characteristics of the solution alone. We further provide a new case study of this division from two adjacent systems in the UK, where tufa-like deposition continuous on a metre scale is happening at a site with lower supersaturation than other sites exhibiting only discontinuous (oncoidal) deposition. However, a strong microbiological division is demonstrated between these sites on the basis of suspended bacterial cell distribution, which reach a prominent maximum where tufa-like deposits are forming. We conclude that at high supersaturation, the thermodynamic properties of solutions provide a highly satisfactory means of linking process and product, raising the opportunity of identifying water characteristics from sedimentological/petrological characteristics of ancient deposits. At low supersaturation, we recommend that future research focuses on geomicrobiological processes rather than the more traditional, inorganic solution chemistry approach dominant in the past.

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“…To maintain pH homeostasis of the cytoplasm, outward directed proton current is balanced by H + influx or OH − efflux (high pH channels; Bisson and Walker ). Previous studies suggest that the high pH channels are activated by metabolites released from photosynthetically active chloroplasts (Eremin et al , Bulychev and Komarova ).…”

Section: Introductionmentioning

confidence: 97%

Inhibition of endosomal trafficking by brefeldin A interferes with long‐distance interaction between chloroplasts and plasma membrane transporters

Булычев

Foissner

2019

Physiologia Plantarum

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The huge internodal cells of the characean green algae are a convenient model to study long-range interactions between organelles via cytoplasmic streaming. It has been shown previously that photometabolites and reactive oxygen species released by illuminated chloroplasts are transmitted to remote shaded regions where they interfere with photosynthetic electron transport and the differential activity of plasma membrane transporters, and recent findings indicated the involvement of organelle trafficking pathways. In the present study, we applied pulse amplitude-modulated microscopy and pH-sensitive electrodes to study the effect of brefeldin A (BFA), an inhibitor of vesicle trafficking, on long-distance interactions in Chara australis internodal cells. These data were compared with BFA-induced changes in organelle number, size and distribution using fluorescent dyes and confocal laser scanning microscopy. We found that BFA completely and immediately inhibited endocytosis in internodal cells and induced the aggregation of organelles into BFA compartments within 30-120 min of treatment. The comparison with the physiological data suggests that the early response, the arrest of endocytosis, is related to the attenuation of differences in surface pH, whereas the longer lasting formation of BFA compartments is probably responsible for the acceleration of the cyclosis-mediated interaction between chloroplasts. These data indicate that intracellular turnover of membrane material might be important for the circulation of electric currents between functionally distinct regions in illuminated characean internodes and that translational movement of metabolites is delayed by transient binding of the transported substances to organelles. 122

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Excitation-induced dynamics of external pH pattern in Chara corallina cells and its dependence on external calcium concentration

Cited by 16 publications

References 26 publications

A physical perspective on cytoplasmic streaming

A physical perspective on cytoplasmic streaming

Linking mineralisation process and sedimentary product in terrestrial carbonates using a solution thermodynamic approach

Inhibition of endosomal trafficking by brefeldin A interferes with long‐distance interaction between chloroplasts and plasma membrane transporters

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