Excessive Nitrite Affects Zebrafish Valvulogenesis through Yielding Too Much NO Signaling

Li, Junbo; Jia, Wenshuang; Zhao, Qingshun

doi:10.1371/journal.pone.0092728

Cited by 15 publications

(13 citation statements)

References 39 publications

(51 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To observe the morphology and count heart rates, we treated embryos with 0.04 mg/ml MS-222 (Sigma) at room temperature for 5 min and then mounted them with 3% methyl cellulose (Sigma). To examine the EI, we measured the distance from the heart center to the heart boundary (a) and from the heart center to the pericardium (b), as reported previously (28). The EI was calculated by the expression, b/a.…”

Section: Live Imaging and Quantificationmentioning

confidence: 99%

The transcription factor Foxc1a in zebrafish directly regulates expression of nkx2.5, encoding a transcriptional regulator of cardiac progenitor cells

Yue

Jiang

et al. 2018

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

Cardiogenesis is a tightly controlled biological process required for formation of a functional heart. The transcription factor Foxc1 not only plays a crucial role in outflow tract development in mice, but is also involved in cardiac structure formation and normal function in humans. However, the molecular mechanisms by which Foxc1 controls cardiac development remain poorly understood. Previously, we reported that zebrafish embryos deficient in , an ortholog of mammalian, display pericardial edemas and die 9-10 days postfertilization. To further investigate Foxc1a's role in zebrafish cardiogenesis and identify its downstream target genes during early heart development, we comprehensively analyzed the cardiovascular phenotype of -null zebrafish embryos. Our results confirmed that-null mutants exhibit disrupted cardiac morphology, structure, and function. Performing transcriptome analysis on the mutants, we found that the expression of the cardiac progenitor marker gene was significantly decreased, but the expression of germ layer-patterning genes was unaffected. Dual-fluorescence hybridization assays revealed that and are co-expressed in the anterior lateral plate mesoderm at the somite stage. Chromatin immunoprecipitation and promoter truncation assays disclosed that Foxc1a regulates expression via direct binding to two noncanonical binding sites in the proximal promoter. Moreover, functional rescue experiments revealed that developmental stage-specific overexpression partially rescues the cardiac defects of the -null embryos. Taken together, our results indicate that during zebrafish cardiogenesis, Foxc1a is active directly upstream of.

show abstract

Section: Live Imaging and Quantificationmentioning

confidence: 99%

The transcription factor Foxc1a in zebrafish directly regulates expression of nkx2.5, encoding a transcriptional regulator of cardiac progenitor cells

Yue

Jiang

et al. 2018

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…Work in zebrafish suggests that correct endocardium formation is exquisitely sensitive to NO. Embryos exposed to high nitrite concentrations form a normal vascular network and chamber endocardium but develop specific defects in the valve leaflets [73] .…”

Section: Role Of Mechanical Forces In Endocardium Development and Valmentioning

confidence: 99%

Interplay between cardiac function and heart development

Andrés-Delgado

Mercader

2016

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

100

View full text Add to dashboard Cite

Mechanotransduction refers to the conversion of mechanical forces into biochemical or electrical signals that initiate structural and functional remodeling in cells and tissues. The heart is a kinetic organ whose form changes considerably during development and disease. This requires cardiomyocytes to be mechanically durable and able to mount coordinated responses to a variety of environmental signals on different time scales, including cardiac pressure loading and electrical and hemodynamic forces. During physiological growth, myocytes, endocardial and epicardial cells have to adaptively remodel to these mechanical forces. Here we review some of the recent advances in the understanding of how mechanical forces influence cardiac development, with a focus on fluid flow forces. This article is part of a Special Issue entitled: Cardiomyocyte Biology: Integration of Developmental and Environmental Cues in the Heart edited by Marcus Schaub and Hughes Abriel.

show abstract

“…To understand the toxic effects of nitrite on human heart, Li exposed zebrafish embryos to different concentrations of sodium nitrite, their results showed that sodium nitrite caused the developmental defects of zebrafish heart dose-dependently. [18] In recent years, Penicillium spp. pigments have attracted the attention of researchers.…”

Section: Introductionmentioning

confidence: 99%

Chemical Composition, Security and Bioactivity of the Red Pigment from Penicillium purpurogenum Li‐3

Jin

Zhang

Cao

et al. 2018

Chemistry & Biodiversity

View full text Add to dashboard Cite

The chemical composition, security and bioactivity of pigments from Penicillium purpurogenum Li-3 strain screened by our group were firstly studied in this work. DPPH and the filter disc diffusion method were used to determine the biological activities of the red pigments. The pigment was characterized by UV/VIS, FT-IR, NMR and UPLC-Q-TOF-MS. HPLC/MS was used to detect mycotoxins (citrinin) in fermentation broth. An acute toxicity was detected in the embryos of zebrafish. As a consequence, the crude red pigment from the AcOEt fraction showed better DPPH scavenge capacity and antibacterial activity. Spectroscopic (UV, FT-IR, C-NMR) and UPLC-Q-TOF-MS analysis revealed that the Penicillium purpurogenum Li-3 red pigment (RPs) was monascus-like pigment and its molecular weight was 439.1997. Moreover, the red pigment was shown to be weak cytotoxic against the zebrafish embryos. The yield of the red pigment increased 69 % under optimized culture conditions. These outstanding properties will enlarge the application of RPs for natural food additives, new antioxidant and antibacterial drug development.

show abstract

Excessive Nitrite Affects Zebrafish Valvulogenesis through Yielding Too Much NO Signaling

Cited by 15 publications

References 39 publications

The transcription factor Foxc1a in zebrafish directly regulates expression of nkx2.5, encoding a transcriptional regulator of cardiac progenitor cells

The transcription factor Foxc1a in zebrafish directly regulates expression of nkx2.5, encoding a transcriptional regulator of cardiac progenitor cells

Interplay between cardiac function and heart development

Chemical Composition, Security and Bioactivity of the Red Pigment from Penicillium purpurogenum Li‐3

Contact Info

Product

Resources

About