Examination of Effective Culture Methods for Rabbit Preantral Follicles

Sugimoto, Hironobu; Miyamoto, Yuki; Tsuji, Yoko; Morimoto, Kouichi; Taniguchi, Takeshi; Morimoto, Yoshiharu; Hosoi, Yoshihiko

doi:10.1274/jmor.26.221

Cited by 2 publications

(3 citation statements)

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“…In general, IVG–IVM oocytes show poor development after fertilization compared with ovulated oocytes; therefore, IVG in mammals has been studied extensively to achieve complete growth and development (Martins et al ., 2008; Silva et al ., 2010; Sugimoto et al ., 2009, 2012). In the present study, the blastocyst rate after SCNT using IVG–IVM oocytes improved by approximately two-fold by TSA treatment; this effect is similar to that of TSA when SCNT is performed using ovulated oocytes.…”

Section: Discussionmentioning

confidence: 99%

“…If these oocytes could be grown to reach meiotic competence in vitro , they would provide a large potential source for protection of endangered species. In vitro growth (IVG) of oocytes has been researched and reported in mice (Nayudu & Osborn, 1992; Johnson et al ., 1995; Cortvrindt et al ., 1996; Eppig & O'Brien, 1996), pig (Hirao et al ., 1994; Hashimoto et al ., 2007b), cattle (Gutierrez et al ., 2000), and rabbits (Kanaya et al ., 2007; Sugimoto et al ., 2009, 2012). In mice (Eppig & Schroeder, 1989) and cattle (Yamamoto et al ., 1999; Hirao et al ., 2004), there have been reports of successful production of offspring from mature oocytes after IVG, in vitro maturation (IVM), and in vitro fertilization.…”

Section: Introductionmentioning

confidence: 99%

“…4 Kitayama Labes Co. Ltd., Ina Research Laboratory, Ina, Nagano 396-0021, Japan. et al, 1995;Cortvrindt et al, 1996;Eppig & O'Brien, 1996), pig (Hirao et al, 1994;Hashimoto et al, 2007b), cattle (Gutierrez et al, 2000), and rabbits (Kanaya et al, 2007;Sugimoto et al, 2009Sugimoto et al, , 2012. In mice (Eppig & Schroeder, 1989) and cattle (Yamamoto et al, 1999;Hirao et al, 2004), there have been reports of successful production of offspring from mature oocytes after IVG, in vitro maturation (IVM), and in vitro fertilization.…”

Section: Introductionmentioning

confidence: 99%

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Production of somatic cell nuclear transfer embryos using in vitro-grown and in vitro-matured oocytes in rabbits

Sugimoto

Kida

et al. 2014

Zygote

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We examined growing oocytes collected from follicles remaining in superovulated rabbit ovaries, that were grown (in vitro growth, IVG) and matured (in vitro maturation, IVM) in vitro. We produced somatic cell nuclear transfer (SCNT) embryos using the mature oocytes and examined whether these embryos have the ability to develop to the blastocyst stage. In addition, we examined the effects of trichostatin A (TSA), a histone deacetylase inhibitor (HDACi), on the developmental competence of SCNT embryos derived from IVG-IVM oocytes. After growth for 7 days and maturation for 14-16 h in vitro, the growing oocytes reached the metaphase II stage (51.4%). After SCNT, these reconstructed embryos reached the blastocyst stage (20%). Furthermore, the rate of development to the blastocyst stage and the number of cells in the blastocysts in SCNT embryos derived from IVG-IVM oocytes were significantly higher for TSA-treated embryos compared with TSA-untreated embryos (40.6 versus 21.4% and 353.1 ± 59.1 versus 202.5 ± 54.6, P < 0.05). These results indicate that rabbit SCNT embryos using IVG-IVM oocytes have the developmental competence to reach the blastocyst stage.

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