Ex-vivo Effect of Intracanal Medications Based on Ozone and Calcium Hydroxide in Root Canals Contaminated with Enterococcus faecalis

Farac, Roberta Vieira; Pizzolitto, Antônio Carlos; Guerreiro-Tanomaru, Juliane Maria; Morgental, Renata Dornelles; Lima, Regina Karla de Pontes; Bonetti-Filho, Idomeo

doi:10.1590/0103-6440201301992

Cited by 12 publications

(9 citation statements)

References 22 publications

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“…Additionally, ozone has other advantages like such as improving wound healing, enhancing immune, no side effect, no-toxic, environmental friendly and high efficacy ( 12 ). A few studies have shown that ozone therapy is efficient in killing many kinds of microorganisms, such as S. aureus , Streptococci spp, Escherichia coli , Enterococcus faecalis , and P. aeruginosa ( 13 , 14 ).…”

Section: Introductionmentioning

confidence: 99%

The antibacterial effect of topical ozone on the treatment of MRSA skin infection

et al. 2017

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Skin can be infected by many types of microorganisms, most commonly by gram-positive strains of Staphylococcus and Streptococcus spp. Treatment of Staphylococcus aureus (S. aureus) infections, particularly that of methicillin resistant Staphylococcus aureus (MRSA), is a challenge in clinical practice. Ozone therapy has proven to be one of the strongest antiseptics against the majority of microorganisms involved in skin infections. The purpose of the present study was to evaluate the microbicidal effects of topical ozone therapy on S. aureus and MRSA, and determine the clinical efficacy of ozone therapy on patients with MRSA skin infection. Microbicidal effects of ozonated oil and ozonated water were determined by plating and Kirby Bauer methods. Clinical efficacy and safety of topical ozone were evaluated in two cases with skin MRSA infection. The killing rates of ozonated oil for S. aureus and MRSA were greater when compared with the control oil group. Almost 100% of S. aureus were eliminated by ozonated oil following 5 min. Almost 100% MRSA were eliminated by ozonated oil following 15 min. In addition, 100% S. aureus and 100% MRSA were eliminated by ozonated water in 1 min. The inhibition zone diameters of ozonated oil for S. aureus and MRSA were 17 and 13 mm, respectively, which were significantly larger than the control group. Both cases of skin MRSA infection were completely healed with ozone therapy. In conclusion, ozone therapy is a potential treatment for S. aureus and MRSA skin infection as it has great efficacy, few side effects and low-costs.

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Section: Introductionmentioning

confidence: 99%

The antibacterial effect of topical ozone on the treatment of MRSA skin infection

et al. 2017

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“…Another factor that may corroborate the results obtained in this study is the ephemeral ozone gas half-life. 24 The only techniques that have been tested so far and have showed a certain degree of effectiveness against LPS are (a) the use of calcium hydroxide as an intra-canal medication 12,25,26 as it hydrolyses lipid A and changes it into chains of fatty acid and nontoxic sugars, 27 and (b) the application of laser Nd:YAG. 9 The results obtained in this study reinforce the importance of instrumentation in addition to any disinfection protocol, 11,28,29 especially with regard to bacterial LPS that strongly adheres to the dentinal wall 6 and makes it necessary to use endodontic instruments for its removal.…”

Section: Discussionmentioning

confidence: 99%

LPS levels in root canals after the use of ozone gas and high frequency electrical pulses

et al. 2016

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The present study aims to verify the effect of ozone gas (OZY ® System) and high frequency electric pulse (Endox ® System) systems on human root canals previously contaminated with Escherichia coli lipopolysaccharide (LPS). Fifty single-rooted teeth had their dental crowns removed and root lengths standardized to 16 mm. The root canals were prepared up to #60 hand K-files and sterilized using gamma radiation with cobalt 60. The specimens were divided into the following five groups (n = 10) based on the disinfection protocol used: OZY ® System, one 120-second-pulse (OZY 1p); OZY ® System, four 24-second-pulses (OZY 4p); and Endox ® System (ENDOX). Contaminated and non-contaminated canals were exposed only to apyrogenic water and used as positive (C+) and negative (C-) controls, respectively. LPS (O55:B55) was administered in all root canals except those belonging to group C-. After performing disinfection, LPS samples were collected from the canals using apyrogenic paper tips. Limulus Amoebocyte Lysate (LAL) was used to quantify the LPS levels, and the data obtained was analyzed using one-way ANOVA. The disinfection protocols used were unable to reduce the LPS levels significantly (p = 0.019). The use of ozone gas and high frequency electric pulses was not effective in eliminating LPS from the root canals.

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“…Nekoliko metoda, kao što su korištenje raznih tehnika instrumentacije kanala (3), tehnike irigacije antimikrobnim otopinama (4) i intrakanalnih medikamenata (5), opisane su kako bi se postigla učinkovita intrakanalna dezinfekcija.…”

Section: Uvodunclassified

Evaluation of Microbial Reduction in Root Canals Instrumented with Reciprocating and Rotary Systems

Oliveira

Aguiar

Câmara

et al. 2015

Acta Stomatol Croat

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Ex-vivo Effect of Intracanal Medications Based on Ozone and Calcium Hydroxide in Root Canals Contaminated with Enterococcus faecalis

Cited by 12 publications

References 22 publications

The antibacterial effect of topical ozone on the treatment of MRSA skin infection

The antibacterial effect of topical ozone on the treatment of MRSA skin infection

LPS levels in root canals after the use of ozone gas and high frequency electrical pulses

Evaluation of Microbial Reduction in Root Canals Instrumented with Reciprocating and Rotary Systems

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