Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant species

Trujillo, Aura Inés Urrea; Giraldo, Catalina Botero; Gòmez, Esther Julia Naranjo

doi:10.15446/acag.v66n4.60080

Cited by 3 publications

(1 citation statement)

References 21 publications

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“…Plants that are transferred from in vitro to greenhouse conditions need to acclimatise to drier and less nutrient-rich soil by protecting their water content via the stomatal closure and by activating photosynthesis [47,56,57]. These adaptations make the transfer the most critical step for the survival of in vitro cultivated plants and a high percentage of plants may get lost [57–61]. Most of the Burkholderia- free plantlets did not survive under greenhouse conditions and often died after the degeneration of the shoot apical meristem, or due to low resistance against aphids and fungal infections.…”

Section: Discussionmentioning

confidence: 99%

Is the bacterial leaf nodule symbiosis obligate for Psychotria umbellata? The development of a Burkholderia-free host plant

et al. 2019

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Background & aims The bacterial leaf nodule symbiosis is an interaction where bacteria are housed in specialised structures in the leaves of their plant host. In the Rubiaceae plant family, host plants interact with Burkholderia bacteria. This interaction might play a role in the host plant defence system. It is unique due to its high specificity; the vertical transmission of the endophyte to the next generation of the host plant; and its supposedly obligatory character. Although previous attempts have been made to investigate this obligatory character by developing Burkholderia -free plants, none have succeeded and nodulating plants were still produced. In order to investigate the obligatory character of this endosymbiosis, our aims were to develop Burkholderia- free Psychotria umbellata plants and to investigate the effect of the absence of the endophytes on the host in a controlled environment. Methods The Burkholderia- free plants were obtained via embryo culture, a plant cultivation technique. In order to analyse the endophyte-free status, we screened the plants morphologically, microscopically and molecularly over a period of three years. To characterise the phenotype and growth of the in vitro aposymbiotic plants, we compared the growth of the Burkholderia -free plants to the nodulating plants under the same in vitro conditions. Key results All the developed plants were Burkholderia -free and survived in a sterile in vitro environment. The growth analysis showed that plants without endophytes had a slower development. Conclusions Embryo culture is a cultivation technique with a high success rate for the development of Burkholderia -free plants of P . umbellata . The increased growth rate in vitro when the specific endophyte is present cannot be explained by possible benefits put forward in previous studies. This might indicate that the benefits of the endosymbiosis are not yet completely understood.

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Section: Discussionmentioning

confidence: 99%

Is the bacterial leaf nodule symbiosis obligate for Psychotria umbellata? The development of a Burkholderia-free host plant

et al. 2019

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Cinchona officinalis L. ex situ conservation by in vitro slow growth and cryopreservation techniques

Armijos-González,

Ramón,

Cueva-Agila

2024

Plant Cell Tiss Organ Cult

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Cinchona officinalis L. in vitro conservation by means of slow-growth storage and cryopreservation techniques

Armijos-Gonzalez,

Ramón-Contento,

Cueva-Agila

2023

Preprint

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Cinchona officinalis has experienced anthropogenic pressures for nearly 400 years, such as overexploitation, habitat fragmentation, and the subsequent reduction of genetic diversity. Additionally, the challenge of regeneration in its natural environment makes it a vulnerable species. In this context, various treatments for the in vitro conservation of explants were evaluated in the present study. For short-term conservation, the effects of osmotic substances such as sorbitol, mannitol, and sucrose at different concentrations were assessed. Different concentrations of MS and B5 culture media were also examined for their impact on the growth, budding, mortality, and rooting of explants over 12 months without subcultures. For long-term conservation by cryopreservation, two techniques were tested: vitrification and encapsulation-dehydration. Short-term preservation of explants in sorbitol resulted in low mortality, minimal growth, and limited development of new shoots compared to preservation in sucrose or mannitol, although tissues could be recovered successfully from all storage conditions. After cryopreservation and 45 days of recovery, explants with the lowest mortality (4%) were from the control treatment, cultivated in a medium with sucrose which proved useful as a cryoprotectant, followed by the encapsulation-dehydration treatment. In conclusion, it is possible to conserve C. officinalis tissues in the short-term using in vitro techniques, while further assays are needed for long-term conservation.

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Ex situ potential conservation of ipecacuanha (Psychotria ipecacuanha (Brot.) Stokes.), a critically endangered medicinal plant species

Cited by 3 publications

References 21 publications

Is the bacterial leaf nodule symbiosis obligate for Psychotria umbellata? The development of a Burkholderia-free host plant

Is the bacterial leaf nodule symbiosis obligate for Psychotria umbellata? The development of a Burkholderia-free host plant

Cinchona officinalis L. ex situ conservation by in vitro slow growth and cryopreservation techniques

Cinchona officinalis L. in vitro conservation by means of slow-growth storage and cryopreservation techniques

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