Evolutionary conservation of KLF transcription factors and functional conservation of human γ-globin gene regulation in chicken☆

Basu, P.; Sargent, Thanh Giang; Redmond, Latasha C.; Aisenberg, Jeremy C.; Kransdorf, E.; Wang, Shou Zhen; Ginder, Gordon D.; Lloyd, Joyce A.

doi:10.1016/j.ygeno.2004.02.013

Cited by 22 publications

(21 citation statements)

References 40 publications

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“…However, it was expected that the chicken have an EKLF gene, because the mammalian globin genes are correctly regulated in chicken cells, and vice versa. In fact, a complex binding specifically to globin CACCC boxes was observed with chicken adult blood nuclear extracts in gel-shift assays (Basu et al, 2004). In the current study, we have identified the chicken homologue of the mammalian EKLF gene and examined its tissue specificity and expression pattern during development.…”

Section: Discussionmentioning

confidence: 99%

“…Previous work in our laboratory identified many KLFs in the chicken with homology to the human KLFs (Basu et al, 2004), but interestingly, no homologue of EKLF was found. In this study, an erythroid-specific gene in the chicken with homology to EKLF has been identified.…”

Section: Introductionmentioning

confidence: 86%

“…There are 17 KLFs that have been identified in mammals, and they are related to the SP1-like family of transcription factors, with one overlap in nomenclature (SP6/KLF14; Philipsen and Suske, 1999;Turner and Crossley, 1999;Bieker, 2001;Black et al, 2001;Kaczynski et al, 2003). We previously have identified chicken homologues of the human KLF2, 3,4,5,9,11,12,13, and 15 genes, and evolutionary conservation of these genes suggests functional conservation (Basu et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…The regulation of human, murine, and avian ␤-like globin genes suggests that they are controlled by similar factors (Reitman et al, 1990;Glauber et al, 1991;Foley et al, 1994;Mason et al, 1995;Basu et al, 2004). In experiments both in a mouse erythroleukemia (MEL) cell line and in transgenic mice, the globin genes in a construct with the entire chicken ␤-globin locus were developmentally regulated and exhibited a pattern of expression mirroring that seen in the chicken (Glauber et al, 1991;Mason et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

“…Another study demonstrated that a construct containing the chicken ⑀-globin gene and the ␤/⑀ enhancer exhibited autonomous, highlevel stage-and tissue-specific expression in transgenic mice (Foley et al, 1994). When the human ␥-and ␤-globin genes are transiently transfected into primitive chicken blood cells, the ␥-globin gene is expressed and ␤-globin transcription is suppressed; that is, the genes are correctly regulated (Basu et al, 2004). These findings suggest that common factor(s) direct developmental expression of the globin genes in the chicken, in mouse, and in human cells.…”

Section: Introductionmentioning

confidence: 99%

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Identification, characterization, and expression pattern of the chicken EKLF gene

Chervenak¹,

Basu²,

Shin³

et al. 2006

Developmental Dynamics

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EKLF/KLF1 was the first of the Krü ppel-like factors (KLFs) to be identified in mammals and plays an important role in primitive and definitive erythropoiesis. Here, we identify and characterize EKLF in the chicken (cEKLF). The predicted amino acid sequence of the zinc finger region of cEKLF is at least 87.7% similar to mammalian EKLF proteins and is 98.8% and 95% similar to the EKLF orthologues in Xenopus and zebrafish, respectively. During early embryonic development, cEKLF expression is seen in the posterior primitive streak, which gives rise to hematopoietic cells, and then in the blood islands and in circulating blood cells. cEKLF mRNA is expressed in blood cells but not in brain later in chicken embryonic development. cEKLF mRNA is increased in definitive compared with primitive erythropoiesis. The conserved sequence and expression pattern of cEKLF suggests that its function is similar to its orthologues in mammals, Xenopus, and zebrafish.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Identification, characterization, and expression pattern of the chicken EKLF gene

Chervenak¹,

Basu²,

Shin³

et al. 2006

Developmental Dynamics

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Embryonic expression of the chicken krüppel‐like (KLF) transcription factor gene family

Antin

Pier

Sesepasara

et al. 2010

Developmental Dynamics

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The Krü ppel-like transcription factors (KLF) are zinc finger proteins that activate and suppress target gene transcription. Although KLF factors have been implicated in regulating many developmental processes, a comprehensive gene expression analysis has not been reported. Here we present the chicken KLF gene family and expression during the first five days of embryonic development. Fourteen chicken KLF genes or expressed sequences have been previously identified. Through synteny analysis and cDNA mapping, we have identified the KLF9 gene and determined that the gene presently named KLF1 is the true ortholog of KLF17 in other species. In situ hybridization expression analyses show that in general KLFs are broadly expressed in multiple cell and tissue types. Expression of KLFs 3, 7, 8, and 9, is widespread at all stages examined. KLFs 2, 4, 5, 6, 10, 11, 15, and 17 show more restricted patterns that suggest multiple functions during early stages of embryonic development.

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Gene Interactions Between Krüppel-like Factors in Development

Lloyd

2009

The Biology of Krüppel-Like Factors

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Evolutionary conservation of KLF transcription factors and functional conservation of human γ-globin gene regulation in chicken☆

Cited by 22 publications

References 40 publications

Identification, characterization, and expression pattern of the chicken EKLF gene

Identification, characterization, and expression pattern of the chicken EKLF gene

Embryonic expression of the chicken krüppel‐like (KLF) transcription factor gene family

Gene Interactions Between Krüppel-like Factors in Development

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