Evolution of oxygen utilization in multicellular organisms and implications for cell signalling in tissue engineering

Stamati, Katerina; Mudera, Vivek; Cheema, Umber

doi:10.1177/2041731411432365

Cited by 88 publications

(70 citation statements)

References 64 publications

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“…The stem cell niche provides a microenvironment conducive for stem cell survival and maintenance of multipotency. Within the bone marrow, the physiological site of the MSCs niche environment, reported levels of physiological pO 2 range between 0 and 7% [20,39]. MSCs within rolled collagen scaffolds generated physiological hypoxia due to the formation of O 2 consumption gradients [25].…”

Section: Discussionmentioning

confidence: 99%

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Delivery of mesenchymal stem cells in biomimetic engineered scaffolds promotes healing of diabetic ulcers

Assi

Foster

et al. 2016

Regenerative Medicine

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Aim:We hypothesized that delivery of mesenchymal stem cells (MSCs) in a biomimetic collagen scaffold improves wound healing in a diabetic mouse model. Materials & methods:Rolled collagen scaffolds containing MSCs were implanted or applied topically to diabetic C57BL/6 mice with excisional wounds. Results: Rolled scaffolds were hypoxic, inducing MSC synthesis and secretion of VEGF. Diabetic mice with wounds treated with rolled scaffolds containing MSCs showed increased healing compared with controls. Histologic examination showed increased cellular proliferation, increased VEGF expression and capillary density, and increased numbers of macrophages, fibroblasts and smooth muscle cells. Addition of laminin to the collagen scaffold enhanced these effects. Conclusion: Activated MSCs delivered in a biomimetic-collagen scaffold enhanced wound healing in a translationally relevant diabetic mouse model.

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Section: Discussionmentioning

confidence: 99%

“…Delivery of MSCs in biomimetic engineered scaffolds promotes healing of diabetic ulcers Research Article genic potential [19], which is not surprising since MSCs reside in the bone marrow niche in vivo that has lower oxygen tension compared with typical atmospheric conditions that are used for tissue culture [20].…”

mentioning

confidence: 99%

Delivery of mesenchymal stem cells in biomimetic engineered scaffolds promotes healing of diabetic ulcers

Assi

Foster

et al. 2016

Regenerative Medicine

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“…Glycolysis as means to generate energy is central to almost all known living organisms. However, it was the requisition of mitochondria by cells, and with this, the acquisition of OXPHOS in the presence of an atmosphere increasingly rich in oxygen that was the crucial step in metabolic evolution as this process provided the platform for sufficient energy generation to sustain complex multicellular life (96, 97). Interestingly, the TLRs and the NOD-like receptors appeared when the first primitive multicell layer animals, marine sponges (Demospongiae: Porifera), evolved.…”

Section: Early Coevolution Of Complement and Metabolismmentioning

confidence: 99%

Keeping It All Going—Complement Meets Metabolism

Kolev

Kemper²

2017

Front. Immunol.

371

544

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The complement system is an evolutionary old and crucial component of innate immunity, which is key to the detection and removal of invading pathogens. It was initially discovered as a liver-derived sentinel system circulating in serum, the lymph, and interstitial fluids that mediate the opsonization and lytic killing of bacteria, fungi, and viruses and the initiation of the general inflammatory responses. Although work performed specifically in the last five decades identified complement also as a critical instructor of adaptive immunity—indicating that complement’s function is likely broader than initially anticipated—the dominant opinion among researchers and clinicians was that the key complement functions were in principle defined. However, there is now a growing realization that complement activity goes well beyond “classic” immune functions and that this system is also required for normal (neuronal) development and activity and general cell and tissue integrity and homeostasis. Furthermore, the recent discovery that complement activation is not confined to the extracellular space but occurs within cells led to the surprising understanding that complement is involved in the regulation of basic processes of the cell, particularly those of metabolic nature—mostly via novel crosstalks between complement and intracellular sensor, and effector, pathways that had been overlooked because of their spatial separation. These paradigm shifts in the field led to a renaissance in complement research and provide new platforms to now better understand the molecular pathways underlying the wide-reaching effects of complement functions in immunity and beyond. In this review, we will cover the current knowledge about complement’s emerging relationship with the cellular metabolism machinery with a focus on the functional differences between serum-circulating versus intracellularly active complement during normal cell survival and induction of effector functions. We will also discuss how taking a closer look into the evolution of key complement components not only made the functional connection between complement and metabolism rather “predictable” but how it may also give clues for the discovery of additional roles for complement in basic cellular processes.

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“…1 Oxygen is the primary intracellular acceptor of electrons that are generated during mitochondrial respiration. Therefore, proper tissue function strictly relies on the constant delivery of adequate oxygen levels to all cellular environments in the organism.…”

Section: Oxygen In Tissues and Stem Cell Biologymentioning

confidence: 99%

The science behind the hypoxic niche of hematopoietic stem and progenitors

Nombela‐Arrieta

Silberstein

2014

Hematology

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In blood, oxygen is transported principally by hemoglobin tetrameric molecules in erythocytes, which allow for the delivery to tissue cells. When anemia occurs, such as perisurgically or after trauma, blood transfusion is administered to replace the deficit in oxygen-carrying capacity. During embryogenesis and later in adult life, tissue oxygen levels control multiple key cellular functions. Low tissue oxygen levels in particular are physiologically relevant to stem cells by controlling their metabolism and cell fate. In adult life, hematopoietic stem cells reside in specified BM microenvironments/niches, where their quiescence and differentiation are presumably also influenced by cell-intrinsic and cell-extrinsic (niche) factors. Novel imaging technologies have allowed determination of the spatial localization of hematopoietic stem/progenitor cells (HSPCs), as well as the topography of oxygen distribution in BM cavities. Together, these recent advances have contributed to the emergence of a novel model that challenges the previous concept of a hypoxic hematopoietic stem cell niche characterized by poorly perfused endosteal zones with the deepest hypoxia. HSPCs display a hypoxic phenotype despite residing in close association with arterial or sinusoidal vascular networks. The entire BM cavity is hypoxic and unexpectedly exhibits an opposite oxygen gradient to the one initially proposed because arteriole-rich endosteal zones are relatively less hypoxic than deeper regions of the BM perfused by dense sinusoidal networks. Therefore, further studies are warranted to elucidate to what extent differences in oxygen tensions in these diverse microenvironments influence HSPC homeostasis. Learning Objective• To understand the fundamental basic research carried out to investigate the relevance of hypoxic conditions in bone marrow microenvironments that support hematopoietic stem cell maintenance Oxygen in tissues and stem cell biologyDuring evolution, fluctuations in atmospheric oxygen concentration have defined eukaryotic life forms on earth, exemplified by the adoption of efficient oxidative phosphorylation (ie, aerobic metabolism) to support more complex and multicellular organisms. 1

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Evolution of oxygen utilization in multicellular organisms and implications for cell signalling in tissue engineering

Cited by 88 publications

References 64 publications

Delivery of mesenchymal stem cells in biomimetic engineered scaffolds promotes healing of diabetic ulcers

Delivery of mesenchymal stem cells in biomimetic engineered scaffolds promotes healing of diabetic ulcers

Keeping It All Going—Complement Meets Metabolism

The science behind the hypoxic niche of hematopoietic stem and progenitors

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