Evolution of Nanoparticle Protein Corona across the Blood–Brain Barrier

Cox, Alysia; Andreozzi, Patrizia; Magro, Roberta Dal; Fiordaliso, Fabio; Corbelli, Alessandro; Talamini, Laura; Chinello, Clizia; Raimondo, Francesca; Magni, Fulvio; Tringali, Maria; Krol, Silke; Silva, Paulo Jacob; Stellacci, Francesco; Masserini, Massimo; Re, Francesca

doi:10.1021/acsnano.8b03500

Cited by 150 publications

(100 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another important aspect is the characterization of nanoparticles under the envisioned operating conditions of the nanomaterial. One important lesson learned in the last decade is that in biological fluids proteins assemble with the nanoparticles, leading to a protein corona that gives a “biological identity” to the particles . As the application of nanoparticles in the biological context is a key technology, comprehensive knowledge of the physicochemical parameters under physiological conditions is of fundamental importance.…”

Section: Discussionmentioning

confidence: 99%

Nanoparticle Characterization: What to Measure?

et al. 2019

View full text Add to dashboard Cite

What to measure? is a key question in nanoscience, and it is not straightforward to address as different physicochemical properties define a nanoparticle sample. Most prominent among these properties are size, shape, surface charge, and porosity. Today researchers have an unprecedented variety of measurement techniques at their disposal to assign precise numerical values to those parameters. However, methods based on different physical principles probe different aspects, not only of the particles themselves, but also of their preparation history and their environment at the time of measurement. Understanding these connections can be of great value for interpreting characterization results and ultimately controlling the nanoparticle structure–function relationship. Here, the current techniques that enable the precise measurement of these fundamental nanoparticle properties are presented and their practical advantages and disadvantages are discussed. Some recommendations of how the physicochemical parameters of nanoparticles should be investigated and how to fully characterize these properties in different environments according to the intended nanoparticle use are proposed. The intention is to improve comparability of nanoparticle properties and performance to ensure the successful transfer of scientific knowledge to industrial real‐world applications.

show abstract

Section: Discussionmentioning

confidence: 99%

Nanoparticle Characterization: What to Measure?

et al. 2019

View full text Add to dashboard Cite

show abstract

“…• The presence of cell culture medium can influence PC composition. Bonvin et al, 2017Cox et al, 2018Ho et al, 2018…”

Section: Feature Influence On Np Interactions With Proteins Referencesmentioning

confidence: 99%

Recent Advances in Understanding the Protein Corona of Nanoparticles and in the Formulation of “Stealthy” Nanomaterials

Rampado

Crotti

Caliceti

et al. 2020

Front. Bioeng. Biotechnol.

245

166

View full text Add to dashboard Cite

In the last decades, the staggering progress in nanotechnology brought around a wide and heterogeneous range of nanoparticle-based platforms for the diagnosis and treatment of many diseases. Most of these systems are designed to be administered intravenously. This administration route allows the nanoparticles (NPs) to widely distribute in the body and reach deep organs without invasive techniques. When these nanovectors encounter the biological environment of systemic circulation, a dynamic interplay occurs between the circulating proteins and the NPs, themselves. The set of proteins that bind to the NP surface is referred to as the protein corona (PC). PC has a critical role in making the particles easily recognized by the innate immune system, causing their quick clearance by phagocytic cells located in organs such as the lungs, liver, and spleen. For the same reason, PC defines the immunogenicity of NPs by priming the immune response to them and, ultimately, their immunological toxicity. Furthermore, the protein corona can cause the physical destabilization and agglomeration of particles. These problems induced to consider the PC only as a biological barrier to overcome in order to achieve efficient NP-based targeting. This review will discuss the latest advances in the characterization of PC, development of stealthy NP formulations, as well as the manipulation and employment of PC as an alternative resource for prolonging NP half-life, as well as its use in diagnostic applications.

show abstract

“…On the other hand, various biobarriers may limit the extravasation of nanodrugs into solid tumor, and in certain cases, such as in the case of liposomal nanocarriers, the gain in the therapeutic index is primarily related to reduction of toxicity rather than to efficacy increase . Indeed, nanodrugs, including liposomes, must overcome various and sequential biobarriers to reach their targets, such as the endothelial barrier, the membrane of tumor cells, and subcellular organelles (e.g., nucleus and lysosomes); they must resist to enzymatic degradation in the blood, should avoid sequestration by phagocytes of the reticuloendothelial system (RES) and molecular efflux pumps, and must migrate against adverse oncotic and interstitial pressures in the tumor . Some of these barriers are related (or consequent) to the abnormal structure of the tumor vasculature, which is typically characterized by highly disorganized architecture, by vessels with dilated and excessive branching, shunts, and fenestrations, and by vessels lacking basement membrane or with fewer pericytes, compared to normal vessels .…”

Section: Introductionmentioning

confidence: 99%

Overcoming Biological Barriers in Neuroblastoma Therapy: The Vascular Targeting Approach with Liposomal Drug Nanocarriers

Pastorino

Brignole

Paolo

et al. 2019

Small

View full text Add to dashboard Cite

Neuroblastoma is a rare pediatric cancer characterized by a wide clinical behavior and adverse outcome despite aggressive therapies. New approaches based on targeted drug delivery may improve efficacy and decrease toxicity of cancer therapy. Furthermore, nanotechnology offers additional potential developments for cancer imaging, diagnosis, and treatment. Following these lines, in the past years, innovative therapies based on the use of liposomes loaded with anticancer agents and functionalized with peptides capable of recognizing neuroblastoma cells and/or tumor‐associated endothelial cells have been developed. Studies performed in experimental orthotopic models of human neuroblastoma have shown that targeted nanocarriers can be exploited for not only decreasing the systemic toxicity of the encapsulated anticancer drugs, but also increasing their tumor homing properties, enhancing tumor vascular permeability and perfusion (and, consequently, drug penetration), inducing tumor apoptosis, inhibiting angiogenesis, and reducing tumor glucose consumption. Furthermore, peptide‐tagged liposomal formulations are proved to be more efficacious in inhibiting tumor growth and metastatic spreading of neuroblastoma than nontargeted liposomes. These findings, herein reviewed, pave the way for the design of novel targeted liposomal nanocarriers useful for multitargeting treatment of neuroblastoma.

show abstract

Evolution of Nanoparticle Protein Corona across the Blood–Brain Barrier

Cited by 150 publications

References 48 publications

Nanoparticle Characterization: What to Measure?

Nanoparticle Characterization: What to Measure?

Recent Advances in Understanding the Protein Corona of Nanoparticles and in the Formulation of “Stealthy” Nanomaterials

Overcoming Biological Barriers in Neuroblastoma Therapy: The Vascular Targeting Approach with Liposomal Drug Nanocarriers

Contact Info

Product

Resources

About