Évolution des souches de Rotavirus du groupe A en circulation en Tunisie sur une période de trois ans (2005–2007)

Chouikha, Anissa; Fredj, M. Ben; Fodha, I.; Mathlouthi, I.; Ardhaoui, M.; Teleb, Nadia; Brini, Inès; Messaadi, F.; Mastouri, Maha; Sfar, T.; Hachicha, M.; Kammoun, T.; Bouaaziz, A.; Amri, F.; Harbi, A.; Zribi, M.; Bousnina, S.; Khemakhem, A.; Boujaafar, N.; Trabelsi, Abdelhalim; Steele, A. Duncan

doi:10.1016/j.patbio.2009.05.007

Cited by 14 publications

(1 citation statement)

References 42 publications

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“…Statistical analysis was done using SPSS version 25 and P values ≤ 0.05 were considered significant. Primers used for VP7 genotyping of human rotavirus strains [19].…”

Section: Methodsmentioning

confidence: 99%

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2021

World J. Biol. Pharm. Res.

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Aim: To explore the prevalence of rotavirus infection along with the molecular detection and genotyping of group A rotavirus (RVA) among bovine calves up to 5 months old in Diyala province-Iraq.Methods: This is a cross sectional study conducted in Diyala province-Iraq during the period of 2019-2020. One hundred bovine calves with age range of 1-5 months were included in the study. All were suffering acute gastroenteritis. Serum anti-rotavirus IgM and IgG plus fecal rotavirus Ag were tested for using ELISA techniques. Stool samples positive for rotavirus Ag were submitted for reverse transcription PCR (RT-PCR) for G and P genes, followed by sequencing and genotyping thereafter. Statistical analysis was done using SPSS version 25 and P values ≤ 0.05 were considered significant. Results:The positivity rate of anti-rotavirus IgM was 80% (P = 0.0001), and that of anti-rotavirus IgG was 79% (P = 0.0001). The rotavirus stool antigen was detected in 68% of calves (P = 0.01). A total of 45 stool samples which were positive for rotavirus Ag were submitted for RT-PCR; 13 (28.9%) were positive and 32 (71.1%) were negative (P = 0.084). 10 PCR positive samples were used for sequencing and genotyping and indicated that all investigated strains belonged to G1P[8] genotype. Conclusion:The current strains analyzed belonged to the G1P[8] RVA genotypes, affirming that employment of VP7 gene polymorphism accurately yielded uniform phylogenetic distances amongst investigated rotavirus strains and that there were no noticeable assortment events between human and animal rotavirus strains in Diyala province.

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“…Statistical analysis was done using SPSS version 25 and P values ≤ 0.05 were considered significant. Primers used for VP7 genotyping of human rotavirus strains [19].…”

Section: Methodsmentioning

confidence: 99%

Untitled

2021

World J. Biol. Pharm. Res.

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Circulation of group A rotaviruses among neonates of human, cow and pig: study from Assam, a north eastern state of India

et al. 2013

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Rotavirus (RV) infections are worldwide in distribution causing high morbidity and mortality in human and animal neonates. Human settlements in close proximity of animals aids for genetic re-assortment of the virus by interspecies transmission and consequent emergence of new viral antigenic strain. Therefore, the present study was designed to explore RV incidence in a single approach from human and animal neonates sharing similar environment. Altogether, 200 diarrheal samples from children (50), piglets (80) and calves (70) were collected during the year of 2010-2012 from various locality, farms and hospitals, initially screened through monoclonal antibody based enzyme immunoassay followed by RNA-PAGE and VP7 gene amplification by Reverse transcription PCR. The overall prevalence of rotavirus was found to be 41.5 % (83/200) where maximum numbers of positive cases were found in piglets (46.3 %) followed by human (40 %) and cow (37.1 %). Majority of samples demonstrated characteristic group A rotavirus (RVA) electropherotype of 4:2:3:2 pattern. Moreover, RNA profiles of seven samples from piglets and calves revealed variation in the migration pattern of class II, III and class IV segments. The study, for the first time from the valley, detected 43.7 % of neonatal RVA positive cases from human and animal sharing similar setting. The variation in RNA migration pattern in seven cases signifies tentative cases of gene re-assortment that warrant further evaluation.

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