Evidence from field nitrogen balance and 15N natural abundance data for the contribution of biological N2 fixation to Brazilian sugarcane varieties

Urquiaga, Segundo; Rathinam, Xavier; Morais, Rafael Fiusa de; Batista, Rafael B.; Schultz, Nivaldo; Leite, José Marcos; Sá, Jerusa Maia e; Barbosa, Karolina P.; Resende, Alexander Silva de; Alves, B. J. R.; Boddey, Robert M.

doi:10.1007/s11104-011-1016-3

Cited by 149 publications

(118 citation statements)

References 36 publications

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“…This study found indication of BNF (δ 15 N value significant at p <0.05, compared to the average value of reference plants) in varieties RB863129, RB867515, RB931011 and RB962962. The Ndda% values did not exceed 20%, a proportion of fixed N below the values reported for other sugarcane varieties (Lima et al, 1987;Urquiaga et al, 1992Urquiaga et al, , 2012Baptista et al, 2014). The differences in δ 15 N between reference species (castor bean and cotton) and sugarcane were small, ranging from 1.17 % for variety …”

Section: Discussionmentioning

confidence: 54%

The 15N natural abundance technique to assess the potential of biological nitrogen fixation (BNF) in some important C4 grasses

Carvalho¹,

Menezes²,

Freitas³

et al. 2017

Aust J Crop Sci

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Grasses such as sugarcane (Saccharum spp.), maize (Zea mays L.) and sorghum (Sorghum bicolor L. Moench) with C4 photosynthetic system are important crops that can host endophytic bacteria capable for biological nitrogen fixation (BNF). However, fixation measurements have yielded conflicting results. We determined BNF of five sugarcane and two sorghum varieties, maize and Guinea (Panicum maximum Jacq), elephant (Pennisetum purpureum Schum) and buffel (Cenchrus ciliaris L.) grasses in a pot experiment, using the 15 N natural abundance technique, with a soil naturally rich in 15 N (δ 15 N > 16 ‰) and two reference species (castor bean, Ricinus communis L., and cotton, Gossypium hirsutum L.) that absorb only N from the soil (average δ 15 N = 9.2 ‰). The initial results showed that no fixation occurred in elephant and buffel grasses. Sorghum, maize, Guinea grass and four sugarcane varieties had δ 15 N signals significantly lower than both reference species, indicating that they absorbed N from the atmosphere (δ 15 N = 0 ‰). The differences in signals from the reference species translates into proportions of plant N content originating from BNF varied from 12.8 to 19.4 % for sugarcane, 22.4% for maize, 20.9% for Guinea grass, and 24.7 to 31.2 % for sorghum. Nitrogen fixation by these species represents a potential of great fertilizer economy and high yields in low input agriculture.

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Section: Discussionmentioning

confidence: 54%

The 15N natural abundance technique to assess the potential of biological nitrogen fixation (BNF) in some important C4 grasses

Carvalho¹,

Menezes²,

Freitas³

et al. 2017

Aust J Crop Sci

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“…This indicates that native soil N played an important role for sugarcane yield. In addition, the possibility of a contribution of biological nitrogen fixation (BNF) to the plants that may reach up to 40 kg N•ha −1 •year −1 (Urquiaga et al 2012) cannot be neglected.…”

Section: Nitrogen Use Efficiencymentioning

confidence: 99%

“…Nitrogen is usually a limiting nutrient to crop yield in the weathered tropical soils, and positive yield responses to N fertilization are observed for sugarcane in Brazil (Dourado Neto et al 2010;Fortes et al 2013), although less frequently to plant cane (first cycle) owing to higher contribution from N in soil and residue due to N mineralization, as well as the biological nitrogen fixation (Balasubramanian et al 2004;Urquiaga et al 2012;Otto et al 2016). A review on sugarcane pointed out the relatively low N use efficiency (NUE) by the crop, which averages 26% from a range of 7 to 40% of the N fertilizer applied.…”

Section: Introductionmentioning

confidence: 99%

Agronomic performance of green cane fertilized with ammonium sulfate in a coastal tableland soil

et al. 2017

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“…Northeast region is the oldest one cropped with sugarcane that was brought by the Portuguese as stems pieces at the time they colonized Brazil. Since then, new cultivars were developed and crop productivity increased almost 30% in the last 30 years, but the lowest response of sugarcane to nitrogen fertilizer remained critical, especially in the first year of cultivation (Urquiaga et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Several studies report that diazotrophic bacteria promote a significant development in sugarcane (Oliveira et al, 2006;Schultz et al, 2012;Schultz et al, 2014). Urquiaga et al (2012) estimated the contribution of biological nitrogen fixation (BNF) in nine varieties using two different methods and found that at least 40 kg N ha -1 per year derived from this biological process, although the contribution rate differed according to the tested genotype. These studies showed how plant genotype response oscillates towards the association with diazotrophic bacteria, indicating that further studies are needed for better understanding of the plant-bacteria interaction.…”

Section: Introductionmentioning

confidence: 99%

Diazotrophic bacteria associated to sugarcane varieties cropped at Northeast Region of Brazil

Antonio

Rouws

Teixeira

et al. 2016

Agraria

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The objective of this work was to study the diversity of diazotrophic bacteria associated to 10 varieties of sugarcane cultivated in two states of the Northeast of Brazil and to identify the isolates by morphological and molecular methods. Diversity was studied by the application of traditional growth-depending microbiological methods: Gram-staining, comparison of colony morphology and growth on several culture media. Phylogenetic taxonomy was performed using 16S rRNA gene sequencing data. Almost all isolates obtained in semi-solid nitrogen free media were classified as Gram-negative and seven colony morphological groups were distinguished after growth on Potato medium. Among the 10 varieties evaluated, RB72454 resulted in higher number of isolates and diversity. The great majority of isolates from most of the genotypes tested showed similar characteristic to the genus Gluconacetobacter based on its peculiar colony morphology and growth characteristics in semi-solid media. Comparison of the 16S rRNA sequence of each isolate to the Genbank showed that they are closely related to Gluconacetobacter, Burkholderia, Kosakonia, Klebsiella and Stenotrophomonas.Key words: biological nitrogen fixation, ecology of diazotrophic bacteria, sugarcane Bactérias diazotróficas associadas a variedades de cana-de-açúcar cultivadas na região Nordeste do Brasil RESUMOO objetivo deste trabalho foi estudar a diversidade de bactérias diazotróficas associadas a 10 variedades de cana cultivadas em dois estados do Nordeste do Brasil e identificar os isolados por métodos morfológicos e moleculares. A diversidade foi estudada através da aplicação de métodos microbiológicos tradicionais dependentes de crescimento: coloração de Gram, comparação da morfologia de colónias e crescimento em vários meios de cultura. Taxonomia filogenética foi realizada utilizando dados de sequenciamento de gene 16S rRNA. Quase todos os isolados obtidos em meios semissólidos livres de nitrogênio foram classificados como Gram-negativos e sete grupos baseados na morfologia de colônias foram distinguidos após crescimento em meio batata. Entre as 10 variedades avaliadas, RB72454 resultou em maior diversidade e número de isolados. A grande maioria dos isolados dos genótipos testados apresentaram característica similar ao gênero Gluconacetobacter com base em seu peculiar crescimento em meios semissólidos e morfologia da colônia. A comparação da sequência do 16S rRNA de cada isolado com sequencias do GenBank mostrou que eles estão intimamente relacionados com Gluconacetobacter,

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Evidence from field nitrogen balance and 15N natural abundance data for the contribution of biological N2 fixation to Brazilian sugarcane varieties

Cited by 149 publications

References 36 publications

The 15N natural abundance technique to assess the potential of biological nitrogen fixation (BNF) in some important C4 grasses

The 15N natural abundance technique to assess the potential of biological nitrogen fixation (BNF) in some important C4 grasses

Agronomic performance of green cane fertilized with ammonium sulfate in a coastal tableland soil

Diazotrophic bacteria associated to sugarcane varieties cropped at Northeast Region of Brazil

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