volume 55, issue 5, P1389-1395 1990
DOI: 10.1135/cccc19901389
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Abstract: Endopolygalacturonase (E.C. 3.2.1.15) of Aspergillus niger was modified with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and glycine ethyl ester. The modification resulted in total irreversible inactivation of the enzyme and derivatization of carboxyl acid residues and tyrosine residues. The treatment of the modified enzyme with hydroxylamine led to a restoration of modified tyrosine residues but not to reactivation of the enzyme. The inactivation with carbodiimide was pH dependent, the rate of inactivation …

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