Evidence for the interaction of the regulatory protein Ki-1/57 with p53 and its interacting proteins

Nery, Flávia C.; Rui, Edmilson; Kuniyoshi, Taís M.; Kobarg, Jörg

doi:10.1016/j.bbrc.2006.01.036

Cited by 25 publications

(55 citation statements)

References 43 publications

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“…Two other domains are related to the RNA recognition: motif domain and Hyaluronan/mRNA-binding protein. The latter is involved in nuclear functions, such as the remodeling of chromatin and the regulation of transcription [56,57]. Another important conserved domain is methyltransferase type 11.…”

Section: Resultsmentioning

confidence: 99%

Sugarcane transcriptome analysis in response to infection caused by Acidovorax avenae subsp. avenae

et al. 2016

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Sugarcane is an important tropical crop mainly cultivated to produce ethanol and sugar. Crop productivity is negatively affected by Acidovorax avenae subsp avenae (Aaa), which causes the red stripe disease. Little is known about the molecular mechanisms triggered in response to the infection. We have investigated the molecular mechanism activated in sugarcane using a RNA-seq approach. We have produced a de novo transcriptome assembly (TR7) from sugarcane RNA-seq libraries submitted to drought and infection with Aaa. Together, these libraries present 247 million of raw reads and resulted in 168,767 reference transcripts. Mapping in TR7 of reads obtained from infected libraries, revealed 798 differentially expressed transcripts, of which 723 were annotated, corresponding to 467 genes. GO and KEGG enrichment analysis showed that several metabolic pathways, such as code for proteins response to stress, metabolism of carbohydrates, processes of transcription and translation of proteins, amino acid metabolism and biosynthesis of secondary metabolites were significantly regulated in sugarcane. Differential analysis revealed that genes in the biosynthetic pathways of ET and JA PRRs, oxidative burst genes, NBS-LRR genes, cell wall fortification genes, SAR induced genes and pathogenesis-related genes (PR) were upregulated. In addition, 20 genes were validated by RT-qPCR. Together, these data contribute to a better understanding of the molecular mechanisms triggered by the Aaa in sugarcane and opens the opportunity for the development of molecular markers associated with disease tolerance in breeding programs.

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Section: Resultsmentioning

confidence: 99%

Sugarcane transcriptome analysis in response to infection caused by Acidovorax avenae subsp. avenae

et al. 2016

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“…Although we have demonstrated a role for HMG2L1 in attenuating expression of smooth muscle differentiation genes, this does not preclude possible additional roles in regulating SMC proliferation. Previous studies have shown HMGB1 regulates P53-mediated transcription (27) and HMG2L1 is a potential P53 binding protein (28). Given that P53 is a critical transcription factor involved in cell cycle arrest and activation of apoptotic program (29), it will be very important to assess whether HMG2L1 is also involved in apoptosis or cell cycle regulation in SMCs.…”

Section: Discussionmentioning

confidence: 99%

Repression of Smooth Muscle Differentiation by a Novel High Mobility Group Box-containing Protein, HMG2L1

Zhou

Wang

2010

Journal of Biological Chemistry

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The molecular mechanisms regulating smooth muscle-specific gene expression during smooth muscle development are poorly understood. Myocardin is an extraordinarily powerful cofactor of serum response factor (SRF) that stimulates expression of smooth muscle-specific genes. In an effort to search for proteins that regulate myocardin function, we identified a novel HMG box-containing protein HMG2L1 (high mobility group 2 like 1). We found that HMG2L1 expression is correlated with the smooth muscle cell (SMC) synthetic phenotype. Overexpression of HMG2L1 in SMCs down-regulated smooth muscle marker expression. Conversely, depletion of endogenous HMG2L1 in SMCs increases smooth muscle-specific gene expression. Furthermore, we found HMG2L1 specifically abrogates myocardin-induced activation of smooth muscle-specific genes. By GST pulldown assays, the interaction domains between HMG2L1 and myocardin were mapped to the N termini of each of the proteins. Finally, we demonstrated that HMG2L1 abrogates myocardin function through disrupting its binding to SRF and abolishing SRF-myocardin complex binding to the promoters of smooth muscle-specific genes. This study provides the first evidence of this novel HMG2L1 molecule playing an important role in attenuating smooth muscle differentiation.

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“…By analyzing our protein-protein interaction data we realized that many of the Ki-1/57 partners had already been described to directly bind to p53 or others members of the p53 family (e.g., p73). 4 All of these proteins are functionally interconnected with each other and are direct or indirectly involved with regulatory mechanisms of transcription (Fig. 1).…”

Section: From Interaction To Functionmentioning

confidence: 99%

From protein interaction profile to functional assignment: The human protein Ki-1/57 is associated with pre-mRNA splicing events

Bressan

Kobarg²

2010

RNA Biology

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Evidence for the interaction of the regulatory protein Ki-1/57 with p53 and its interacting proteins

Cited by 25 publications

References 43 publications

Sugarcane transcriptome analysis in response to infection caused by Acidovorax avenae subsp. avenae

Sugarcane transcriptome analysis in response to infection caused by Acidovorax avenae subsp. avenae

Repression of Smooth Muscle Differentiation by a Novel High Mobility Group Box-containing Protein, HMG2L1

From protein interaction profile to functional assignment: The human protein Ki-1/57 is associated with pre-mRNA splicing events

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