Evidence for oxylipin synthesis and induction of a new polyunsaturated fatty acid hydroxylase activity in Chondrus crispus in response to methyljasmonate

Gaquerel, Emmanuel; Hervé, Cécile; Labriere, Christophe; Boyen, Catherine; Potin, Philippe; Salaün, Jean‐Pierre

doi:10.1016/j.bbalip.2007.02.007

Cited by 37 publications

(35 citation statements)

References 44 publications

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“…Interestingly, only two genes encoding lipoxygenase (SI Appendix, Table S7.10) have been identified, a surprising result given the diversity of oxylipins observed in this alga. The presence of methyl jasmonate, a plant hormone involved in stress signaling, has been detected in vitro after incubation with linolenic acid (37,38). However, no candidates for allene oxide synthase, allene oxide cyclase, or jasmonic acid carboxyl methyltransferase were found.…”

Section: Recent Genome Expansion Resulting From Transposable Elementmentioning

confidence: 99%

Genome structure and metabolic features in the red seaweed Chondrus crispus shed light on evolution of the Archaeplastida

Collén

Porcel

Carré

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Red seaweeds are key components of coastal ecosystems and are economically important as food and as a source of gelling agents, but their genes and genomes have received little attention. Here we report the sequencing of the 105-Mbp genome of the florideophyte Chondrus crispus (Irish moss) and the annotation of the 9,606 genes. The genome features an unusual structure characterized by gene-dense regions surrounded by repeat-rich regions dominated by transposable elements. Despite its fairly large size, this genome shows features typical of compact genomes, e.g., on average only 0.3 introns per gene, short introns, low median distance between genes, small gene families, and no indication of large-scale genome duplication. The genome also gives insights into the metabolism of marine red algae and adaptations to the marine environment, including genes related to halogen metabolism, oxylipins, and multicellularity (microRNA processing and transcription factors). Particularly interesting are features related to carbohydrate metabolism, which include a minimalistic gene set for starch biosynthesis, the presence of cellulose synthases acquired before the primary endosymbiosis showing the polyphyly of cellulose synthesis in Archaeplastida, and cellulases absent in terrestrial plants as well as the occurrence of a mannosylglycerate synthase potentially originating from a marine bacterium. To explain the observations on genome structure and gene content, we propose an evolutionary scenario involving an ancestral red alga that was driven by early ecological forces to lose genes, introns, and intergenetic DNA; this loss was followed by an expansion of genome size as a consequence of activity of transposable elements.T he red algae, together with the glaucophytes and the Chloroplastida, are members of the Archaeplastida, the phylogenetic group formed during the primary endosymbiosis event that gave rise to the first photosynthetic eukaryote. Red algal genomes, both plastid and nuclear, also contributed, via secondary endosymbiosis, to several other eukaryotic lineages, including

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Section: Recent Genome Expansion Resulting From Transposable Elementmentioning

confidence: 99%

Genome structure and metabolic features in the red seaweed Chondrus crispus shed light on evolution of the Archaeplastida

Collén

Porcel

Carré

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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317

297

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“…Other than that, all reports of cyclopentenones reported from seaweeds originate from red, not brown algae -e.g., methyl jasmonate in Chondrus crispus (Bouarab et al 2004), PGA 2 , PGE 2 , PGF 2 and 15-keto-prostaglandins in Gracilaria verrucosa (Sajiki 1997, Imbs et al 2001, Dang et al 2010 and PGE 2 in Gracilaria tenuistipitata (Hsu et al 2008) and G. rhodocaudata (Sajiki 1997). In none of these cases, a function could be associated with these compounds yet, even though in several cases prostaglandin production was found to be upregulated by some form of stress (e.g., Sajiki 1997, Imbs et al 2001, Bouarab et al 2004, Gaquerel et al 2007, Hsu et al 2008. Interestingly, prostaglandin content (or production, respectively) has been associated with occasional cases of toxicity of seaweeds to humans (Higa and Kuniyoshi 2000).…”

Section: Laminaria Digitatamentioning

confidence: 99%

Prostaglandin A₂triggers a strong oxidative burst in Laminaria: a novel defense inducer in brown algae?

Zambounis¹,

Gaquerel²,

Strittmatter³

et al. 2012

ALGAE

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We report an oxidative burst triggered by prostaglandin A 2 (PGA 2 ) in the brown algal kelp Laminaria digitata, constituting the first such discovery in an alga and the second finding of an oxidative burst triggered by a prostaglandin in a living organism. The response is more powerful than the oxidative burst triggered by most other chemical elicitors in Laminaria. Also, it is dose-dependent and cannot be inhibited by diphenylene iodonium, suggesting that another source than NAD(P)H oxidase is operational in the production of reactive oxygen species. Despite the very strong oxidative response, rather few effects at other levels of signal transduction pathways could be identified. PGA 2 does not increase lipolysis (free fatty acids) in Laminaria, and only one oxylipin (15-hydroxyeicosatetraenoic acid; 15-HETE) was found to be upregulated in Laminaria. In a subsequent set of experiments in the genome model Ectocarpus siliculosus, none of 5 selected candidate genes, all established participants in various stress responses, showed any significant differences in their expression profiles.Key Words: Cyclopentenone; diphenylene iodonium; Phaeophyta; polyunsaturated fatty acids; prostaglandin A 2 INTRODUCTIONBrown algae (Phaeophyta) make up most of the benthic biomass on temperate and polar rocky shores. Kelp forests are key ecosystems of such coastal areas and they constitute habitat and breeding grounds for a very large diversity of marine life forms -besides offering a physical protection by providing a wave energy-absorbing buffer for coasts. Brown algae belong to a lineage that has been evolving independently of other major photosynthetic Received December 20, 2011, Accepted February 23, 2012 *Corresponding Author E-mail: fkuepper@abdn.ac.uk Tel: +44-1224+44- -274490, Fax: +44-1224 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. (Reymond and Farmer 1998, Orozco-Cárdenas et al. 2001).The oxidative burst was initially discovered in human macrophages (Baldridge and Gerard 1933). It is now recognized also as a key element of plant (Wojtaszek 1997, Mittler et al. 2004) and algal , Dring 2005, Cosse et al. 2007) defense, where it is considered both as a rapid defense response and an internal emergency signal. It consists of the rapid and massive production of ROS such as superoxide (O 2 -), hydrogen peroxide (H 2 O 2 ), and hydroxyl radicals (• OH)which are likely produced via the interaction of plasma membrane-associated NAD(P)H oxidases (Wojtaszek 1997). Hence, pathogen attack triggers both the production of ROS and the oxidation cascade of fatty acids leading to the production of bioactive oxylipins in plants. In algal systems, the oxidative burst was initially discovered in response to injury in a red alga (Collén et al. 1994). Over the last decade, oxidative...

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“…Pathogen attacks are known to induce the de-novo formation of oxylipins, which are generated by oxygenation of PUFA. Oxylipins form part of the defense mechanism against negative effects of endophytic pathogens in C. crispus [50][51][52]. This aspect might further contribute to the here observed differences in the fatty composition between C. crispus and M. stellatus.…”

Section: Discussionmentioning

confidence: 78%

Fatty acid compositions associated with high-light tolerance in the intertidal rhodophytes Mastocarpus stellatus and Chondrus crispus

et al. 2017

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The rhodophytes Mastocarpus stellatus and Chondrus crispus occupy the lower intertidal zone of rocky shores along North Atlantic coastlines, with C. crispus generally occurring slightly deeper. Consequently, M. stellatus is exposed to more variable environmental conditions, related to a generally higher stress tolerance of this species. In order to extend our understanding of seasonal modulation of stress tolerance, we subjected local populations of M. stellatus and C. crispus from Helgoland, North Sea, to short-term high-light stress experiments over the course of a year (October 2011, March, May and August 2012). Biochemical analyses (pigments, antioxidants, total lipids, fatty acid compositions) allowed to reveal mechanisms behind modulated high-light tolerances. Overall, C. crispus was particularly more susceptible to high-light at higher water temperatures (October 2011 and August 2012). Furthermore, speciesspecific differences in antioxidants, total lipid levels and the shorter-chain/longer-chain fatty acid ratio (C14 + C16/ C18 + C20) were detected, which may enhance the tolerance to high-light and other abiotic stress factors in M. stellatus, so that this species is more competitive in the highly variable upper intertidal zone compared to C. crispus. Since the high-light tolerance in C. crispus seemed to be affected by water temperature, interactions between both species may be impacted in the future by rising mean annual sea surface temperature around the island of Helgoland.

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Evidence for oxylipin synthesis and induction of a new polyunsaturated fatty acid hydroxylase activity in Chondrus crispus in response to methyljasmonate

Cited by 37 publications

References 44 publications

Genome structure and metabolic features in the red seaweed Chondrus crispus shed light on evolution of the Archaeplastida

Genome structure and metabolic features in the red seaweed Chondrus crispus shed light on evolution of the Archaeplastida

Prostaglandin A₂triggers a strong oxidative burst in Laminaria: a novel defense inducer in brown algae?

Fatty acid compositions associated with high-light tolerance in the intertidal rhodophytes Mastocarpus stellatus and Chondrus crispus

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Evidence for oxylipin synthesis and induction of a new polyunsaturated fatty acid hydroxylase activity in Chondrus crispus in response to methyljasmonate

Cited by 37 publications

References 44 publications

Genome structure and metabolic features in the red seaweed Chondrus crispus shed light on evolution of the Archaeplastida

Genome structure and metabolic features in the red seaweed Chondrus crispus shed light on evolution of the Archaeplastida

Prostaglandin A2triggers a strong oxidative burst in Laminaria: a novel defense inducer in brown algae?

Fatty acid compositions associated with high-light tolerance in the intertidal rhodophytes Mastocarpus stellatus and Chondrus crispus

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Prostaglandin A₂triggers a strong oxidative burst in Laminaria: a novel defense inducer in brown algae?