Evidence for Functional Conservation, Sufficiency, and Proteolytic Processing of the CLAVATA3 CLE Domain

Ni, Jun; Clark, Steven E.

doi:10.1104/pp.105.072678

Cited by 151 publications

(199 citation statements)

References 24 publications

(23 reference statements)

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“…b1b2b3c1 plants are phenotypically stronger than clv3 alleles in Col-0, suggesting that multiple CLV3-related ligands might contribute to CLV clade signaling and SAM function in Arabidopsis, as they do in rice (Suzaki et al, 2009). Consistent with this hypothesis, several CLV3-related CLE genes can substitute for loss of CLV3 when expressed from the CLV3 promoter (Ni and Clark, 2006). BAM receptor gene repression represents a specific transcriptional output as a consequence of the CLV1 signaling pathway in RM cells, which is not observed in the homeodomain transcription factor phb phv cna mutants.…”

Section: Discussionsupporting

confidence: 66%

Plant stem cell maintenance by transcriptional cross-regulation of related receptor kinases

et al. 2015

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The CLAVATA3 (CLV3)-CLAVATA1 (CLV1) ligand-receptor kinase pair negatively regulates shoot stem cell proliferation in plants. clv1 null mutants are weaker in phenotype than clv3 mutants, but the clv1 null phenotype is enhanced by mutations in the related receptor kinases BARELY ANY MERISTEM 1, 2 and 3 (BAM1, 2 and 3). The basis of this genetic redundancy is unknown. Here, we demonstrate that the apparent redundancy in the CLV1 clade is in fact due to the transcriptional repression of BAM genes by CLV1 signaling. CLV1 signaling in the rib meristem (RM) of the shoot apical meristem is necessary and sufficient for stem cell regulation. CLV3-CLV1 signaling in the RM represses BAM expression in wild-type Arabidopsis plants. In clv1 mutants, ectopic BAM expression in the RM partially complements the loss of CLV1. BAM regulation by CLV1 is distinct from CLV1 regulation of WUSCHEL, a proposed CLV1 target gene. In addition, quadruple receptor mutants are stronger in phenotype than clv3, pointing to the existence of additional CLV1/BAM ligands. These data provide an explanation for the genetic redundancy seen in the CLV1 clade and reveal a novel feedback operating in the control of plant stem cells.

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Section: Discussionsupporting

confidence: 66%

Plant stem cell maintenance by transcriptional cross-regulation of related receptor kinases

et al. 2015

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“…CLV1 encodes a receptorlike kinase containing leucine-rich repeat (LRR) motifs, CLV2 a LRR receptor-like protein and CLV3 a small secreted polypeptide (Clark et al, 1997;Fletcher et al, 1999;Jeong et al, 1999;Rojo et al, 2002;Ni and Clark, 2006). Recent findings have shown that transient inactivation of the CLV pathway leads to rapid alterations in the expression of meristem regulators, consistent with earlier studies investigating the effect of clv mutations on WUS expression (Reddy and Meyerowitz, 2005).…”

Section: Introductionsupporting

confidence: 74%

POL and PLL1 phosphatases are CLAVATA1 signaling intermediates required forArabidopsisshoot and floral stem cells

2006

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The post-embryonic development of above-ground tissues in plants is dependent upon the maintenance and differentiation of stem cells at the shoot meristem. The Arabidopsis WUSCHEL (WUS) transcription factor establishes an organizing center within the shoot meristem that is essential for specification of stem-cell identity in overlying cells. The CLAVATA (CLV) signaling pathway, including the CLV1 receptor-kinase, promotes the differentiation of stem cells by limiting the WUS expression domain, yet the mechanism of CLV signaling is largely unknown. Previously, we have shown that mutations in two protein phosphatases, POLTERGEIST (POL) and PLL1, partially suppress clv mutant phenotypes. Here, we demonstrate that POL and PLL1 are integral components of the CLV1 signaling pathway. POL and PLL1 are essential for stem-cell specification, and can also block stem-cell differentiation when overexpressed. We provide extensive evidence that POL and PLL1 act downstream of CLV signaling to maintain WUS expression and that they regulate WUS at a transcriptional level. Our findings suggest that POL and PLL1 are central players in regulating the balance between stem-cell maintenance and differentiation, and are the closest known factors to WUS regulation in the shoot meristem.

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“…Studies on the putative CLE peptides of Arabidopsis (Ito et al, 2006;Ni and Clark, 2006;Strabala et al, 2006;Whitford et al, 2008) have shown a direct relationship between CLE domain sequence and induced phenotypes. MtCLE12 and MtCLE13 are most similar to a group of less characterized Arabidopsis CLE peptides (AtCLE1-AtCLE7) that are broadly produced with higher activity levels in the root (Sharma et al, 2003;Ito et al, 2006).…”

Section: Discussion Cle Family In M Truncatulamentioning

confidence: 99%

CLE Peptides Control Medicago truncatula Nodulation Locally and Systemically

Mortier

Herder²,

Whitford³

et al. 2010

Plant Physiology

297

409

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The CLAVATA3/embryo-surrounding region (CLE) peptides control the fine balance between proliferation and differentiation in plant development. We studied the role of CLE peptides during indeterminate nodule development and identified 25 MtCLE peptide genes in the Medicago truncatula genome, of which two genes, MtCLE12 and MtCLE13, had nodulation-related expression patterns that were linked to proliferation and differentiation. MtCLE13 expression was up-regulated early in nodule development. A high-to-low expression gradient radiated from the inner toward the outer cortical cell layers in a region defining the incipient nodule. At later stages, MtCLE12 and MtCLE13 were expressed in differentiating nodules and in the apical part of mature, elongated nodules. Functional analysis revealed a putative role for MtCLE12 and MtCLE13 in autoregulation of nodulation, a mechanism that controls the number of nodules and involves systemic signals mediated by a leucine-rich repeat receptor-like kinase, SUNN, which is active in the shoot. When MtCLE12 and MtCLE13 were ectopically expressed in transgenic roots, nodulation was abolished at the level of the nodulation factor signal transduction, and this inhibition involved long-distance signaling. In addition, composite plants with roots ectopically expressing MtCLE12 or MtCLE13 had elongated petioles. This systemic effect was not observed in transgenic roots ectopically expressing MtCLE12 and MtCLE13 in a sunn-1 mutant background, although nodulation was still strongly reduced. These results suggest multiple roles for CLE signaling in nodulation.

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Evidence for Functional Conservation, Sufficiency, and Proteolytic Processing of the CLAVATA3 CLE Domain

Cited by 151 publications

References 24 publications

Plant stem cell maintenance by transcriptional cross-regulation of related receptor kinases

Plant stem cell maintenance by transcriptional cross-regulation of related receptor kinases

POL and PLL1 phosphatases are CLAVATA1 signaling intermediates required forArabidopsisshoot and floral stem cells

CLE Peptides Control Medicago truncatula Nodulation Locally and Systemically

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