Evidence for a functional glycogen metabolism in mature mammalian spermatozoa

Utilization of tyrosine‐ and histidine‐containing dipeptides to enhance productivity and culture viability

“…Sertoli cells showed higher levels of MGS than germ cells pool, suggesting important differences between somatic cells and male germ cells respect to glycogen metabolism. In contrast to previously published results by Ballester et al [2000], we detected the muscle isoform of GS but not the liver isoform in mammalian spermatozoa. In addition, we confirmed the presence of MGS in spermatozoa from human, stallion, boar, dog, rat, and mouse using immunofluorescence (data not shown).…”

Metabolic strategy of boar spermatozoa revealed by a metabolomic characterization

“…However, despite PDH activity, no fatty acid synthesis from glucose took place. The absence of net glycogen synthesis, low G6P levels, and CO 2 production rate of boar sperm contrast with the high G6P levels, glycogen synthesis, and CO 2 production previously reported in dog [9,11,23]. Results obtained here for boar sperm together with previously reported results in dog suggest that different metabolic profiles are associated with these two main types of mammalian sperm cells.…”

An integrated overview on the regulation of sperm metabolism (glycolysis-Krebs cycle-oxidative phosphorylation)

“…As previously described in this manuscript, evidence of oxidation of lactate to pyruvate has been detected in horse spermatozoa, and also in pig spermatozoa, in which oxidation of external lactate is inhibited in the presence of the MCT inhibitor α-cyano-4-hydroxicinnamate and by the LDH oxamate inhibitor, indicating that lactate is transported into sperm mitochondria where it is oxidized to pyruvate (Gladden, 2004;Brooks, 2018). Results from different studies indicate gluconeogenesis-linked glycogen metabolism is present in spermatozoa (Ballester et al, 2000;Palomo et al, 2003;Albarracin et al, 2004). In this model lactate is incorporated by mature spermatozoa, and converted into glycogen; in dogs, at least, this is considered to have a major function in providing energy for capacitation.…”