Evaluation of wound healing and anti-inflammatory activity of the leaves of Calpurnia aurea (Ait.) Benth (fabaceae) in mice

Ayal, Getachew; Belay, Assefa; Kahaliw, Wubayehu

doi:10.1016/j.wndm.2019.100151

Cited by 31 publications

(33 citation statements)

References 49 publications

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“…ey were acclimatized to the laboratory condition for a week prior to the experiment. Animals were handled according to international laboratory animal use and care guidelines throughout the experiment [21].…”

Section: Acute Oral Toxicitymentioning

confidence: 99%

Antidiarrheal Activity of Hydromethanolic Root Extract and Solvent Fractions of Clutia abyssinica Jaub. & Spach. (Euphorbiaceae) in Mice

Zayede

Mulaw

Kahaliw

2020

Evidence-Based Complementary and Alternative Medicine

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Introduction. Diarrheal diseases are associated with an estimated 1.3 million deaths annually, with most occurring in resource-limited countries; up to 25% of deaths in young children living in Africa and southeast Asia are attributable to acute gastroenteritis. Due to limitations associated with various treatments available, the need for developing newer drugs is imperative. Objective. This study was aimed to evaluate the antidiarrheal activity of root extract and fractions of C. abyssinica Jaub. & Spach. (Euphorbiaceae) in mice. Methods. After plant extraction and subsequent fractionation of the crude extract, the antidiarrheal activity was screened in castor oil induced diarrhea, castor oil induced enteropooling, and gastrointestinal motility test models accordingly. Result. The root extract of C. abyssinica produced neither visible signs of toxicity nor death at a single dose of 2000 mg/kg, suggesting the LD50 > 2000 mg/kg. In the castor oil induced diarrheal model, the highest dose of the extract (400 mg/kg) showed a maximal inhibition in the onset (158.00 ± 14.64, p<0.01, in minutes) of wet feces as compared to the negative control. In the enteropooling model, 400 mg/kg treated mice showed a significant reduction in volume (0.47 ± 0.02 ml, p<0.01) and weight (0.50 ± 0.02 g, p<0.05) of intestinal content as compared to the vehicle treated group. In the gastrointestinal motility test, the hydromethanolic root extract of C. abyssinica significantly inhibited the intestinal transit of charcoal meal at 400 mg/kg. In addition, chloroform and n-butanol fractions significantly reduced the distance moved by charcoal at doses of 200 mg/kg and 400 mg/kg, whereas aqueous fraction showed a significant effect at all test doses. The highest antidiarrheal index was observed at the maximal dose of extract and each fraction. Conclusion. The results obtained showed that the findings provide scientific support for the folkloric repute of C. abyssinica roots as treatment of diarrhea.

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Section: Acute Oral Toxicitymentioning

confidence: 99%

Antidiarrheal Activity of Hydromethanolic Root Extract and Solvent Fractions of Clutia abyssinica Jaub. & Spach. (Euphorbiaceae) in Mice

Zayede

Mulaw

Kahaliw

2020

Evidence-Based Complementary and Alternative Medicine

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“…is antibacterial activity may contribute to the wound healing effect by keeping the wound free of infections and complications. Such types of agents contribute to the rapid healing of wound [29,42]. e period of epithelialization was reduced from 18.67 days (simple ointment) to 14.67, 12.67, and 13 days for 5% CEO-, 10% CEO-, and NF-treated groups, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…Epithelialization Period Measurement. Complete epithelization period was calculated as the number of days required for falling off of the dead tissue remnants without any residual raw wound [28,29].…”

Section: Excision Wound Modelmentioning

confidence: 99%

“…It was measured through continuous water flow technique by considering the gram of water required to break the skin (Figure 2(b)). Tensile strength was calculated using the following formula [29,31]:…”

Section: Linear Incision Wound Modelmentioning

confidence: 99%

“…An increase in paw volume at 1, 2, 3, and 4 h after carrageenan injection was considered as the parameter for measurement of inflammation. e average foot swelling in extract treated mice as well as standard was compared with that of the negative control, and the percent inhibition (anti-inflammatory activity) of edema was determined using the following formula [29,34,35]:…”

Section: Linear Incision Wound Modelmentioning

confidence: 99%

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In Vivo Evaluation of Wound Healing and Anti‐Inflammatory Activity of 80% Methanol Crude Flower Extract of Hagenia abyssinica (Bruce) J.F. Gmel in Mice

Belachew

Asrade

Geta

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Background. Hagenia abyssinica (Bruce) J.F. Gmel (Rosaceae) is distributed in the highlands of Ethiopia. The flowers of Hagenia abyssinica (Bruce) J.F. Gmel are used traditionally to treat wound. However, there was no scientific report on wound healing activity of Hagenia abyssinica (Bruce) J.F. Gmel. Thus, this study was initiated to investigate the wound healing and anti-inflammatory activities of 80% methanol crude extract of flowers of Hagenia abyssinica in mice. Objective. The objective of this study was to evaluate the wound healing and anti-inflammatory activity of 80% methanol crude flower extract of Hagenia abyssinica in mice. Methods. Air-dried flowers of Hagenia abyssinica were grounded and macerated three times successively by 80% methanol. The dried extract was fractionated with chloroform, ethyl acetate, and water. Phytochemical screening tests were performed according to established procedures. The crude extract and solvent fractions were formulated as ointments. Wound healing activity of the crude extract was evaluated using excision and incision wound models, and the wound healing activities of solvent fractions were evaluated by using the excision wound model. The anti-inflammatory activity of the 80% methanol extract of Hagenia abyssinica was evaluated using carrageenan-induced hind paw edema model in mice. Result. The 2000 mg/kg test dose of the 10% (w/w) crude extract ointment was safe in rats. Both the 5% (w/w) and 10% (w/w) crude extract ointment-treated groups showed significant wound contraction starting from the day 4th. Both 5% (w/w) and 10% (w/w) crude extract ointments showed significant (P<0.001) increment of tensile strength compared to the negative control. The 10% (w/w) aqueous and ethyl acetate fraction ointment revealed high (P<0.001) percentage of wound contraction. The 100 mg/kg, 200 mg/kg, and 400 mg/kg oral administration of the crude extract had significant inhibition of the paw edema in mice of carrageenan-induced inflammation. Conclusion. The results of this study evidenced that both 5% w/w and 10% w/w 80% methanol extract ointment of the flowers of Hagenia abyssinica have wound healing and anti-inflammatory effects.

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Anti-inflammatory Activity of Medicinal Plants: Present Status and Future Perspectives

Chouhan

Guleria

2020

Botanical Leads for Drug Discovery

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Evaluation of wound healing and anti-inflammatory activity of the leaves of Calpurnia aurea (Ait.) Benth (fabaceae) in mice

Cited by 31 publications

References 49 publications

Antidiarrheal Activity of Hydromethanolic Root Extract and Solvent Fractions of Clutia abyssinica Jaub. & Spach. (Euphorbiaceae) in Mice

Antidiarrheal Activity of Hydromethanolic Root Extract and Solvent Fractions of Clutia abyssinica Jaub. & Spach. (Euphorbiaceae) in Mice

In Vivo Evaluation of Wound Healing and Anti‐Inflammatory Activity of 80% Methanol Crude Flower Extract of Hagenia abyssinica (Bruce) J.F. Gmel in Mice

Anti-inflammatory Activity of Medicinal Plants: Present Status and Future Perspectives

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