Evaluation of Two Different Homogeneous Assays for LDL-Cholesterol in Lipoprotein-X-positive Serum

Hua, Fei; Maeda, Shin; Kirii, Hirokazu; Fujigaki, Suwako; Maekawa, Naoya; Fujii, Hidehiko; Wada, Hisayasu; Seishima, Mitsuru

doi:10.1093/clinchem/46.9.1351

Cited by 37 publications

(11 citation statements)

References 18 publications

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“…The method has been extensively evaluated and compared with β-quantification and in multicenter evaluation (59), in samples of normolipidemic subjects (58,60,61), in patients with hyperlipidemia (58,(60)(61)(62), and in nonfasting samples (59,63). The method is not completely specific for LDL, since IDL is only partially recovered (64)(65)(66) and apoE-rich HDL and VLDL are not completely excluded (64)(65), the latter especially in diabetic patients (67), whereas results for Lp(a) are contradictory (58,66,68). It is also reported that small, dense LDL are partially recovered (66).…”

Section: Friedewald Equationmentioning

confidence: 99%

Evaluation of Methods for the Measurement of Low-Density Lipoprotein Cholesterol

Bairaktari

Seferiadis

Elisaf

2005

J Cardiovasc Pharmacol Ther

111

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A high concentration of low-density lipoprotein cholesterol (LDL-C) in plasma is one of the strongest risk factors for atherosclerotic cardiovascular disease and mortality. The most common approach to determining LDL-C in the clinical laboratory is the Friedewald calculation. There is an increased interest to improve the accuracy of LDL-C estimated by this equation. The expert panel convened by National Cholesterol Education Program has recommended the development of accurate direct methods to measure LDL-C. Several homogeneous and fully automated methods have been introduced in recent years that show improved precision and accuracy over earlier methods, especially the Friedewald calculation. Each of the atherogenic particles in plasma—very-low, intermediate-, and low-density lipoprotein—as well as lipoprotein (a), contain one molecule of apolipoprotein B (apoB) and thus, plasma total concentration of apoB reflects the number of atherogenic particles. Several studies suggested that the measurement of apoB could improve the prediction of risk of coronary artery disease. Thus, in addition to the newly developed direct assays, alternative calculation procedures have been proposed that also take into consideration total serum apoB concentration for the estimation of LDL-C and the presence of small, dense LDL particles. The new generation of homogenous methods for the measurement of LDL-C and the use of serum apoB concentration for the estimation of LDL-C can contribute to the accurate LDL-C determination.

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Section: Friedewald Equationmentioning

confidence: 99%

Evaluation of Methods for the Measurement of Low-Density Lipoprotein Cholesterol

Bairaktari

Seferiadis

Elisaf

2005

J Cardiovasc Pharmacol Ther

111

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“…Em consequência dessa diferença caracterizada, a decisão a respeito da conduta terapêutica pode ser inadequada, dependendo das metodologias e dos equipamentos utilizados. Estudos cujas publicações datam desde 1998 demonstram que considerável variabilidade analítica de LDL-C pode ser verificada em decorrência da utilização de metodologias baseadas em princípios analíticos distintos (5,9,8,28,31,33,38) .…”

Section: Discussionunclassified

Variabilidade interensaios de dislipidemias em pacientes hipertensos

Rosini¹,

Rosini²,

Rosini³

et al. 2009

J. Bras. Patol. Med. Lab.

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Introdução e objetivos: Ensaios de diferentes procedências para avaliação das dislipidemia podem resultar em variações significativas nos resultados obtidos e consequente conduta inadequada pelo clínico. O estudo objetivou comparar resultados laboratoriais de colesterol total (CT), triglicérides (TG), colesterol da lipoproteína de alta densidade (HDL-C), colesterol da lipoproteína de baixa densidade (LDL-C), apolipoproteína A-1 (Apo A-1), apolipoproteína B (Apo B) e lipoproteína (a) (Lp[a]) e índices lipídicos (não-HDL-C, CT/HDL-C, LDL-C/HDL-C, TG/HDL-C e Apo B/HDL-C) de pacientes hipertensos e/ou diabéticos diagnosticados. Métodos: Foram utilizados conjuntos reativos, e os respectivos analisadores Gold Analisa, Dia Sys (CCX-Abbott), Dade Behring (Nefelômetro BN 100) e Roche (COBAS Integra 400), para verificar a reprodutibilidade dos resultados obtidos. Participaram 99 pacientes (36 do sexo masculino e 63 do feminino). Comparando os resultados, verificamos que: todas as médias obtidas dos constituintes lipídicos apresentaram diferença significativa; número semelhante de pacientes apresentou níveis séricos elevados de CT, TG, Lp(a) e Apo A-1. O HDL-C, o LDL-C e a Apo B apresentaram discordância, assim como os índices de CT/LDL-C, LDL-C/HDL-C e TG/HDL-C. Para não-HDL-C e ApoB/HDL, houve semelhança no número de pacientes com valores não recomendados. Em consequência da diferença, em relação ao LDL-C, a decisão da conduta terapêutica poderá ser inadequada, enquanto o não-HDL-C, além de evidenciar partículas aterogênicas, apresentou número de hipertensos com valores séricos não referendados semelhantes, independente da metodologia e do equipamento utilizado. Conclusão: No grupo de hipertensos analisados, o não-HDL-C se caracterizou um importante fator de correção interensaios de parâmetros lipídicos. E sua associação à relação Apo B/HDL-C pode ser um fator adicional em relação às condutas hipolipemiantes a serem adotadas.

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“…5 Therefore accurate and precise measurements of subjects LDL-C concentrations are necessary to appropriately identify individuals with hypercholesterolemia and to monitor the response of various treatments as well as lifestyle changes. 6 The LDLs are spherical particles, having a hydrophobic oily core of cholesteryl ester and triglycerides (TG). These particles have a hydrophilic envelop of phospholipids, free cholesterol and apolipoproteins.…”

Section: Introductionmentioning

confidence: 99%

A comparative analysis of direct LDL-C assay and Friedewald’s formula in subjects of ischemic heart disease & stroke in a tertiary care centre

Chatterjee¹,

Sendhav²,

Kakaiya³

et al. 2020

IJCBR

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Introduction: Among the various risk factors for the development of Ischemic heart diseases (IHD) as well as stroke is elevated low density lipoprotein cholesterol (LDL-C) levels. This makes accurate reporting of LDL-C crucial in the management of IHD. On one hand estimation of LDL-C by direct method is available, but it is expensive and on the other hand the Friedewald's formula is most commonly employed but a method with limitations. Aim: Therefore the aim of this study is to evaluate the correlation between calculated LDL-C with direct LDL-C method and analyse their appropriateness. Materials and Methods: This study was a hospital based cross-sectional study. LDL-C was measured by both Friedewald's formula (FF) and direct LDL method in 501 participants between the age group of 30-70 years. Paired t-test was used to test the difference in LDL concentration obtained by a direct method and Friedewald's formula. The level of significance was taken as P < 0.05. Results: The mean and standard deviation (SD) of LDL-C estimated by both direct assay and FF showed a significant difference (p<0.05) at TG ranges of <150 and 151-399 mg/ dL respectively but there is no significant difference (p >0.05) at TG range >400 mg/dL. There was a discrepancy of 37 out of 501participants (7.38%) classified as cardiac risk groups by the two methods used. A significant correlation between LDL-C levels obtained by FF and direct methods, (p value <0.05, r value= 0.8101, CI = 0.7777 to 0.8382) was observed. Conclusion:The study concludes that both methods can be employed depending on TG levels. Direct LDL estimation appears to be unreliable at TG concentrations >400 mg/dL but useful when sample is collected from a non-fasting participants.

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Evaluation of Two Different Homogeneous Assays for LDL-Cholesterol in Lipoprotein-X-positive Serum

Cited by 37 publications

References 18 publications

Evaluation of Methods for the Measurement of Low-Density Lipoprotein Cholesterol

Evaluation of Methods for the Measurement of Low-Density Lipoprotein Cholesterol

Variabilidade interensaios de dislipidemias em pacientes hipertensos

A comparative analysis of direct LDL-C assay and Friedewald’s formula in subjects of ischemic heart disease & stroke in a tertiary care centre

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