Evaluation of Two Commercial Systems and a New Identification Scheme Based on Solid Substrates for Identifying Coagulase‐Negative Staphylococci from Bovine Mastitis

Thorberg, B.-M.; Brändström, Boel

doi:10.1046/j.1439-0450.2000.00399.x

Cited by 20 publications

(18 citation statements)

References 28 publications

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“…The tube coagulate test was determined and evaluated for coagulation after 3 and 24 h of incubation. All isolates identified as NSA and CNS based on colony morphology, Gram staining (positive), catalase reaction (positive), and coagulase test (positive) were subjected to species identification by using all the conventional biochemical methods described by Thorberg and Brandstrom (2000). For novobiocin-sensitive CNS isolates identified in samples, a simplified identification system, novobiocin-sensitive CNS built on biochemical tests was used (Thorberg and Brandstrom, 2000).…”

Section: Methodsmentioning

confidence: 99%

“…All isolates identified as NSA and CNS based on colony morphology, Gram staining (positive), catalase reaction (positive), and coagulase test (positive) were subjected to species identification by using all the conventional biochemical methods described by Thorberg and Brandstrom (2000). For novobiocin-sensitive CNS isolates identified in samples, a simplified identification system, novobiocin-sensitive CNS built on biochemical tests was used (Thorberg and Brandstrom, 2000). To confirm the isolates as Staphylococcus, definitive identification of all strains included in the study was performed targeting the Staphylococcus genus-specific 16S rRNA gene polymerase chain reaction (fragment of 756 bp); F: AAC TCT GTT ATT AGG GAA GAA CA and R: CCA CCT TCC TCC GGT TTG TCA CC as previously described (Zhang et al, 2004).…”

Section: Methodsmentioning

confidence: 99%

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Prevalence and Antimicrobial Resistance Profile of Staphylococcus Species in Chicken and Beef Raw Meat in Egypt

Osman

Amer

Badr³

et al. 2015

Foodborne Pathogens and Disease

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Coagulase-positive (CPS) and coagulase-negative (CNS) staphylococci cause staphylococcal food poisoning. Recently, CPS and CNS have received increasing attention due to their potential role in the dissemination of antibiotic resistance markers. The present study aimed to evaluate CPS and CNS species distribution and their antibiotic resistance profile isolated from chicken and beef meat. Fifty fresh, uncooked chicken parts and 50 beef meat cuts (local n=27; imported n=23) were used. One hundred staphylococcal isolates belonging to 11 species were isolated and identified from chicken (n=50) and beef (n=50) raw meat samples. Staphylococcus hyicus (26/100), lugdunensis (18/100), aureus (15/100) and epidermidis (14/100) were dominant. S. aureus was 100% resistant to penicillin and sulfamethoxazole/trimethoprim. Vancomycin-resistant S. aureus showed intermediate resistance (51%), which might indicate the dissemination of vancomycin resistance in the community and imply food safety hazards. The percentage of resistance to β-lactams was variable, with the highest resistance being to penicillin (94%) and lowest to ampicillin-sulbactam (22%). Antimicrobial resistance was mainly against penicillin (94%), clindamycin (90%) and sulfamethoxazole/trimethoprim (82%). The results indicate that chicken and beef raw meat are an important source of antibiotic-resistant CPS and CNS.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Prevalence and Antimicrobial Resistance Profile of Staphylococcus Species in Chicken and Beef Raw Meat in Egypt

Osman

Amer

Badr³

et al. 2015

Foodborne Pathogens and Disease

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“…The inoculation of Peptone agar (P agar) supplemented with 7 mg of acriflavine per ml was conducted according to [37]. Coagulase-negative staphylococci (CNS) were identified according to [36] with 2 modifications: (1) a commercially available substrate tablet (Rosco, Taastrup, Denmark) was used to test β -galactosidase activity; and (2) the acetone test was performed as described by [28]. …”

Section: Methodsmentioning

confidence: 99%

Occurrence of Clinical and Sub-Clinical Mastitis in Dairy Herds in the West Littoral Region in Uruguay

Gianneechini

Concha

Rivero³

et al. 2002

Acta Vet Scand

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-Twenty-nine dairy farms were selected to determine the incidence of clinical mastitis, prevalence of sub-clinical mastitis and bacterial aetiology in the West Littoral Region of Uruguay. In samples taken by the owner and frozen at -20 ºC during a week the incidence rate of clinical mastitis was determined as 1.2 cases per 100 cow-months at risk. Staphylococcus aureus was the most common isolated pathogen in 37.5% of 40 milk samples from clinical cases obtained in 1 month. No bacteria grew in the 32.5% of the total samples. A sub-sample including 1077 dairy cows from randomly selected farms was used to determine the prevalence of sub-clinical mastitis. These samples were taken on one visit to each farm. The prevalence was 52.4% on a cow basis and 26.7% on an udder quarter basis. In 55.1% of the quarters of the selected animals with more than 300 000 cells/ml there was no growth. The isolated pathogens from sub-clinical cases and their relative frequencies were: Staphylococcus aureus 62.8%, Streptococcus agalactiae 11.3%, Enterococcus sp. 8%, coagulase-negative staphylococci 7.4%, Streptococus uberis 6.4%, Streptococcus dysgalactiae 1.8%, Escherichia coli 1.5% and Staphylococcus hyicus coagulase-positive 0.6%.

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“…Moreover, miniaturized panels of biochemical tests are considered expensive and are not accurate for the identification of Staphylococcus spp. in samples collected from animals (16,17). Molecular biology techniques offer advantages due to their more rapid speed and specificity for the identification of microorganisms (18).…”

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confidence: 99%

Identification of Coagulase-Negative Staphylococci from Bovine Intramammary Infection by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

et al. 2014

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bCoagulase-negative staphylococci (CoNS) are among the main pathogens causing bovine intramammary infection (IMI) in many countries. However, one of the limitations related to the specific diagnosis of CoNS is the lack of an accurate, rapid, and convenient method that can differentiate the bacterial species comprising this group. The aim of this study was to evaluate the ability of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to accurately identify CoNS species in dairy cow IMI. In addition, the study aimed to determine the frequency of CoNS species causing bovine IMI. A total of 108 bacterial isolates were diagnosed as CoNS by microbiological cultures from two milk samples collected from 21 dairy herds; the first sample was collected at the cow level (i.e., 1,242 composite samples from all quarters), while the second sample was collected at the mammary quarter level (i.e., 1,140 mammary samples collected from 285 cows). After CoNS isolation was confirmed by microbiological culture for both samples, all CoNS isolates (n ‫؍‬ 108) were genotypically differentiated by PCR restriction fragment length polymorphism (RFLP) analysis of a partial groEL gene sequence and subjected to the MALDI-TOF MS identification procedure. MALDI-TOF MS correctly identified 103 (95.4%) of the CoNS isolates identified by PCR-RFLP at the species level. Eleven CoNS species isolated from bovine IMI were identified by PCR-RFLP, and the most prevalent species was Staphylococcus chromogenes (n ‫؍‬ 80; 74.1%). In conclusion, MALDI-TOF MS may be a reliable alternative method for differentiating CoNS species causing bovine IMI.

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Evaluation of Two Commercial Systems and a New Identification Scheme Based on Solid Substrates for Identifying Coagulase‐Negative Staphylococci from Bovine Mastitis

Cited by 20 publications

References 28 publications

Prevalence and Antimicrobial Resistance Profile of Staphylococcus Species in Chicken and Beef Raw Meat in Egypt

Prevalence and Antimicrobial Resistance Profile of Staphylococcus Species in Chicken and Beef Raw Meat in Egypt

Occurrence of Clinical and Sub-Clinical Mastitis in Dairy Herds in the West Littoral Region in Uruguay

Identification of Coagulase-Negative Staphylococci from Bovine Intramammary Infection by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

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