Evaluation of Trypanocidal and Antioxidant Activities of a Selected Series of 3-amidocoumarins

Moncada‐Basualto, Mauricio; Lapier, Michel; Maya, Juan Diego; Matsuhiro, Betty; Olea‐Azar, Claudio; Delogu, Giovanna; Uriarte, Eugenio; Santana, Lourdes; Matos, María Joäo

doi:10.2174/1573406414666180419113437

Cited by 9 publications

(7 citation statements)

References 41 publications

(67 reference statements)

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“…The antioxidant activity was lower for AgNPs-Sm, which is consistent with that for ORAC-FL. Moncada-Basualto et al 62 determined that cellular antioxidant activity has a relationship with lipophilicity (ie, major lipophilicity activity is associated with minor antioxidant activity), possibly due to a better interaction with the cell membrane, which could indicate the low percentages of antioxidant activity of the extracts and AgNPs-Sm, as well as the limited entry of the compounds into the cells, due to the polarity of the aqueous extracts. The main polyphenolic compounds in Solanum are quercetin, gallic acid, and rutin, among others.…”

Section: Resultsmentioning

confidence: 99%

“…Cellular antioxidant activity (CAA) was evaluated in VERO cell line culture (European Collection of Cell Cultures, ECACC 84113001) using 2´,7´-dichlorodihydrofluorescein diacetate (DCFH 2 -DA) as a fluorescent probe. 62 The cells were plated in sterile, white, polystyrene, flat-bottom 96-well microplates (Nunc, Denmark) at a concentration of 50,000 cells per well and incubated for 24 h at 37 °C and 5% CO 2 in RPMI 1640 culture medium. The cells were washed with 150 mL of phosphate buffered saline (PBS) pH 7.4 and incubated for 1 h with 100 µL of RPMI 1640 containing 20 µM.…”

Section: Methodsmentioning

confidence: 99%

“…Antioxidant capacity was determined using the ORACfluorescence methodology (ORAC-FL). [61][62][63][64][65] The analyses were performed using a 96-well white polystyrene plate reader (EnSpire multimode PerkinElmer, Waltham, MA, USA). The reaction was carried out in phosphate buffer of 75 mM (pH 7.4).…”

Section: Total Antioxidant Activity By Oxygen Radical Absorbance Capacity (Orac-fl)mentioning

confidence: 99%

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Determination of Antioxidant Activity by Oxygen Radical Absorbance Capacity (ORAC-FL), Cellular Antioxidant Activity (CAA), Electrochemical and Microbiological Analyses of Silver Nanoparticles Using the Aqueous Leaf Extract of Solanum mammosum L.

Pilaquinga¹,

Morey²,

Fernández³

et al. 2021

IJN

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Purpose The importance of studying polyphenolic compounds as natural antioxidants has encouraged the search for new methods of analysis that are quick and simple. The synthesis of silver nanoparticles (AgNPs) using plant extracts has been presented as an alternative to determine the total polyphenolic content and its antioxidant activity. Methods In this study, aqueous leaf extract of Solanum mammosum , a species of plant endemic to South America, was used to produce AgNPs. The technique of oxygen radical absorption capacity using fluorescein (ORAC-FL) was used to measure antioxidant activity. The oxidation of the 2´,7´-dichlorodihydrofluorescein diacetate (DCFH 2 -DA) as fluorescent probe was used to measure cellular antioxidant activity (CAA). Electrochemical behavior was also examined using differential pulse voltammetry (DPV) and cyclic voltammetry (CV). Total polyphenolic content (TPH) was analyzed using the Folin-Ciocalteu method, and the major polyphenolic compound was analyzed by high performance liquid chromatography with diode array detector (HPLC/DAD). Finally, a microbial analysis was conducted using Escherichia coli and Bacillus sp. Results The average size of nanoparticles was 5.2 ± 2.3 nm measured by high-resolution transmission electron microscopy (HR-TEM). The antioxidant activity measured by ORAC-FL in the extract and nanoparticles were 3944 ± 112 and 637.5 ± 14.8 µM ET/g of sample, respectively. Cellular antioxidant activity was 14.7 ± 0.2 for the aqueous extract and 12.5 ± 0.2 for the nanoparticles. The electrochemical index (EI) was 402 μA/V for the extract and 324 μA/V for the nanoparticles. Total polyphenolic content was 826.6 ± 20.9 and 139.7 ± 20.9 mg EGA/100 g of sample. Gallic acid was the main polyphenolic compound present in the leaf extract. Microbiological analysis revealed that although leaf extract was not toxic for Escherichia coli and Bacillus sp., minor toxic activity for AgNPs was detected for both strains. Conclusion It is concluded that the aqueous extract of the leaves of S. mammosum contains nontoxic antioxidant compounds capable of producing AgNPs. The methods using AgNPs can be used as a fast analytical tool to monitor the presence of water-soluble polyphenolic compounds from plant origin. Analysis and detection of new antioxidants from plant extracts may be potentially applicable in biomedicine.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Total Antioxidant Activity By Oxygen Radical Absorbance Capacity (Orac-fl)mentioning

confidence: 99%

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Determination of Antioxidant Activity by Oxygen Radical Absorbance Capacity (ORAC-FL), Cellular Antioxidant Activity (CAA), Electrochemical and Microbiological Analyses of Silver Nanoparticles Using the Aqueous Leaf Extract of Solanum mammosum L.

Pilaquinga¹,

Morey²,

Fernández³

et al. 2021

IJN

Self Cite

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“…The inhibition capacity was expressed as concentration Trolox equivalents (TE) per 100 g of dry mass, and this was quantified by integration of the area under the fluorescence decay curve (AUC). All reaction mixtures were prepared in triplicate, and at least three independent assays were performed for each sample [ 36 , 52 ].…”

Section: Methodsmentioning

confidence: 99%

“…Then, 50 μL of DMPO (5,5-dimethyl-1-pyrroline-N-oxide, 30 mM final concentration), 50 μL of the sample (20 mM in solvent used in extraction) and 50 μL of hydrogen peroxide (30%) were sequentially added. The mixture was put into an ESR cell, and the spectrum was recorded after five minutes of reaction [ 36 , 52 , 53 ].…”

Section: Methodsmentioning

confidence: 99%

Polyphenolic Composition and Antioxidant Activity (ORAC, EPR and Cellular) of Different Extracts of Argylia radiata Vitroplants and Natural Roots

et al. 2022

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Plant biochemistry studies have increased in recent years due to their potential to improve human health. Argylia radiata is an extremophile plant with an interesting polyphenolic profile. However, its biomass is scarce and occasionally available. Argylia in vitro biomass was obtained from tissue culture and compared with in vivo roots regarding its polyphenolic and flavonoid content. Different solvents were used to prepare extracts from the in vitro tissue of callus and aerial plant organs and in vivo roots. UPLC-MS/MS was used to assess the chemical composition of each extract. ORAC-FL and scavenging of free radicals (DPPH and OH) methods were used to determine the antioxidant capacity of extracts. Furthermore, the biological activity of the extracts was established using the cellular antioxidant activity method. The vitroplants were a good source of polyphenols (25–68 mg GAE/100 g tissue FW), and methanol was the most efficient solvent. Eight polyphenolic compounds were identified, and their antioxidant properties were investigated by different chemical methods with EPR demonstrating its specific scavenging activity against free radicals. All extracts showed cellular dose-dependent antioxidant activity. The methanolic extract of vitroplants showed the highest cellular antioxidant activity (44.6% and 51%) at 1 and 10 µg/mL of extract, respectively. Vitroplants of A. radiata are proposed as a biotechnological product as a source of antioxidant compounds with multiple applications.

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Cytogenotoxicity assessment of 3‐(3,4‐dihydroxyphenyl)‐7,8‐dihydroxycoumarin on HepG2/C3A cells and leukocytes

Castanha

Almeida-Terassi

Guardado-Yordi

et al. 2022

J of Applied Toxicology

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3-(3,4-Dihydroxyphenyl)-7,8-dihydroxycoumarin is a newly synthesized coumarin derivative with a potent antioxidant effect. The aim of the present study is to investigate the safety of this compound, determining the in vitro cytotoxic and genotoxic in human peripheral blood mononuclear cells (PBMC) and in HepG2/C3A cells. Cell viability has been investigated by the trypan blue staining test and MTT assay and the genotoxicity by the comet assay and micronucleus test, using concentrations between 0.01 and 10 μg/ml. The compound proved to be noncytotoxic in both cell lines, at all tested concentrations, protecting the cells from the DNA damage. In addition, this molecule does not show clastogenic/aneugenic effects when performing the micronucleus test with cytokinesis blockade. Based on the obtained data, and the conditions of the experiments, we can conclude that the 3-(3,4-dihydroxyphenyl)-7,8-dihydroxycoumarin is a safe molecule up to a concentration of 10 μg/ml, which encourages further studies aiming to explore its potential as a drug candidate.

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Evaluation of Trypanocidal and Antioxidant Activities of a Selected Series of 3-amidocoumarins

Cited by 9 publications

References 41 publications

Determination of Antioxidant Activity by Oxygen Radical Absorbance Capacity (ORAC-FL), Cellular Antioxidant Activity (CAA), Electrochemical and Microbiological Analyses of Silver Nanoparticles Using the Aqueous Leaf Extract of Solanum mammosum L.

Determination of Antioxidant Activity by Oxygen Radical Absorbance Capacity (ORAC-FL), Cellular Antioxidant Activity (CAA), Electrochemical and Microbiological Analyses of Silver Nanoparticles Using the Aqueous Leaf Extract of Solanum mammosum L.

Polyphenolic Composition and Antioxidant Activity (ORAC, EPR and Cellular) of Different Extracts of Argylia radiata Vitroplants and Natural Roots

Cytogenotoxicity assessment of 3‐(3,4‐dihydroxyphenyl)‐7,8‐dihydroxycoumarin on HepG2/C3A cells and leukocytes

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