Evaluation of thiol disulfide balance in adolescents with vitamin B12 deficiency

Abstract

Background Vitamin B12 is an important vitamin for metabolism and affects many mechanisms in the body including neuronal migration, DNA synthesis, neurotransmitter synthesis, brain and cognitive development. Increased oxidative stress in the body leads to the damage of the child development, but also plays a crucial role in the pathogenesis of many diseases encountered in the childhood period. Our aim is to investigate whether or not B12 deficiency is associated with dynamic thiol/disulfide homeostasis in adolescent patients. Methods This is a case-controlled observational study consisting of 45 adolescent patients with vitamin b12 deficiency and a control group consisting of 45 healthy adolescent. Patients between 11 and 18 ages who applied to the outpatient clinic for the first time with one of the complaints of headache were selected due to their decreased school performance, dizziness, and fatigue. Hemogram, vitamin B12, homocysteine levels and oxidative stress parameters such as native and total thiol disulfide levels and ratios of disulfide/native thiol, disulfide/total thiol, and native thiol/total thiol were measured from the patients. Results Vitamin B12 level was found to be significantly lower in vitamin B12 deficiency group (p < 0.001). The serum disulfide level was found to be 27.5 ± 8.38 in the case group and 20.5 ± 8.36 in the control group (p < 0.001). In the multiple linear regression analysis, it was determined that the independent variables of native thiol, homocysteine and disulfide levels effected of vitamin B12 levels (p < 0.001, p < 0.001, p < 0.005 respectively; R2 = 0.62). Conclusion The results obtained in terms of the effect of vitamin B12 deficiency on oxidative stress in adolescents are remarkable. The increase in oxidative stress parameters in the patient group may also suggest that oxidative stress plays a vital role in vitamin B12 deficiency in adolescence.

Association between thiol-disulfide hemostasis and transient tachypnea of the newborn in late-preterm and term infants

“…As a result of physical, biochemical factors and diseases encountered in early childhood, especially in neonatal period, the body's own OS balance is disrupted and causes many complications and morbidity [ 12 , 13 ]. The important studies have been carried out recently regarding the effect of thiol disulfide homeostasis, a new marker of the oxidative system, on diseases in the pediatric period [ 6 , 8 , 9 , 13 ].…”

“…Plasma total oxidant status (TOS) levels (µmol H 2 O 2 eq. /L) are expressed by spectrophotometric measurement of the sample formed as a result of the oxidants in the blood [ 8 ], oxidizing the ferrous ion-o-dianisidine complex to an iron ion and strengthening this reaction with glycerol molecules. Plasma Total Antioxidant Status (TAS) levels (µmol Trolox eqv.…”

“…Plasma Total Antioxidant Status (TAS) levels (µmol Trolox eqv. /L) are also a method based on the principle of measuring ABTS + (2,2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) cation after the reaction of H 2 O 2 and ABTS described by Erel [ 8 , 11 ].…”

“…In the calculation of oxidative stress index, firstly the TAS unit was converted to mol/L and the OSI unit was obtained with TOS/TAS [ 8 ].…”

“…Thiols in the structure of important amino acids containing sulphur, such as methionine, which is involved in the structure of many enzymatic reactions and hormones in the body, are the primary competition point for oxygen radicals. Oxidation of thiol groups with oxygen radicals also forms reversible disulfide bonds [ 6 , 8 ]. The result of oxidation at the cellular level is the earliest findings of early protein oxidation.…”