Evaluation of the toxic effect of star fruit on serum biochemical parameters in rats

Abstract

The objective of the present study was to evaluate the toxic effect of Averrhoa carambola (star fruit) juice at different storage conditions in Sprague Dawley (SD) rats. Twenty female rats weighing 180 ± 20 g were randomly assigned into four groups with five rats per group (n = 5). First group served as the control group, fed with distilled water (vehicle). Second, third and fourth groups were orally treated with juice of A. carambola stored for 0, 1 and 3 h respectively for 14 days. Cage-side observations were done daily after each treatment. Body weight, food consumption and water intake were recorded on day-0, day-3, day-7 and day-14. All rats were fasted overnight prior to blood collection through cardiac puncture on day-15. The levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), urea and creatinine in blood serum were measured. Data were analyzed using Dunnett's test. From the results obtained, there was no lethality found and LD50 could not be determined. Increment of ALT levels (P<0.05) was reported in those rats treated with A. carambola juice stored for 3 h. On the basis of these results, we can conclude that A. carambola juice stored for 0 hand 1 h are safe to be consumed. However, juice stored for 3 h exerts toxic effect on rat liver at hepatocellular level.

Protective role of quercetin against manganese-induced injury in the liver, kidney, and lung; and hematological parameters in acute and subchronic rat models

“…Several serum enzymes are used as indicators or markers for hepatocellular injuries, such as ALT and AST. 36 Injured on liver release those cytosolic enzymes (ie, ALT and AST) in the blood cause elevation of their concentration in blood. 37 , 38 From liver-function tests, we found that serum ALT and AST were significantly increased in rats treated with Mn.…”

Acute and subacute toxicity of an ethanolic extract of Melandrii Herba in Crl:CD sprague dawley rats and cytotoxicity of the extract in vitro

“…Serum biochemistry is important for monitoring liver function in toxicology studies [ 16 – 18 ]. The highest doses of MHEE caused an increase of GLU, TP, and ALB levels in female rats.…”

In vivo Biocompatibility, Biodistribution and Therapeutic Efficiency of Titania Coated Upconversion Nanoparticles for Photodynamic Therapy of Solid Oral Cancers

“…ALP is present in the cells lining the hepatic biliary ducts, while ALT exists in the cytoplasm of hepatocytes. 32 Increase in the plasma ALT concentration indicates altered hepatocellular membrane permeability resulting in the leakage of the enzyme into the bloodstream, 33 perhaps due to the presence of the nanoparticles. This could eventually progress to hepatocellular damage/necrosis, hepatocyte proliferation or hepatocellular degeneration.…”