Evaluation of the Synergistic Effect of Amikacin with Cefotaxime against <i>Pseudomonas aeruginosa</i> and Its Biofilm Genes Expression

El-Demerdash, Azza S.; Bakry, Neveen R

doi:10.5772/intechopen.91146

Cited by 6 publications

(2 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On other hands the MIC of free solution for Escherichia coli is 0.5 µg/ml. The MICs for aqueous cefotaxime sodium agreed with published values [38][39][40][41][42].…”

Section: In Vitro Evaluation Of the Antibacterial Activity Of Cefotax...supporting

confidence: 84%

Cefotaxime Eluting Niosomes as A Novel Approach to Potentiate the Antibacterial Activity

Alhazza¹,

Al-Lami²,

AlShaheen³

et al. 2022

Journal of Pharmaceutical Negative Results

View full text Add to dashboard Cite

Multidrug resistance has resulted in hurtful infections on treatment with common antimicrobials and is associated with health threatening problems. Antibiotic abuse in health care systems contributes to global resistance. β-lactams are the predominantly used antibiotics, and the most often used is cefotaxime, therefore facing a resistance problem. Potent cephalosporin derivatives, β-lactamase inhibitors, prodrugs, and drug nano-carriers are approaches to cutback bacterial resistance. An example of a nanocarrier applied in field of antibacterial activity enhancement is niosomes. Niosomes are self-assembled vesicles made from non-ionic surfactants with cholesterol also additives included or omitted that can provide good features such as antibiotic shielding, cleavage proofing, controlled release, and specific targeting. Cefotaxime niosomes preparation done by Thin-Film Hydration method using Tween 40, span 60, stearylamine, and cholesterol. The dried film was moisturised with buffered solution of cefotaxime at 45-55°C then the final nanosized niosomal cefotaxime was generated through a probe sonication. A 27 formulas were prepared and identified by dynamic light scattering so that polydispersity index and the vesicular size are measured. The selected formula of cefotaxime niosomes (CN6) has as showed the lowermost values and a prominent vesicular structure of 106 nm diameter on Transmission electron microscope and compatibility on Fourier Transform Infrared System analysis. CN6 remained unchanged in diameter and Poly-dispersity index after 100 days of storage at 2 to-80 C and also onsubsequent freeze drying and reconstitution. Entrapment efficiency was quantified by the ultracentrifugation method and was discovered to be 93 %. Antibiotic delivery by cefotaxime-eluting niosomes has been shown to be successful with antibacterial potentiation of 160, 8, 640 and 40 times better than aqueous solution against Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa respectively which is obviously a considerable drop in the minimum inhibitory concentrations of the selected formula versus the aqueous cefotaxime solution and this was evaluated using Agar well diffusion technique.

show abstract

“…On other hands the MIC of free solution for Escherichia coli is 0.5 µg/ml. The MICs for aqueous cefotaxime sodium agreed with published values [38][39][40][41][42].…”

Section: In Vitro Evaluation Of the Antibacterial Activity Of Cefotax...supporting

confidence: 84%

Cefotaxime Eluting Niosomes as A Novel Approach to Potentiate the Antibacterial Activity

Alhazza¹,

Al-Lami²,

AlShaheen³

et al. 2022

Journal of Pharmaceutical Negative Results

View full text Add to dashboard Cite

show abstract

“…A suspension of the bacterial suspension was prepared to equal the turbidity of a 0.5 McFarland standard (1.5 × 108 colony forming units (CFU) ml−1). The results was determined as resistance, sensitive, and intermediate according to (CLSI, 2018).…”

Section: Phenotypic Characterization Of P Aeruginosamentioning

confidence: 99%

Evaluation of the Synergistic Antimicrobial Activity of Amikacin with Norfloxacin against Pseudomonas aeruginosa Isolated from Buffaloes Clinical Mastitis

Abdelkhalek¹,

Nagib²,

Elias³

et al. 2023

AAVS

View full text Add to dashboard Cite

Mastitis is a serious and economically problem commonly prevalent in most dairy cattle and buffaloes herds. Pseudomonas aeruginosa (P. aeruginosa) is opportunistic pathogens involved in veterinary disorders including clinical mastitis in buffaloes. This study aimed to investigate the antibiogram pattern and synergistic effect of amikacin and norfloxacin against resistant P. aeruginosa isolates from mastitis origin. In addition, detection of some virulence and antibiotics resistance genes. Two hundred buffaloes were examined and sixty mastitis milk samples were collected from clinical cases from the period from October 2021 until March 2022. The acute mastitis sings were assessed according to cardinal signs of inflammation and milk abnormalities. Out of two hundred buffaloes, sixty (30%) were diagnosed as clinical mastitis according to inflammatory signs and the culture results reveled only 5 (8.3%) were P. aeruginosa. Most of P. aeruginosa exhibited resistance to most antimicrobials classes. Meanwhile, the minimal inhibitory concentration (MIC) for amikacin and norfloxacin is significantly reduced from 64 µg/mL to 1 µg/mL and from 256 µg/mL to 8µg/mL respectively with frictional inhibitory concentration (FIC) index 0.25. Therefore, the FIC index recognized a synergistic activity between amikacin and norfloxacin against all P. areuginosa isolates. The mPCR was an efficient tool for detection of virulence genes (exoT, toxA, oprL, and isaI) at 152, 396, 504, 606 bp respectively. In addition, all the P. aeruginosa were found to carry the resistance genes (qnrS, qnrA, aadB). The combination of norfloxacin plus amikacin suppressed the resistance pattern P. aeruginosa isolates. Therefore, their combination showed synergistic bacterial potential antimicrobial activity in treatment of mastitis due to P. aeruginosa infection and help in reducing the resistance problem.

show abstract

An Alternative Approach Using Nano-garlic Emulsion and its Synergy with Antibiotics for Controlling Biofilm-Producing Multidrug-Resistant Salmonella in Chicken

El-Demerdash,

Orady,

Matter

et al. 2023

Indian J Microbiol

View full text Add to dashboard Cite

Surface-growing antibiotic-resistant pathogenic Salmonella is emerging as a global health challenge due to its high economic loss in the poultry industry. Their pathogenesis, increasing antimicrobial resistance, and biofilm formation make them challenging to treat with traditional therapy. The identification of antimicrobial herbal ingredients may provide valuable solutions to solve this problem. Therefore, our aim is to evaluate the potency of nano garlic as the alternative of choice against multidrug-resistant (MDR) Salmonella isolates using disc diffusion and microdilution assays. Then, checkerboard titration in trays was applied, and FIC was measured to identify the type of interaction between the two antimicrobials. A disc diffusion assay revealed that neomycin was the drug of choice. The range of nano garlic MIC was 12.5–25 μg/ml, while the neomycin MIC range was 32–64 μg/ml. The FIC index established a synergistic association between the two tested drugs in 85% of isolates. An experimental model was used including nano garlic and neomycin alone and in combination against Salmonella infection. The combination therapy significantly improved body productivity and inhibited biofilm formation by more than 50% down regulating the CsgBAD, motB, and sipA operons, which are responsible for curli fimbriae production and biofilm formation in Salmonella serotypes.

show abstract

Evaluation of the Synergistic Effect of Amikacin with Cefotaxime against Pseudomonas aeruginosa and Its Biofilm Genes Expression

Cited by 6 publications

References 54 publications

Cefotaxime Eluting Niosomes as A Novel Approach to Potentiate the Antibacterial Activity

Cefotaxime Eluting Niosomes as A Novel Approach to Potentiate the Antibacterial Activity

Evaluation of the Synergistic Antimicrobial Activity of Amikacin with Norfloxacin against Pseudomonas aeruginosa Isolated from Buffaloes Clinical Mastitis

An Alternative Approach Using Nano-garlic Emulsion and its Synergy with Antibiotics for Controlling Biofilm-Producing Multidrug-Resistant Salmonella in Chicken

Contact Info

Product

Resources

About