Evaluation of the Seeplex^® Meningitis ACE Detection Kit for the Detection of 12 Common Bacterial and Viral Pathogens of Acute Meningitis

Abstract

BackgroundBacterial meningitis is an infectious disease with high rates of mortality and high frequency of severe sequelae. Early identification of causative bacterial and viral pathogens is important for prompt and proper treatment of meningitis and for prevention of life-threatening clinical outcomes. In the present study, we evaluated the value of the Seeplex Meningitis ACE Detection kit (Seegene Inc., Korea), a newly developed multiplex PCR kit employing dual priming oligonucleotide methods, for diagnosing acute meningitis.MethodsAnalytical sensitivity of the kit was studied using reference strains for each pathogen targeted by the kit, while it's analytical specificity was studied using the human genome DNA and 58 clinically well-identified reference strains. For clinical validation experiment, we used 27 control cerebrospinal fluid (CSF) samples and 78 clinical CSF samples collected from patients at the time of diagnosis of acute meningitis.ResultsThe lower detection limits ranged from 101 copies/µL to 5×101 copies/µL for the 12 viral and bacterial pathogens targeted. No cross-reaction was observed. In the validation study, high detection rate of 56.4% was obtained. None of the control samples tested positive, i.e., false-positive results were absent.ConclusionsThe Seeplex Meningitis ACE Detection kit showed high sensitivity, specificity, and detection rate for the identification of pathogens in clinical CSF samples. This kit may be useful for rapid identification of important acute meningitis-causing pathogens.

Polymerase chain reaction to search for Herpes viruses in uveitic and healthy eyes: a South African perspective

“…Analysis of aqueous humor specimens has been proven to be a safe and useful adjunct to a detailed history and thorough clinical examination. 10 Collection of the sample was remarkably well tolerated and did not result in any complications in these two cohorts of patients. The median age of presentation for the uveitic group was 30.5 years and these patients are more inclined to have a non-syneritic vitreous body thereby increasing the likelihood of a 'dry tap' on needle aspiration.…”

Investigation of the Causative Agents of Meningitis By Real-Time PCR Method

“…PCR gibi amplifikasyon temelli yöntemlerle, viral nükleik asidin BOS'da saptanması, SSS enfeksiyonlarının tanısında önemli bir dönüm noktası olmuş ve böylece viral etkenleri belirlemedeki başarı %20-30'lardan, günümüzde %50-70'lere ulaşmıştır (8) . BOS'da PCR yöntemiyle etken arama, HSV, CMV ve enteroviral menenjit tanısı için önerilen tanı yöntemidir (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19) . HSV menenjitleri, viral menenjitlerin %1-3'ünü oluşturur.…”

“…Menenjit şüpheli hastaya, yatıştan ve BOS örneği alınmadan önce genellikle antibiyotik verildiği için, bakteriyel etkenin kültürde üretilme-si zorlaşmaktadır. Bu nedenle nükleik asit temelli testler bakteriyi belirlemede de önemlidir (16) . Bu çalışmada, hastanemiz Moleküler ünitesine, SSS enfeksiyonu ön tanısı düşünülerek gönderilen BOS örneklerinden "Multiplex real-time PCR" yönte-miyle, viral ve bakteriyel etkenlerin belirlenmesi, bu etkenlerin yaşa ve mevsimlere göre değerlendi-rilmesi amaçlandı.…”

The increasing application of multiplex nucleic acid detection tests to the diagnosis of syndromic infections

“…This announcement reflects the current move towards the development and commercialization of detection technologies for the diagnosis of infectious syndromes whether, enteric [1][2][3][4][5][6], respiratory [7][8][9][10][11][12][13], sexually transmitted [14], affecting the central nervous system [15] or causing sepsis [16,17]. Commercially available tests for the detection of specific pathogens associated with urinary tract infection, neonatal infection or infection in the immunocompromised host have also been developed by commercial diagnostic companies including Fast-track Diagnostics (Junglinster, Luxembourg), AusDiagnostics Pty Ltd (Sydney, Australia) and Seegene (Seoul, Korea).…”