Evaluation of the physiological value of porcine luteal cells isolated in various stages of the luteal phase: Tissue culture approach

Abstract

Porcine luteal cells were collected from corpora lutea in four different stages of the luteal phase and cultured as monolayers. Progesterone (P4) secretion was assayed using radioimmunoassays (Gregoraszczuk, 1991). Luteal cells cultured from porcine corpora lutea collected in the early luteal phase maintained steroidogenic capacity for 6 days in culture until the time comparable with midluteal corpora lutea. Luteal cells collected from mature and regressing corpora lutea did not dedifferentiate during 2 days of culture. After this time secretion of progesterone decreased to undetectable amounts characteristic of old corpora lutea. The regression in the culture progressed. The results demonstrate that the degree of the decline of progesterone depends on the type of corpus luteum, which is connected to particular time intervals of the luteal phase. Before starting experiments it is necessary to take into consideration the stage of the luteal phase from which the material is collected for culture. This study provides evidence that long term culture is useful for investigating a variety of aspects of luteal function only if cells are collected in the early luteal phase. Short term culture is suitable for investigation of cells collected from mid and late luteal phase. Regulation of luteal function is dependent on stage of the luteal phase.

In Vitro Effect of Leptin on Growth Hormone (GH)- and Insulin-Like Growth Factor-I (IGF-I)-stimulated Progesterone Secretion and Apoptosis in Developing and Mature Corpora Lutea of Pig Ovaries

“…The phase of the estrous cycle was d e t e r m i n e d a c c o r d i n g t o t h e e s t a b l i s h e d morphological criteria [10]. Luteal cells were obtained from pools of freshly excised newly forming corpora lutea (ELP; 1-3 days after ovulation) and mature (MLP; 10-14 days after ovulation).…”

Form of Supplemental Selenium Affects the Expression of mRNA Transcripts Encoding Selenoproteins, and Proteins Regulating Cholesterol Uptake, in the Corpus Luteum of Grazing Beef Cows

“…Steroidogenic cells were cultured with or without PGE 2 (1 and 50 µM/mL, Cayman Chemical Company, Ann Arbor, MI, USA), LH (1 and 10 IU/mL, NIH LH-S26), or hCG (10 and 50 ng/mL, Sigma Aldrich, St. Louis, MO, USA). Treatment concentrations (low and high for each agonist) were based upon previous reports [46][47][48][49][50][51][52]. All treatments and agonist-free control groups were performed in triplicate wells within each replicate.…”