Evaluation of the new system (umu-test) for the detection of environmental mutagens and carcinogens

Oda, Yoshimitsu; Nakamura, Seiichi; Oki, Iwashiro; Kato, Takao; Shinagawa, Hideo

doi:10.1016/0165-1161(85)90062-7

Cited by 593 publications

(378 citation statements)

References 20 publications

Supporting

Mentioning

372

Contrasting

Unclassified

Order By: Relevance

“…The genotoxic effects of concentrated samples were determined with the SOS/umu bioassay (ISO 13829, 2000;Oda et al, 1985) using Salmonella typhimurium strain TA 1535/pSK1002. The test strain was provided by the Osaka Prefectural Institute of Public Health, Japan.…”

Section: Umu Assaymentioning

confidence: 99%

Evaluation of wastewater reclamation technologies based on in vitro and in vivo bioassays

Cao

Yang

Zhang

et al. 2009

Science of The Total Environment

View full text Add to dashboard Cite

Section: Umu Assaymentioning

confidence: 99%

Evaluation of wastewater reclamation technologies based on in vitro and in vivo bioassays

Cao

Yang

Zhang

et al. 2009

Science of The Total Environment

View full text Add to dashboard Cite

“…1). [13,21]. In the present study, the test strain S. typhimurium TA1535/pSK1002 was pre-cultured in L-Broth medium in an incubator at 37 • C, 198 L exponentially growing bacteria were mixed with 2-L samples into each well of the 96-well microplate (Corning ® ).…”

Section: Sample Collection and Preparationmentioning

confidence: 99%

“…It has been widely used for evaluating genotoxic compounds and for screening of genotoxicity of water bodies [11][12][13]. In addition, indirect DNAdamaging agents that need activation to show their damaging effects were successfully determined by use of exogenous metabolizing enzymes (S9) [14,15].…”

Section: Introductionmentioning

confidence: 99%

Assessing of genotoxicity of 16 centralized source-waters in China by means of the SOS/umu assay and the micronucleus test: Initial identification of the potential genotoxicants by use of a GC/MS method and the QSAR Toolbox 3.0

Yan

Jiang²,

Na³

et al. 2014

Mutation Research/Genetic Toxicology and Environmental Mutagene

View full text Add to dashboard Cite

Only few studies were conducted to assess genotoxicity of centralized source waters in China and almost none of them dealt with the causal relationship between the genotoxic effect and genotoxicants. In this work, 16 centralized source waters in China were sampled from five river systems and genotoxicity of their organic extracts was assessed by use of the SOS/umu test for DNA-damaging effect and the miniaturized flow cytometry-based micronucleus (MN) test for chromosome-damaging effect. In addition, initial identification of potential genotoxicants for the six samples from the Yangtze River was done with a GC/MS method and the QSAR toolbox 3.0. The results demonstrate that eight samples showed both indirect and direct DNA-damaging effects, another four samples showed only indirect DNA-damaging effects, while chromosome-damaging effects were found for 14 out of the 16 samples, in which aneugenic and clastogenic modes of action were found for 4 and 10 samples, respectively. Both direct/indirect DNAdamaging effects and chromosome-damaging effects were induced by the six Yangtze River samples, and the existing different types of genotoxicant confirmed the results. Furthermore, o-phenylphenol was initially identified as the major cause for the DNA-damaging effects while PAHs, pesticides, phenol and anthraquinone were identified as ubiquitous chromosome-damaging agents among these samples. In conclusion, a combination of the SOS/umu test and the miniaturized flow cytometry-based MN test to detect both DNA-damaging and chromosome-damaging effects could be used as a comprehensive genotoxicity assessment tool for the evaluation and classification of genotoxicity of complex mixtures, and potential genotoxicants can be initially identified with additional information from chemical analysis and the QSAR toolbox.

show abstract

“…Among these bioassays, the umu/SOS assay, a sensitive in vitro method for genotoxicity screening, has been increasingly applied to rapid screening of mutagens and carcinogens in environmental samples [24][25][26][27]. As for endocrinedisrupting effects, some in vitro bioassays, such as estrogen screen (E-SCREEN), yeast estrogen screen (YES), and estrogen-responsive chemically activated luciferase, have been developed to evaluate integrated estrogenic activity of environmental samples [28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Assessment of hormonal activities and genotoxicity of industrial effluents using in vitro bioassays combined with chemical analysis

Fang

Ying

Zhao

et al. 2012

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Wastewaters from various industries are a main source of the contaminants in aquatic environments. The authors evaluated the hormonal activities (estrogenic/anti‐estrogenic activities, androgenic/anti‐androgenic activities) and genotoxicity of various effluents from textile and dyeing plants, electronic and electroplate factories, pulp and paper mills, fine chemical factories, and municipal wastewater treatment plants in the Pearl River Delta region by using in vitro bioassays (yeast estrogen screen [YES]; yeast androgen screen [YAS]; and genotoxicity assay [umu/SOS]) combined with chemical analysis. The results demonstrated the presence of estrogenic, anti‐estrogenic, and anti‐androgenic activity in most industrial effluents, whereas no androgenic activities were detected in all of the effluents. The measured estrogenic activities expressed as estradiol equivalent concentrations (EEQs) ranged from below detection (3 of 26 samples) to 40.7 ng/L, with a mean of 7.33 ng/L in all effluents. A good linear relationship was found between the EEQs measured by YES bioassay and the EEQs calculated from chemical concentrations. These detected estrogenic compounds, such as 4‐nonylphenol and estrone, were responsible for the estrogenic activities in the effluents. The genotoxic effects expressed as benzo[a]pyrene equivalent concentrations (BaP EQs) varied between below detection and 88.2 µg/L, with a mean of 8.76 µg/L in all effluents. The target polycyclic aromatic hydrocarbons were minor contributors to the genotoxicity in the effluents, and some nontarget compounds in the effluents were responsible for the measured genotoxicity. In terms of estrogenic activities and genotoxicity, discharge of these effluents could pose high risks to aquatic organisms in the receiving environments. Environ. Toxicol. Chem. 2012;31:1273–1282. © 2012 SETAC

show abstract

Evaluation of the new system (umu-test) for the detection of environmental mutagens and carcinogens

Cited by 593 publications

References 20 publications

Evaluation of wastewater reclamation technologies based on in vitro and in vivo bioassays

Evaluation of wastewater reclamation technologies based on in vitro and in vivo bioassays

Assessing of genotoxicity of 16 centralized source-waters in China by means of the SOS/umu assay and the micronucleus test: Initial identification of the potential genotoxicants by use of a GC/MS method and the QSAR Toolbox 3.0

Assessment of hormonal activities and genotoxicity of industrial effluents using in vitro bioassays combined with chemical analysis

Contact Info

Product

Resources

About