Evaluation of the Multiple Tissue Factors in the Cartilage of Primary and Secondary Rhinoplasty in Cleft Lip and Palate Patients

Buile, Dace; Pilmane, Māra; Akota, Ilze

doi:10.3390/pediatric14040050

Cited by 4 publications

(3 citation statements)

References 47 publications

(46 reference statements)

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“…The single-nucleotide polymorphism (SNP) within MMP9 , rs3918242, was considered a cleft palate risk factor, although GWAS did not support the role of rs3918242 in cleft palate (Letra et al , 2007; Kumari et al , 2019). However, increased MMP9-positive osteocytes in cleft palate patients’ alveolus/palate bone suggest a crucial downstream role of MMP9 in palatal development (Buile et al , 2022).…”

Section: Resultsmentioning

confidence: 99%

Filamin A mediates embryonical palatal fusion by linking mechanotransduction with β-Catenin/Smad2

Wang,

Hayano,

Weng

et al. 2024

Preprint

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To decipher potential mechanisms underlying cleft palate (CP), we used advanced bioinformatic integrated with literature mining and genome-wide association study (GWAS). Re-analysis of RNA-seq data (GSE45568, GSE185279) combined with literature mining highlighted the roles of Filamin A (Flna) and Epithelial-Mesenchymal Transition (EMT) in palatal development. Immunofluorescence of in vivo palatal shelves showed increased Flna in medial edge epithelial (MEE) cells and EMT cells located in an epithelial triangle. Inhibition of TGF-β or RhoA and mechanical stimuli impacted Flna expression in ex vivo cultured palatal shelves. Re-analysis of scRNA-seq data (GSE155928) highlighted a correlation between Flna and Ctnnb1 in EMT cells. Flna knockdown affected β-catenin/Smad2 expression in cultured palatal shelves and HaCaT cells. Epithelium-specific knockout of Flna delayed palatal fusion in female mice but not males. Mendelian randomization analysis suggested that parental habitual physical activity (HPA) was causally associated with lower risk of CP in their offspring. Together, these findings suggested that parental HPA could benefit their offspring's palatal development through Flna by linking mechanotransduction with the Wnt/TGF-β/Smad signaling pathways during palatal fusion.

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Section: Resultsmentioning

confidence: 99%

Filamin A mediates embryonical palatal fusion by linking mechanotransduction with β-Catenin/Smad2

Wang,

Hayano,

Weng

et al. 2024

Preprint

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“…Approximately 75% of all complete cleft lip and palate (CLP) patients are AC patients who require alveolar bone grafting (ABG) (Bangun et al, 2023;Destrez et al, 2023). AC, which is situated in the region bounded by the lateral incisors and canines, causes pathologic phonation, orofacial fistulas, fluid reflux, overcrowding, maxillary anterior-posterior and transverse defects, absence of bony support for maxillary anterior teeth, and disruption of the continuity of the maxillary arch (Gaillot et al, 2007;Guo et al, 2011;Tamasas and Cox, 2017;Ahmed et al, 2020;Buile et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Characterization and functional prediction of the dental plaque microbiome in patients with alveolar clefts

Zhang,

Zhi,

Shi

et al. 2024

Front. Cell. Infect. Microbiol.

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IntroductionAlveolar cleft (AC) is a common congenital defect in people with cleft lip and palate (CLP). Alveolar bone grafting (ABG) is typically performed during adolescence, resulting in the fissure remaining in the mouth for a longer length of time. Patients with AC have a greater rate of oral diseases such as dental caries than the normal population, and the precise characteristics of the bacterial alterations caused by AC are unknown.MethodsWe recruited a total of 87 subjects and collected dental plaque samples from AC adolescents (AAP), post-operative ABG adolescents (PAP), healthy control adolescents (CAP), AC young adults (AYP), post-operative ABG young adults (PYP), and healthy control young adults (CYP). The sequencing of 16S rRNA genes was performed.ResultsThe microbial composition of plaque from alveolar cleft patients differed significantly from age-matched healthy controls. Linear discriminant analysis effect size (LEfSe) analysis revealed that AAP was enriched for Neisseria, Haemophilus, Fusobacterium, Rhodococcus, Aggregatibacter, Gemella, and Porphyromonas, whereas AYP was enriched for Capnocytophaga, Rhodococcus, and Actinomyces-f0332. There were phenotypic differences in facultatively anaerobic, Gram-negative, Gram-positive, and oxidative stress tolerance between the AYP group with longer alveolar cleft and the healthy control group according to Bugbase phenotypic predictions. Alveolar bone grafting did not alter the functional phenotype of alveolar cleft patients but reduced the number of differential genera between alveolar cleft patients and healthy controls at both ages.ConclusionsOur study systematically characterized the supragingival plaque microbiota of alveolar cleft patients, post-alveolar bone grafting patients, and matched healthy controls in two ages to gain a better understanding of plaque ecology and microbiology associated with alveolar clefts.

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“…IL-10 has also been studied in oral health; it is expressed in the oral mucosa in relation to acute wounds [57]. Furthermore, IL-10 has been proven to be present in cleft patients' nasal septum cartilage and cleft lip tissues, where it takes part in the stimulation of cell proliferation and in providing a balance in the immune response [58,59].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Peptides and Interleukins in Cleft Soft Palate

2023

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Cleft palate is one of the most common and well-studied congenital anomalies; however, the role of protective tissue factors in its pathophysiology is still debated. The aim of our study was to evaluate interleukin and antimicrobial peptide appearance and distribution in cleft palate. Eight soft palate samples were obtained during veloplasty procedures. Immunohistochemical staining was applied to detect HBD-2-, HBD-3-, HBD-4-, LL-37-, IL-10-, and CD-163-positive cells via light microscopy. For statistical evaluation, the Mann–Whitney U test and Spearman’s rank correlation coefficient were used. A significant difference between study groups was observed for HBD-2 and IL-10 in epithelial and connective tissue as well as HBD-4 in connective tissue. The number of HBD-3-positive cells was moderate in the patients, and few were observed in the controls. The number of LL-37-positive cells varied from a moderate amount to a numerous amount in both study groups, whilst CD-163 marked a moderate number of positive cells in patients, and a few-to-moderate amount was observed in the controls. Numerous correlations between studied factors were revealed in cleft tissues. The increase in antimicrobial peptides HBD-2 and HBD-4 and anti-inflammatory cytokine IL-10 suggested a wide compensatory elevation of the local immune system against cleft-raised tissue changes. The correlations between the studied factors (HBD-2, HBD-3, HBD-4, LL-37, and IL-10) proved the synergistic involvement of common local defense factors in postnatal cleft palate morphopathogenesis.

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Evaluation of the Multiple Tissue Factors in the Cartilage of Primary and Secondary Rhinoplasty in Cleft Lip and Palate Patients

Cited by 4 publications

References 47 publications

Filamin A mediates embryonical palatal fusion by linking mechanotransduction with β-Catenin/Smad2

Filamin A mediates embryonical palatal fusion by linking mechanotransduction with β-Catenin/Smad2

Characterization and functional prediction of the dental plaque microbiome in patients with alveolar clefts

Antimicrobial Peptides and Interleukins in Cleft Soft Palate

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