Evaluation of the in vitro Production of Tumour Necrosis Factor by Monocytes in Dialysis Patients

Ryan, J. J.; Beynon, H. L. C.; Rees, Andrew J.; Cassidy, Michael J.

doi:10.1159/000170010

Cited by 17 publications

(14 citation statements)

References 18 publications

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“…In contrast to several other reports we did not find any significant difference in the amount of LPS-induced TN Fa activity between the HD groups and the controls [29][30][31]. There are several possible explanations for this discrep ancy.…”

Section: Discussioncontrasting

confidence: 99%

“…We confirmed our previous observations as well as those of others [17][18][19] that both pre-and post-HD PBMC produced significantly more TN Fa activity than the PBMC of normal controls. In addi tion we found that PBMC from HD patients produced significantly more TN Fa than PBMC from uremic patients not on dialysis who had TN Fa activity similar to that of controls.…”

Section: Discussionsupporting

confidence: 93%

“…Our finding that PBMC from HD patients on cellulose acetate membranes produced significantly higher levels of TN Fa than the PBMC of patients on CAPD confirms the results of two other studies comparing TN Fa production [18] and lL-6 levels [7]between HD and CAPD patients. The finding that CAPD PBMC under certain conditions pro duced significantly less TN Fa activity than normal PBMC may be due, at least in part, to the influence of the dialysate which has been shown to inhibit TN Fa production by normal PBMC [35].…”

Section: Discussionsupporting

confidence: 89%

“…It is more difficult to explain the difference in findings between our study and that of Ryan etal. [31] who used a similar biologi cal assay to detect TN Fa activity. However, they induced TN Fa activity in monocyte-enriched populations rather than whole PBMC and removed the TNFa-containing supernatant after a 24-hour rather than a 4-hour incubation period.…”

Section: Discussionmentioning

confidence: 99%

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Increased Production of Tumor Necrosis Factor Activity by Hemodialysis but Not Peritoneal Dialysis Patients

McKenna

Macdonald²,

Bernstein³

et al. 1994

Nephron

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The production of tumor necrosis factor alpha (TNFα) activity by peripheral blood mononuclear cells (PBMC) was determined in uremic patients on chronic hemodialysis (HD; n = 27), continuous ambulatory peritoneal dialysis (CAPD; n = 19), and in patients with chronic renal failure who were not yet on dialysis (CRF-ND; n = 18). In the HD group blood was taken immediately prior to and immediately following an HD session utilizing a cellulose acetate dialyzer. Post-HD PBMC spontaneously (i.e. in serum free media) produced significantly more TNFα activity than the PB MC of all other patient groups as well as those of the normal controls (n = 41) (p < 0.003). Post-HD PBMC produced significantly more TNFα activity than pre-HD PBMC both spontaneously and in the presence of nonuremic sera (p < 0.003). PBMC prior to HD also produced significantly more TNFα activity than CAPD PBMC and normal PBMC in the presence of autologous heat inactivated sera (p < 0.03). Under some culture conditions (i.e. in the presence of nonuremic sera) normal PBMC produced significantly (p < 0.003) more TNFα activity than CAPD PBMC. Finally, a positive correlation was found between PBMC TNFα activity and age for HD patients (r = 0.7, p < 0.004) but not for CAPD or CRF-ND patients. These findings suggest that PBMC of HD but not CAPD or CRF-ND patients are chronically stimulated to produce TNFα activity. Differences in TNFα activity production by PBMC of HD and CAPD patients may determine, at least in part, the types of short- and long-term complications seen in these patient populations.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Increased Production of Tumor Necrosis Factor Activity by Hemodialysis but Not Peritoneal Dialysis Patients

McKenna

Macdonald²,

Bernstein³

et al. 1994

Nephron

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“…Similar results were obtained for TNF [102,103] and IL-6 [104,105]. An IL-6 production (related to beta-2-microglobulin release) was observed, by our group, both before and after dialysis treatment with cellulosic membranes, suggesting a chronic or prolonged PBMC stimulation [106].…”

Section: Cytokine Production By Cultured Pbmcsupporting

confidence: 80%

Cytokine Production in Haemodialysis

Memoli

1999

Blood Purif

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Many aspects regarding morbidity and mortality of dialysis patients are related to the production of cytokines by peripheral blood mononuclear cells. Clinical alterations resulting from cytokine production and release may include dialysis amyloidosis, malnutrition and atherogenesis. Cytokine release may also play a relevant role in immunodeficiency of dialysis patients by inducing alterations in immune and host-defense system. Interleukin-1, interleukin-6 and tumor necrosis factor are three pro-inflammatory cytokines, mainly produced by monocytes, and involved in pathogenetic aspects of hemodialysis-related diseases. In this review we analyse the mechanisms underlying monocyte activation and describe the different modalities for studying cytokine production and release. Clinical implications of cytokine production are also discussed.

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