Evaluation of the effects of nano-curcumin on the expression of genes involved in biofilm formation in Staphylococcus epidermidis

Shivaee, Ali; Meskini, Maryam; Roodaki, Rojin Rahimi Nejad; Kalani, Behroz Sadeghi; Mirshekar, Maryam; Razavi, Shabnam

doi:10.1016/j.genrep.2021.101026

Cited by 6 publications

(2 citation statements)

References 29 publications

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“…hospital acquired infections. It is reported that E. coli causes 90% of community and 50% of nosocomial UTIs [31,32]. The current study provided useful information on the antimicrobial resistance model, biofilm production, virulence factor, and quinolone-nonsusceptible E. coli (isolated from different samples) to aid effective empirical treatment choices.…”

Section: Discussionmentioning

confidence: 90%

Determination of virulence and quinolone resistance genes and biofilm production among uropathogenic Escherichia coli strains isolated from clinical specimens in Iran

Tabatabaie

Badamchi

Ohadi

et al. 2021

Reviews in Medical Microbiology

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Background: Uropathogenic Escherichia coli (UPEC) is the main cause of nosocomial urinary tract infections. The high rates of antimicrobial resistance among UPEC have markedly reduced the remedial options and raised the therapeutic costs and fatality rates. This study analyzed the ability of extended spectrum beta-lactamase (ESBL) production and the presence of plasmid-mediated quinolone resistance genes among UPEC isolates. It also assessed the correlation between plasmid-mediated quinolone resistance genes, biofilm production, and virulence factors among these isolates. Materials and methods:A number of 300 UPEC isolates were recovered from patients with typical clinical signs and symptoms of cystitis, and pyelonephritis, as well as those with asymptomatic bacteriuria. Hemolysis assay, antimicrobial susceptibility testing, and biofilm production were investigated among these isolates. These samples were analyzed by multiplex PCR on genes encoding the pursuits among the virulence factors: type 1 fimbrial adhesin (FimH), pyelonephritis-associated pili (PaP), S fimbriae (Sfa), afimbrial adhesin I (AfaI), hemolysin (hly), aerobactin (Aer), flagelares de la fase 1 (FliC), cytotoxic necrotizing factor 1 (Cnf1), IroN-uptake systems of E. coli D (IucD), cytolethal distending toxin (CDT), and uropathogenicspecific protein (Usp) operon. Results:The current study showed that the presence of virulence genes render the UPEC isolates higher resistance to antibiotics and a higher ability of biofilm production. Conclusion:The fact that the infection can be highly affected by virulence factors of E. coli, let us to conclude that in the case of signs and symptoms of urinary tract infection, it is better to avoid wide use of antibiotics.

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Section: Discussionmentioning

confidence: 90%

Determination of virulence and quinolone resistance genes and biofilm production among uropathogenic Escherichia coli strains isolated from clinical specimens in Iran

Tabatabaie

Badamchi

Ohadi

et al. 2021

Reviews in Medical Microbiology

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“…Biofilm formation in the K. pneumoniae isolates was determined [19] using microtitre plate assay. Briefly, the overnight grown isolates were cultured into the microtiter plates for 48 h. Thereafter, the wells were washed, and biofilms were stained with crystal violet.…”

Section: Biofilm Assaymentioning

confidence: 99%

Evaluation of biosynthesized silver nanoparticles effects on expression levels of virulence and biofilm‐related genes of multidrug‐resistant Klebsiella pneumoniae isolates

et al. 2023

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The emergence of multidrug‐resistant (MDR) strains of Klebsiella pneumoniae is associated with high morbidity and mortality due to limited treatment options. This study attempts to biologically synthesize silver nanoparticles (AgNPs) and investigate their effect on expression levels of virulence and biofilm‐related genes in clinically isolated K. pneumoniae. In this study, biofilm formation ability, antibiotic resistance pattern, extended‐spectrum β‐lactamases (ESBLs), and carbapenemases production were investigated for 200 clinical isolates of K. pneumoniae using phenotypic methods. Polymerase chain reaction (PCR) was used to detect virulence and biofilm‐related genes, ESBL‐encoding genes, and carbapenem resistance genes. AgNPs were synthesized using the bio‐reduction method. The antibacterial effects of AgNPs were investigated by microdilution broth. In addition, the cytotoxic effect of AgNPs on L929 fibroblast cell lines was determined. The effects of AgNPs on K. pneumoniae virulence and biofilm‐related genes (fimH, rmpA, and mrkA) were determined using quantitative real‐time PCR. Thirty percent of the isolates produced a strong biofilm. The highest and lowest levels of resistance were observed against amoxicillin/clavulanic acid (95.4%) and tigecycline (96%), respectively. About 31% of isolates were considered positive for carbapenemases, and 75% of the isolates produced an ESBLs enzyme. Different frequencies of mentioned genes were observed. The synthesized AgNPs had a spherical morphology and varied in size. AgNPs inhibited the growth of MDR K. pneumoniae at 128 µg/ml. In addition, AgNPs downregulated the expression of fimH, rmpA, and mrkA genes by 10, 7, and 14‐fold, respectively (p < 0.05), also exerted no cytotoxic effect on L929 fibroblast cell lines. It was revealed that AgNPs lead to a decrease in expression levels of virulence and biofilm‐related genes; therefore, it was concluded that AgNPs had an excellent antibacterial effect on MDR K. pneumoniae.

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Nanoform of curcumin: expansion in therapeutic applications

Mohanty,

Yadav

2024

ADV TRADIT MED (ADTM)

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Evaluation of the effects of nano-curcumin on the expression of genes involved in biofilm formation in Staphylococcus epidermidis

Cited by 6 publications

References 29 publications

Determination of virulence and quinolone resistance genes and biofilm production among uropathogenic Escherichia coli strains isolated from clinical specimens in Iran

Determination of virulence and quinolone resistance genes and biofilm production among uropathogenic Escherichia coli strains isolated from clinical specimens in Iran

Evaluation of biosynthesized silver nanoparticles effects on expression levels of virulence and biofilm‐related genes of multidrug‐resistant Klebsiella pneumoniae isolates

Nanoform of curcumin: expansion in therapeutic applications

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