2010
DOI: 10.1590/s0001-37652010000400011
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Evaluation of the botanical origin of commercial dry bee pollen load batches using pollen analysis: a proposal for technical standardization

Abstract: High quality of bee pollen for commercial purpose is required. In order to attend the consumer with the best identification of the botanical and floral origin of the product, 25 bee pollen batches were investigated using two techniques of pollen grain preparation. The first started to identify pollen loads of different colors in two grams of each well mixed batch, and the second to identify pollen grains in a pool made of all the pollen loads comprised in two grams. The best result was obtained by this last te… Show more

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Cited by 68 publications
(55 citation statements)
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“…The procedure followed the methodology proposed by Barth et al (2010) using two grams of each sample of dried bee pollen homogenate stirring in 70% ethanol. Centrifuge tubes were filled to 13 mL, and the material remained at rest for 30 min or overnight and was then submitted to sonication for five minutes to dissociate the pollen grains.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The procedure followed the methodology proposed by Barth et al (2010) using two grams of each sample of dried bee pollen homogenate stirring in 70% ethanol. Centrifuge tubes were filled to 13 mL, and the material remained at rest for 30 min or overnight and was then submitted to sonication for five minutes to dissociate the pollen grains.…”
Section: Methodsmentioning
confidence: 99%
“…Regional data were presented by Barth & Luz (1998) with regard to pollen collection inside mangrove areas, Barth (2004) in a review, Modro et al (2007), Luz et al (2007) in a forested area of Rio de Janeiro State, Melo et al (2009) in São Paulo State, Barth et al (2009), Luz et al (2010 in Minas Gerais State, Barth et al (2010) with regard to the technical processing of pollen loads, Luz et al (2011) in Espírito Santo State, Boff et al (2011) The present study aimed to investigate the botanical and geographical origins of bee pollen collected in four Brazilian macro-regions. This study aimed to provide also results that will contribute to improve the certification of this bee product and the preservation of the native vegetation.…”
mentioning
confidence: 99%
“…[13]. Dry pollen loads of 2 g of a sample (this is a pool of circa 300 pollen loads) were mixed with 70% ethanol to complete 13 mL, and sonicated for 5 min.…”
Section: Preparation Of Pollen For Microscopymentioning
confidence: 99%
“…The resultant mixtures were centrifuged at 5,000 rpm for 10 min, and the supernatants were used for HPLC/DAD analysis as previously described in Refs. [11,13] and for oxygen radical absorbance capacity (ORAC) assays as described in Ref. [14].…”
Section: Sample Preparationmentioning
confidence: 99%
“…Rico em proteínas, apresenta importância vital no desenvolvimento das larvas (9,10) ; além disso, o pólen também constitui a principal fonte de graxas, vitaminas e sais minerais para as abelhas e a sua ausência pode levar a colmeia à extinção (9,10) . Devido à demanda comercial, a produção de pólen apícola aumentou nos últimos anos e a abelha Apis mellífera destaca-se como a melhor fornecedora deste produto (11) . A quantidade de pólen colhido por uma colônia de A. mellifera depende da quantidade de operárias forrageiras e da cria (ovos -larvas) presente nesta colônia (12) .…”
Section: Introductionunclassified