2019
DOI: 10.1111/tan.13708
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Abstract: HLA genotyping by next‐generation sequencing is now widely performed. We aimed at evaluating the performance of the One Lambda AllType kit using Thermo Fisher Scientific reagents on the Ion S5 XL platform. Reads were analyzed using the TypeStream Visual software. We performed 15 runs between April and September 2018 to type DNA at the HLA‐A/B/C/DRB1/3/4/5/DQA1/DQB1/DPA1/DPB1 loci from 340 samples and 15 positive controls. We observed only seven (0.1%) critical mistakes among the 6009 alleles typed, correspondi… Show more

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Cited by 141 publications
(140 citation statements)
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“…Now common HLA genotyping methods were includedpolymerase chain reaction sequencebased typing (PCR-SBT), PCRsequence-speci c primer, PCR sequence-speci c oligonucleotide,next-generation sequencing (NGS) and so on. NGS method for HLAgenotyping has characteristic of economical, rapid andhigh-throughput, which can be improved allelic resolution and fewertyping ambiguities [6][7][8][9][10][11][12]. As the rapidly evolution of precise HLAgenotyping technology, 26,214 HLA alleles have been identi edaccording to the IPD-IMGT/HLA database (IPD-IMGT/HLA release3.39.0; January 2020).…”
Section: Introductionmentioning
confidence: 99%
“…Now common HLA genotyping methods were includedpolymerase chain reaction sequencebased typing (PCR-SBT), PCRsequence-speci c primer, PCR sequence-speci c oligonucleotide,next-generation sequencing (NGS) and so on. NGS method for HLAgenotyping has characteristic of economical, rapid andhigh-throughput, which can be improved allelic resolution and fewertyping ambiguities [6][7][8][9][10][11][12]. As the rapidly evolution of precise HLAgenotyping technology, 26,214 HLA alleles have been identi edaccording to the IPD-IMGT/HLA database (IPD-IMGT/HLA release3.39.0; January 2020).…”
Section: Introductionmentioning
confidence: 99%
“…While sequencing of the whole genome [4], whole exome [5,6], transcriptome [7,8], or captured HLA genes can lead to successful HLA typing, most commercially available NGS assays amplify target HLA genes by polymerase chain reaction (PCR) followed by library preparation and sequencing [9][10][11][12]. One common approach is the "long-amplicon, shortread" approach.…”
Section: Introductionmentioning
confidence: 99%
“…We report here a novel HLA‐DRB3*02 allele, DRB3*02:142 that carries one nucleotide substitution in exon 3 when compared with the DRB3*02:02:01:02 allele, identified in a volunteer bone marrow donor. The HLA typing was performed using next‐generation sequencing (AllType NGS, One Lambda, Canoga Park, California) on the Ion S5 system platform (ThermoFisher Scientific, Waltham, Massachusetts), from exons 2 to 5. The reads were analyzed using the TypeStream Visual Software version 1.3.0.27232 (One Lambda).…”
mentioning
confidence: 99%