Evaluation of temperature induction in focal ischemic thermocoagulation model

Silva, Helio da; Nucci, Mariana Penteado; Mamani, Javier Bustamante; Mendez-Otero, Rosália; Nucci, Leopoldo Penteado; Tannús, Alberto; Gamarra, Lionel Fernel

doi:10.1371/journal.pone.0200135

Cited by 9 publications

(9 citation statements)

References 54 publications

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“…Induction of a focal ischemic lesion via thermocoagulation (stroke): A focal brain ischemic lesion was induced via thermocoagulation in the pial blood vessels of the motor and somatosensory cortex as previously described[21]. Briefly, animals were anesthetized with ketamine hydrochloride (100 mg/kg, i.p.)…”

Section: Methodsmentioning

confidence: 99%

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Triple-modal imaging of stem-cells labeled with multimodal nanoparticles, applied in a stroke model

Silva

Mamani

Nucci

et al. 2019

WJSC

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BACKGROUND Mesenchymal stem cells (MSCs) have been widely tested for their therapeutic efficacy in the ischemic brain and have been shown to provide several benefits. A major obstacle to the clinical translation of these therapies has been the inability to noninvasively monitor the best route, cell doses, and collateral effects while ensuring the survival and effective biological functioning of the transplanted stem cells. Technological advances in multimodal imaging have allowed in vivo monitoring of the biodistribution and viability of transplanted stem cells due to a combination of imaging technologies associated with multimodal nanoparticles (MNPs) using new labels and covers to achieve low toxicity and longtime residence in cells. AIM To evaluate the sensitivity of triple-modal imaging of stem cells labeled with MNPs and applied in a stroke model. METHODS After the isolation and immunophenotypic characterization of human bone marrow MSCs (hBM-MSCs), our team carried out lentiviral transduction of these cells for the evaluation of bioluminescent images (BLIs) in vitro and in vivo . In addition, MNPs that were previously characterized (regarding hydrodynamic size, zeta potential, and optical properties), and were used to label these cells, analyze cell viability via the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay and BLI analysis, and quantify the internalization process and iron load in different concentrations of MNPs via magnetic resonance imaging (MRI), near-infrared fluorescence (NIRF), and inductively coupled plasma-mass spectrometry (ICP-MS). In in vivo analyses, the same labeled cells were implanted in a sham group and a stroke group at different times and under different MNP concentrations (after 4 h or 6 d of cell implantation) to evaluate the sensitivity of triple-modal images. RESULTS hBM-MSC collection and isolation after immunophenotypic characterization were demonstrated to be adequate in hBM samples. After transduction of these cells with luciferase (hBM-MSC Luc ), we detected a maximum BLI intensity of 2.0 x 10 8 photons/s in samples of 10 6 hBM-MSCs. Analysis of the physicochemical characteristics of the MNPs showed an average hydrodynamic diameter of 38.2 ± 0.5 nm, zeta potential of 29.2 ± 1.9 mV and adequate colloidal stability without agglomeration over 18 h. The signal of iron load internalization in hBM-MSC Luc showed a close relationship with the corresponding MNP-labeling concentrations based on MRI, ICP-MS and NIRF. Under the highest MNP concentration, cellular viability showed a reduction of less than 10% compared to the control. Correlation analysis of the MNP load internalized into hBM-MSC ...

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Section: Methodsmentioning

confidence: 99%

“…The ischemic lesion was confirmed through local blood perfusion analysis using a PeriCam Perfusion Speckle Imager (PSI) system (Perimed, Stockholm, Sweden) and TTC staining after 2 h of lesion induction, as described in a previous study[21]. The color changes of the targeted region, from light red to dark red, were also noted[22].…”

Section: Methodsmentioning

confidence: 99%

Triple-modal imaging of stem-cells labeled with multimodal nanoparticles, applied in a stroke model

Silva

Mamani

Nucci

et al. 2019

WJSC

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“…to bregma). Transdural thermocoagulation of blood in the superficial vessels was performed by approaching a hot probe adjusted to 300 °C temperature 39 . Vessel lesions were evaluated macroscopically through the colour changes after 5 min.…”

Section: Methodsmentioning

confidence: 99%

Comparative effects of dexmedetomidine and propofol on brain and lung damage in experimental acute ischemic stroke

Sousa

Fernandes

Cruz

et al. 2021

Sci Rep

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Acute ischemic stroke is associated with pulmonary complications, and often dexmedetomidine and propofol are used to decrease cerebral metabolic rate. However, it is unknown the immunomodulatory actions of dexmedetomidine and propofol on brain and lungs during acute ischemic stroke. The effects of dexmedetomidine and propofol were compared on perilesional brain tissue and lung damage after acute ischemic stroke in rats. Further, the mean amount of both sedatives was directly evaluated on alveolar macrophages and lung endothelial cells primarily extracted 24-h after acute ischemic stroke. In twenty-five Wistar rats, ischemic stroke was induced and after 24-h treated with sodium thiopental (STROKE), dexmedetomidine and propofol. Dexmedetomidine, compared to STROKE, reduced diffuse alveolar damage score [median(interquartile range); 12(7.8–15.3) vs. 19.5(18–24), p = 0.007)], bronchoconstriction index [2.28(2.08–2.36) vs. 2.64(2.53–2.77), p = 0.006], and TNF-α expression (p = 0.0003), while propofol increased VCAM-1 expression compared to STROKE (p = 0.0004). In perilesional brain tissue, dexmedetomidine, compared to STROKE, decreased TNF-α (p = 0.010), while propofol increased VCAM-1 compared to STROKE (p = 0.024). In alveolar macrophages and endothelial cells, dexmedetomidine decreased IL-6 and IL-1β compared to STROKE (p = 0.002, and p = 0.040, respectively), and reduced IL-1β compared to propofol (p = 0.014). Dexmedetomidine, but not propofol, induced brain and lung protection in experimental acute ischemic stroke.

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“…Animals were randomly allocated to three treatment groups: sham, ischemic (ISC), and ischemic treated with EVs (ISC + EV). Our stroke model of focal cerebral permanent ischemia represents a protocol to investigate brain lesions with good reproducibility and low mortality [ 17 , 18 , 19 , 20 , 21 , 50 , 68 , 69 ].…”

Section: Methodsmentioning

confidence: 99%

Functional Recovery Caused by Human Adipose Tissue Mesenchymal Stem Cell-Derived Extracellular Vesicles Administered 24 h after Stroke in Rats

Rohden

Teixeira

Bernardi

et al. 2021

IJMS

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Ischemic stroke is a major cause of death and disability, intensely demanding innovative and accessible therapeutic strategies. Approaches presenting a prolonged period for therapeutic intervention and new treatment administration routes are promising tools for stroke treatment. Here, we evaluated the potential neuroprotective properties of nasally administered human adipose tissue mesenchymal stem cell (hAT-MSC)-derived extracellular vesicles (EVs) obtained from healthy individuals who underwent liposuction. After a single intranasal EV (200 µg/kg) administered 24 h after a focal permanent ischemic stroke in rats, a higher number of EVs, improvement of the blood–brain barrier, and re-stabilization of vascularization were observed in the recoverable peri-infarct zone, as well as a significant decrease in infarct volume. In addition, EV treatment recovered long-term motor (front paws symmetry) and behavioral impairment (short- and long-term memory and anxiety-like behavior) induced by ischemic stroke. In line with these findings, our work highlights hAT-MSC-derived EVs as a promising therapeutic strategy for stroke.

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Evaluation of temperature induction in focal ischemic thermocoagulation model

Cited by 9 publications

References 54 publications

Triple-modal imaging of stem-cells labeled with multimodal nanoparticles, applied in a stroke model

Triple-modal imaging of stem-cells labeled with multimodal nanoparticles, applied in a stroke model

Comparative effects of dexmedetomidine and propofol on brain and lung damage in experimental acute ischemic stroke

Functional Recovery Caused by Human Adipose Tissue Mesenchymal Stem Cell-Derived Extracellular Vesicles Administered 24 h after Stroke in Rats

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