Evaluation of synthesized platinum nanoparticles on the MCF-7 and HepG-2 cancer cell lines

Abstract

Platinum nanoparticles (PNPs) were synthesized by chemical reduction of potassium hexachloroplatinate (IV) with trisodium citrate under vigorous stirring and addition of sodium dodecyl sulfate as stabilizer reagent. Reducing agent was chosen depending on the oxidation reactions and potential values of the chemical materials used in the experiment. The aim of this study is to investigate the effects of PNPs on the different cancer cell lines and cytotoxicity study of this nanomaterial. The morphology of PNPs was investigated by scanning electron microscope (XL30, Philips Electronics, Amsterdam, The Netherlands) with the ability to perform elemental analysis by EDX. Malvern Zetasizer 3000 HSA (Malvern Instruments, Worcestershire, UK) was used to determine the distribution of particle size and zeta potential of PNPs. The cytotoxicity property of the nanoparticles was evaluated by MTT assay on MCF-7 and HepG-2 cell lines, and the cytotoxic concentration 50% values were determined for 24 h.

Synthesis and Cytotoxicity of Dendritic Platinum Nanoparticles with HEK‐293 Cells

“…[28][29][30][31] It is of high interest to expand the applicability of Pt nanoparticles over multiple fields, therefore, through our study,w eaimed to achievea ne nhanced performance for biomedicala pplicationsu sing size-controlledD PNs, prepared with an amphiphilic molecular surfactant and as onochemical method.N anoporous architecturesw ith high surface areas and larger pore volumes can accommodate av ariety of drugs, making these useful for drugd elivery.Nanoparticles for biomedical applications have shown enormous potentiali nr ecent years as therapeutic mediators for many diseases, including cancer. [32][33][34] One of the major limitations, however,i nu sing metallic nanoparticles, is their nonspecific untargeted toxicity. To apply our DPNs in biomedical fields,i ti sn ecessary to understandv ariousp rocesses induced by these nanoparticles in human tissues and genetic material to furtheri mprovet heir sensitizingp roperties.H ence,f or any new materialb eing developed for therapeutic purposes, the determination of its toxicity to human tissues is highly important.…”

“…Nanoparticles for biomedical applications have shown enormous potentiali nr ecent years as therapeutic mediators for many diseases, including cancer. [32][33][34] One of the major limitations, however,i nu sing metallic nanoparticles, is their nonspecific untargeted toxicity. To apply our DPNs in biomedical fields,i ti sn ecessary to understandv ariousp rocesses induced by these nanoparticles in human tissues and genetic material to furtheri mprovet heir sensitizingp roperties.H ence,f or any new materialb eing developed for therapeutic purposes, the determination of its toxicity to human tissues is highly important.…”

Manufacturing nanomaterials: from research to industry

“…Chemical reduction techniques have been extensively investigated in the preparation of platinum nanoparticles because these methods can be implemented under simple and mild conditions, and can be used to prepare nanoparticles on a large scale. Platinum nanoparticles can be synthesized by chemical reduction of potassium hexachloroplatinate with trisodium citrate under vigorous stirring and addition of sodium dodecyl sulfate as stabilizer reagent [123]. Except for platinum nanoparticles, 3D platinum nanoflowers can be synthesized by a facile and scalable approach.…”

Heterocoagulation of polysaccharide-coated platinum nanoparticles with ovarian-cancer cells

“…The authors of [35] mistakenly related the stability of the dispersion of Pt SDS NPs in the DMEM nutrient medium (the medium additionally contained fetal bovine serum (FBS) (10%), glutamine, and antibiot ics) to the high NP ζ potential resulting from the adsorption of the anionic surfactant. The matter is that the ζ potential may be correctly measured (a Zetasizer instrument was used) only in a diluted electrolyte; however, in the nutrient medium with a high electro lyte concentration, the electrical double layer was compressed, thereby leading to a drastic fall in the ζ potential.…”

“…The influence of platinum NPs (Pt SDS ) synthesized by the citrate method and stabilized with an anionic surfactant, sodium dodecyl sulfate (SDS), on liver and mammary gland cancer cells of the HEOG 2 and MCF 7 lines, respectively, was investigated in [35]. The MTT test was used to establish the critical con centrations (IC50) of Pt SDS NPs at which 50% of cells died over a preset contact time (in this case, 24 h).…”