Evaluation of stationary phases packed with superficially porous particles for the analysis of pharmaceutical compounds using supercritical fluid chromatography

Core–Shell, Ultrasmall Particles, Monoliths, and Other Support Materials in High-Performance Liquid Chromatography

“…While the original particle size of the new generation shell column was of the order of 2.5 m, columns packed with sub 2m shell particles are now widely commercially available and can give further improvements in performance, if appropriate instrumentation is available 31,32 . This improvement can be seen for the kinetic plots in Fig.…”

“…While the original particle size of the new generation shell column was of the order of 2.5 μm, columns packed with sub 2 μm shell particles are now widely commercially available and can give further improvements in performance, if appropriate instrumentation is available. , This improvement can be seen for the kinetic plots in Figure b where separations requiring around 20 000 plates can be achieved in around 1 min on a 1.6 μm shell column but require significantly longer on the totally porous column of about the same particle size (1.8 μm).…”

Design and pharmaceutical applications of porous particles

“…But there are still supercially porous silica (SPSi) which consists of a non-porous silica core surrounded by a thin shell of porous silica (typically of 1-2 mm thickness), with overall particle size being $30 mm. 61,62 Such particles are usually used as a stationary phase for chromatography to separate biomolecules. Those with pore diameter of $100Å can be used for separating small molecules and those with 160-200Å pore diameter are available for separating peptides and small proteins.…”

Parameter Optimization for Feature and Hit Generation in a General Unknown Screening Method—Proof of Concept Study Using a Design of Experiment Approach for a High Resolution Mass Spectrometry Procedure after Data Independent Acquisition

“…A more progressive strategy is using a design of experiment (DoE) approach where multiple parameters can be evaluated simultaneously. DoE approaches are already widely applied in bioanalytical science, emergency toxicology, environmental analytics, food analytics, , metabolomics, − pharmaceutical analytics, − and water analytics mostly for optimization of the chromatographic separation.…”