2008
DOI: 10.1007/s12355-008-0060-7
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Abstract: Smut disease of sugarcane, caused by the fungus Ustilago scitaminea Sydow, can cause considerable yield losses and reductions in cane quality. To investigate the reactions of the seedlings to smut, three different inoculation methods were employed. Data on number of smut whips per stool, disease incidents per population and number of tillers per plot were recorded and analysed. Results showed that screening for smut resistance at the first stage of selection to assess seedling reaction to smut is possible. The… Show more

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Cited by 14 publications
(3 citation statements)
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“…Single budded sets of 7 month-old plants were surface disinfected, heat treated (52°C for 30 min in water bath, 1 kg of buds/6L of water) and incubated for 16 h at 28°C. Sets were then placed on trays with buds facing upwards, and inoculated using the wound-paste method [ 43 ]. Pots were kept in the greenhouse arranged into a completely randomized experimental design.…”
Section: Methodsmentioning
confidence: 99%
“…Single budded sets of 7 month-old plants were surface disinfected, heat treated (52°C for 30 min in water bath, 1 kg of buds/6L of water) and incubated for 16 h at 28°C. Sets were then placed on trays with buds facing upwards, and inoculated using the wound-paste method [ 43 ]. Pots were kept in the greenhouse arranged into a completely randomized experimental design.…”
Section: Methodsmentioning
confidence: 99%
“…Pre-germinated buds of the sugarcane cv. Co 97009 were inoculated with teliospores (4 x 10 6 spores/mL) of S. scitamineum isolates Ss97009 and SsV89101 by bud pasting method ( Co et al., 2008 ), planted, and maintained under glasshouse conditions at 28°C - 30°C with 60-80% humidity. At least twenty plants were inoculated per treatment and an equal number of uninoculated plants served as control.…”
Section: Methodsmentioning
confidence: 99%
“…Several studies have been conducted for comparing different inoculation methods against various pathogens for screening different varieties. Co et al [15] evaluated three different smut inoculation techniques: soaking, wounding along with paste and pastes without wounding in sugarcane seedlings and found wounding along with paste method was the best method. Bayern and Sharma [16] compared five stem inoculation methods with respect to phytoalexin accumulation and Fusarium wilt development in carnation and found injection method to be the more effective method.…”
Section: Pathogenicity Of C Gloeosporioidesmentioning
confidence: 99%