Evaluation of seven PCR-based assays for the analysis of microchimerism

Sahota, Amrik; Yang, Min; McDaniel, Huey G.; Sidner, Richard A.; Book, Benita K.; Barr, Robert C.; Brahmi, Zacharie; Jindal, Rahul M.

doi:10.1016/s0009-9120(98)00059-9

Cited by 18 publications

(11 citation statements)

References 22 publications

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“…Transplanted solid organs contain mature lymphocytes capable of inducing GvHD as well as small numbers of HSCs. It has been known for some time that many recipients of solid organ transplants become microchimeric post-transplant [11].…”

Section: Microchimerism and Solid Organ Transplanationmentioning

confidence: 99%

Concise Review: Expanding Roles for Hematopoietic Cellular Therapy and the Blood Transfusion Services

Hodby

Pamphilon

2011

Stem Cells

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Hematopoietic stem cells (HSCs) have remained at the forefront of stem cell research for the past 50 years, since the therapeutic potential of bone marrow transplantation was realized. Uniquely, among stem and progenitor cells, research progress has been made in parallel between the laboratory benchtop and hospital bedside during this period. Integral to this work has been the role of the transfusion medicine services in the collection, storage, and processing of HSCs. The next decade promises to bring further developments: with new fields of cellular therapies, stem cell vaccination, and stem cell drug testing opening up. This article summarizes exciting areas of research concerning the behavior and potential clinical applications of HSCs. For the purposes of clarity, we describe in turn the trafficking and transfer of HSCs; ex vivo expansion of HSC units from different sources; and finally, applications of specifically selected subsets of hematopoietic cells and their progeny.

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Section: Microchimerism and Solid Organ Transplanationmentioning

confidence: 99%

Concise Review: Expanding Roles for Hematopoietic Cellular Therapy and the Blood Transfusion Services

Hodby

Pamphilon

2011

Stem Cells

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“…It must also be noted that the majority of such studies have been performed on female recipients of male, whole unfiltered blood. This allows for the design of highly specific PCR strategies to be directed at regions of the Y chromosome, alleviating problems caused by interference of recipient DNA [20].…”

Section: Peri-mortem Blood Transfusion and Microchimerismmentioning

confidence: 99%

Can post-mortem blood be used for DNA profiling after peri-mortem blood transfusion?

Graham

Tsokos²,

Rutty

2005

Int J Legal Med

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The question of whether blood transfusions can affect DNA profiling is still a contentious issue throughout the forensic community. It is hypothesised that donor leucocytes present in the administered blood will be detected upon examination of recipient blood. In order to resolve this issue, a selection of theoretical experiments were carried out to determine how much donor DNA must be present for its detection in blood components. Five casework examples of material collected from individuals after massive transfusion, including a case of whole organ transplantation, were also investigated. The results indicated that filtration processes used during blood production do not allow the passage of enough donor leucocytes for detection using current forensic profiling techniques. No evidence of secondary profile alleles were found in any case, indicating that peri-mortem blood transfusion does not affect DNA profiling.

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“…35 Regardless of the assay used, the detection of rare populations of cells in blood remains challenging. Assays range in sensitivity from 0.1% male DNA in a female DNA background using single primer pairs for the Y chromosome to 0.0001% with nested primers.…”

Section: Survival Of Transfused Donor White Blood Cells 275mentioning

confidence: 99%

“…Assays range in sensitivity from 0.1% male DNA in a female DNA background using single primer pairs for the Y chromosome to 0.0001% with nested primers. 35 Different primers may lead to different amplification efficiencies, and inadvertent mispriming of associated material, such as pseudogenes in the recipient, has led to false-positive results because of misinterpretation of the bands as of donor origin. 36 We controlled for this possibility by running coded preand posttransfusion samples from each case together.…”

Section: Survival Of Transfused Donor White Blood Cells 275mentioning

confidence: 99%

Survival of transfused donor white blood cells in HIV-infected recipients

Kruskall

Lee

Assmann

et al. 2001

Blood

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The appearance and expansion of donor white blood cells in a recipient after transfusion has many potential biologic ramifications. Although patients with HIV infection are ostensibly at high risk for microchimerism, transfusion-associated graft-versus-host disease (TA-GVHD) is rare. The purpose of this study was to search for sustained microchimerism in such patients. Blood samples were collected from 93 HIV-infected women (a subset from the Viral Activation Transfusion Study, an NHLBI multicenter randomized trial comparing leukoreduced versus unmodified red blood cell [RBC] transfusions) before and after transfusions from male donors. Donor lymphocytes were detected in posttransfusion specimens using a quantitative Y-chromosome-specific polymerase chain reaction (PCR) assay, and donor-specific human leukocyte antigen (HLA) alleles were identified with allele-specific PCR primers and probes. Five of 47 subjects randomized to receive nonleukoreduced RBCs had detectable male lymphocytes 1 to 2 weeks after transfusion, but no subject had detectable male cells more than 4 weeks after a transfusion. In 4 subjects studied, donorspecific HLA haplotypes were detected in posttransfusion specimens, consistent with one or more donors' cells. None of 46 subjects randomized to receive leukoreduced RBCs had detectable male lymphocytes in the month after transfusion. Development of sustained microchimerism after transfusion in HIV-infected patients is rare; HIV-infected patients do not appear to be at risk for TA-GVHD. (Blood. 2001;98:272-279)

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Evaluation of seven PCR-based assays for the analysis of microchimerism

Cited by 18 publications

References 22 publications

Concise Review: Expanding Roles for Hematopoietic Cellular Therapy and the Blood Transfusion Services

Concise Review: Expanding Roles for Hematopoietic Cellular Therapy and the Blood Transfusion Services

Can post-mortem blood be used for DNA profiling after peri-mortem blood transfusion?

Survival of transfused donor white blood cells in HIV-infected recipients

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