Evaluation of serological tests for the diagnosis of Neospora caninum infection in dogs: Optimization of cut off titers and inhibition studies of cross-reactivity with Toxoplasma gondii

Silva, Deise A. O.; Lobato, Janaina; Mineo, Tiago W. P.; Mineo, José Roberto

doi:10.1016/j.vetpar.2006.08.028

Cited by 76 publications

(71 citation statements)

References 35 publications

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“…This technique would be especially useful in dogs, for which current serological techniques lack the sensitivity and specificity of those used in bovines (Silva et al 2007, Ghalmi et al 2009). …”

Section: Discussionmentioning

confidence: 99%

Characterisation of NcGRA7 and NcSAG4 proteins: Immunolocalisation and their role in the host cell invasion by Neospora caninum tachyzoites

Aguado‐Martínez

Álvarez‐García

Schares

et al. 2010

Acta Parasitologica

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Neospora caninum negatively impacts bovine reproductive performance around the world. Addressing this problem requires a greater understanding of the parasite's molecular biology. In this study, monoclonal antibodies against recombinant proteins were successfully developed and employed to characterise two different proteins of N. caninum: the acute phase-associated NcGRA7 and the chronic phase-associated NcSAG4. Immunofluorescence with the anti-rNcGRA7 monoclonal antibody suggested that NcGRA7 trafficks from tachyzoite dense granules to the matrix of the parasitophorous vacuole and parasite's surroundings. Furthermore, NcGRA7 is also expressed in the bradyzoite stage and localised on the matrix of bradyzoite-positive vacuoles. NcGRA7 appears to be partially involved in the tachyzoite-invasion mechanisms, as an anti-rNcGRA7 monoclonal antibody partially inhibited in vitro tachyzoite-invasion. A monoclonal antibody specific for NcSAG4 confirmed this protein's bradyzoitespecific expression both by western blot and immunofluorescence. However, some bradyzoite-positive vacuoles only weakly expressed NcSAG4, if it was expressed at all. The specificity of the anti-rNcSAG4 monoclonal antibody was confirmed by the recognition of the NcSAG4 in the membrane surface of Nc-1SAG4 c transgenic tachyzoites, which constitutively expresses NcSAG4. Blocking NcSAG4 of Nc-1SAG4 c tachyzoites with the monoclonal antibody did not affect host cell invasion. However, its implication on the host cell adhesion or host immune evasion should not be discarded.

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Section: Discussionmentioning

confidence: 99%

Characterisation of NcGRA7 and NcSAG4 proteins: Immunolocalisation and their role in the host cell invasion by Neospora caninum tachyzoites

Aguado‐Martínez

Álvarez‐García

Schares

et al. 2010

Acta Parasitologica

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“…This is a protozoan coccidian parasite that structurally resembles and is genetically related to Toxoplasma gondii (Silva et al, 2007). There are two species of Neospora currently recognized: N. caninum which causes clinical disease in dogs, cattle, sheep, equines and many wild animal species, and Neospora hughesi, which has been associated with reproductive losses and myoencephalitis in horses.…”

Section: Neosporosismentioning

confidence: 99%

“…This parasite has been reported to be the most important cause of abortion and neonatal mortality in beef and dairy cattle populations worldwide including Ethiopia (Murray, 2006;Silva et al, 2007;Asmare et al, 2012).…”

Section: Neosporosismentioning

confidence: 99%

Review of common causes of abortion in dairy cattle in Ethiopia

Tulu¹,

Deresa²,

Begna³

et al. 2018

J. Vet. Med. Anim. Health

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Abortion in dairy cattle may be caused by infectious and non-infectious agents. Infectious causes of abortion in dairy cattle include brucellosis, leptospirosis, listeriosis, Q fever, bovine viral diarrhea, mycotic abortion and neosporosis. Non-infectious causes of abortion in dairy cattle are genetic and non-genetic disorder. Risk factors associated with abortion in dairy cattle are genetic, environmental, management, geographical factors and infectious factors. Abortion in dairy cows brings about breeding and productive damages. Abortions cause significant economic loss to dairy farm. These losses can be attributed to loss of replacement calves, reduced milk production, costs of treatment, feeding of animals and premature culling of productive cows and heifers. Diagnosis of bovine abortion includes the collection of a complete history of the case and relevant epidemiological data and collected sample for analysis. However, determining the cause of bovine abortion is difficult as abortions are caused by numerous infectious and noninfectious factors. Status of abortion and breeds affected by abortion in Ethiopia were also reviewed.

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“…Serum samples showing fluorescence at the dilution of 1:50 (cutoff value) were further tittered using twofold serial dilutions (Dubey et al, 1988a(Dubey et al, , 1988b. The entire surface fluorescence of tachyzoite was considered positive (Silva et al, 2007). On each slide, a negative and a positive control were included.…”

Section: Indirect Fluorescent Antibody Testmentioning

confidence: 99%

Seroprevalence of <i>Neospora Caninum</i> in Stray Dogs

Garedaghi¹

2011

American Journal of Animal and Veterinary Sciences

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Problem statement: To investigate anti-Neospora caninum antibodies in stray dogs living in Tabriz city, 100 blood samples were collected. Serum samples were screened for detection of anti-N. caninum IgG antibodies using Indirect Fluorescent Antibody Test (IFAT; ≥50). Antibodies were seen in 31 (31%) of 100 dogs. Approach: The IFAT antibody titers were as follows: 1:50 in 11 dogs, 1:100 in ten dogs, 1:200 in six dogs, 1:400 in one dog, 1:800 in two dogs and 1:1,600 in one dog. There were no significant differences in seroprevalence of Neospora infection between different genders (p>0.05). The seropositive results were increased with age and differences were statistically significant (p<0.05). Results:The results confirm the presence and exposure of stray dogs to N. caninum in Tabriz city and importance of this protozoan as a cause of disease in dogs of the region. Conclusion: Based on the present study, this is the first report on seroprevalence of N. caninum in stray dogs of the Tabriz northwest of Iran. This finding confirms the presence of Neospora infection and the important role of dogs in the region.

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Evaluation of serological tests for the diagnosis of Neospora caninum infection in dogs: Optimization of cut off titers and inhibition studies of cross-reactivity with Toxoplasma gondii

Cited by 76 publications

References 35 publications

Characterisation of NcGRA7 and NcSAG4 proteins: Immunolocalisation and their role in the host cell invasion by Neospora caninum tachyzoites

Characterisation of NcGRA7 and NcSAG4 proteins: Immunolocalisation and their role in the host cell invasion by Neospora caninum tachyzoites

Review of common causes of abortion in dairy cattle in Ethiopia

Seroprevalence of <i>Neospora Caninum</i> in Stray Dogs

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