Evaluation of sampling and storage procedures on preserving the community structure of stool microbiota: A simple at-home toilet-paper collection method

Al, Kait F.; Bisanz, Jordan E.; Gloor, Gregory B.; Reid, Gregor; Burton, Jeremy P.

doi:10.1016/j.mimet.2017.11.014

Cited by 29 publications

(23 citation statements)

References 17 publications

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“…Clinical samples were collected at Victoria Hospital (London, Ontario, Canada) between 2017 and 2018. Gloves used for digital rectal examinations of PC patients were collected and stored at 4°C immediately afterwards for 1-2 days (a convenient storage period and temperature expected to be comparable to immediate freezing 46 ). Subsequently, fecal matter was aseptically transferred from each glove using a single sterile polyester tipped swab (BD, Franklin Lakes, NJ) and stored in a 1.5 mL RNase/DNase-free microcentrifuge tube at −80°C until DNA extraction.…”

Section: Methodsmentioning

confidence: 99%

Abiraterone acetate preferentially enriches for the gut commensal Akkermansia muciniphila in castrate-resistant prostate cancer patients

Daisley¹,

Chanyi²,

Abdur-Rashid³

et al. 2020

Nat Commun

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Abiraterone acetate (AA) is an inhibitor of androgen biosynthesis, though this cannot fully explain its efficacy against androgen-independent prostate cancer. Here, we demonstrate that androgen deprivation therapy depletes androgen-utilizing Corynebacterium spp. in prostate cancer patients and that oral AA further enriches for the health-associated commensal, Akkermansia muciniphila. Functional inferencing elucidates a coinciding increase in bacterial biosynthesis of vitamin K2 (an inhibitor of androgen dependent and independent tumor growth). These results are highly reproducible in a host-free gut model, excluding the possibility of immune involvement. Further investigation reveals that AA is metabolized by bacteria in vitro and that breakdown components selectively impact growth. We conclude that A. muciniphila is a key regulator of AA-mediated restructuring of microbial communities, and that this species may affect treatment response in castrate-resistant cohorts. Ongoing initiatives aimed at modulating the colonic microbiota of cancer patients may consider targeted delivery of poorly absorbed selective bacterial growth agents.

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Section: Methodsmentioning

confidence: 99%

Abiraterone acetate preferentially enriches for the gut commensal Akkermansia muciniphila in castrate-resistant prostate cancer patients

Daisley¹,

Chanyi²,

Abdur-Rashid³

et al. 2020

Nat Commun

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“…A Biomek 3000 laboratory automation workstation was utilized for PCR reagent setup. Amplifications of the V4 region of the 16S rRNA gene were carried out as described previously (71). Processing of DNA samples and DNA sequencing were conducted at the London Regional Genomics Centre at Robarts Research Institute (London, Ontario, Canada).…”

Section: Methodsmentioning

confidence: 99%

Oxalate-Degrading Bacillus subtilis Mitigates Urolithiasis in a Drosophila melanogaster Model

Daisley

Chanyi

et al. 2020

mSphere

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Kidney stone disease is a morbid condition that is increasing in prevalence, with few nonsurgical treatment options. The majority of stones are composed of calcium oxalate. Unlike humans, some microbes can break down oxalate, suggesting that microbial therapeutics may provide a novel treatment for kidney stone patients. This study demonstrated that Bacillus subtilis 168 (BS168) decreased stone burden, improved health, and complemented the microbiota in a Drosophila melanogaster urolithiasis model, while not exacerbating calcium oxalate aggregation or adhesion to renal cells in vitro. These results identify this bacterium as a candidate for ameliorating stone formation; given that other strains of B. subtilis are components of fermented foods and are used as probiotics for digestive health, strain 168 warrants testing in humans. With the severe burden that recurrent kidney stone disease imposes on patients and the health care system, this microbial therapeutic approach could provide an inexpensive therapeutic adjunct.

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“…Results are variable and often contradictory. RNAlater is frequently used to store faecal samples for microbiota analysis (Al et al, 2018;Broquet et al, 2007;Horng et al, 2018;Vlčková et al, 2012;Vogtmann et al, 2017), yet there is evidence that its performance decreases after a period of time at room temperature (Flores et al, 2015), and that the bacterial community profiles differ to those of frozen samples (Choo et al, 2015). Ethanol is also regularly used and has been shown to produce microbial profiles comparable to those obtained with RNAlater (Vogtmann et al, 2017).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

How should we store avian faecal samples for microbiota analyses? Comparing efficacy and cost-effectiveness

Vargas-Pellicer

Watrobska

Knowles

et al. 2019

Journal of Microbiological Methods

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Evaluation of sampling and storage procedures on preserving the community structure of stool microbiota: A simple at-home toilet-paper collection method

Cited by 29 publications

References 17 publications

Abiraterone acetate preferentially enriches for the gut commensal Akkermansia muciniphila in castrate-resistant prostate cancer patients

Abiraterone acetate preferentially enriches for the gut commensal Akkermansia muciniphila in castrate-resistant prostate cancer patients

Oxalate-Degrading Bacillus subtilis Mitigates Urolithiasis in a Drosophila melanogaster Model

How should we store avian faecal samples for microbiota analyses? Comparing efficacy and cost-effectiveness

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