Evaluation of Safety and Protective Efficacy of a waaJ and spiC Double Deletion Korean Epidemic Strain of Salmonella enterica Serovar Gallinarum

Zhang, Junfeng; Shang, Ke; Wei, Bai; Lee, Yea-Jin; Park, Jong-Yeol; Jang, Hyung‐Kwan; Cha, Se-Yeoun; Kang, Min

doi:10.3389/fvets.2021.756123

Cited by 3 publications

(1 citation statement)

References 39 publications

(68 reference statements)

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“…SpiC is an effector protein encoded by SPI-2, which is secreted by the Salmonella type III secretion system (T3SS) and injected into host cells (19,20). It has been demonstrated that deleting spiC gene significantly reduces the virulence of Salmonella in mouse and chicken models (21). The spiC gene deletion strain of Salmonella Pullorum constructed by genetic engineering has been evaluated as a candidate vaccine for S. Pullorum (22,23).…”

Section: Introductionmentioning

confidence: 99%

Safety of the Salmonella enterica serotype Dublin strain Sdu189-derived live attenuated vaccine—A pilot study

Wang

Wang²,

Ge³

et al. 2022

Front. Vet. Sci.

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Salmonella enterica serovar Dublin (S. Dublin) is an important zoonotic pathogen with high invasiveness. In the prevention and control of the Salmonella epidemic, the live attenuated vaccine plays a very important role. To prevent and control the epidemic of S. Dublin in cattle farms, the development of more effective vaccines is necessary. In this study, we constructed two gene deletion mutants, Sdu189ΔspiC and Sdu189ΔspiCΔaroA, with the parental strain S. Dublin Sdu189. The immunogenicity and protective efficacy were evaluated in the mice model. First, both mutant strains were much less virulent than the parental strain, as determined by the 50% lethal dose (LD50) for specific pathogen-free (SPF) 6-week-old female BALB/c mice. Second, the specific IgG antibody level and the expression level of cytokine TNF-α, IFN-γ, IL-4, and IL-18 were increased significantly in the vaccinated mice compared to the control group. In addition, the deletion strains were cleared rapidly from organs of immunized mice within 14 d after immunization, while the parental strain could still be detected in the spleen and liver after 21 d of infection. Compared with the parental strain infected group, no obvious lesions were detected in the liver, spleen, and cecum of the deletion strain vaccinated groups of mice. Immunization with Sdu189ΔspiC and Sdu189ΔspiCΔaroA both provided 100% protection against subsequent challenges with the wild-type Sdu189 strain. These results demonstrated that these two deletion strains showed the potential as live attenuated vaccines against S. Dublin infection. The present study established a foundation for screening a suitable live attenuated Salmonella vaccine.

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Section: Introductionmentioning

confidence: 99%

Safety of the Salmonella enterica serotype Dublin strain Sdu189-derived live attenuated vaccine—A pilot study

Wang

Wang²,

Ge³

et al. 2022

Front. Vet. Sci.

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Prospective lipid-A altered live attenuated Salmonella Gallinarum confers protectivity, DIVA capability, safety and low endotoxicity against fowl typhoid

Hewawaduge

Sivasankar

Lee

2022

Veterinary Microbiology

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The Basis for Variations in the Biofilm Formation by Different Salmonella Species and Subspecies: An In Vitro and In Silico Scoping Study

Sarjit

Cheah

Dykes

2023

Applied Microbiology

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This study examined whether the presence/absence of biofilm-associated genes may indicate the potential for differences in the biofilm formation among the Salmonella species/subspecies. We conducted an in vitro study on the biofilm formation by eighteen Salmonella strains of different species/subspecies. Strains belonging to subspecies enterica were generally poorer biofilm formers than strains belonging to species bongori and subspecies arizonae, diarizonae, and indica. A broader in silico study was subsequently conducted. The presence/absence of 57 biofilm-associated genes was further investigated among 323 Salmonella whole genomes of various species/subspecies. The lpfE gene was present in in 88.2% of subspecies enterica but was absent in ~90.2–100% of other subspecies. The sirA gene was present in 11.8% of subspecies enterica and 2.9% of S. diarizonae genomes while absent in other species/subspecies. The lpfe gene and sirA gene in subspecies enterica negatively correlated with environmental biofilm formation. The csrB gene was present in 71.4% of the S. arizonae and 94.3% of S. diarizonae genomes but absent in other species/subspecies. The absence of csrB in subspecies enterica positively correlated with weaker environmental biofilm formation. This may contribute to subspecies arizonae and diarizonae being better biofilm formers.

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Evaluation of Safety and Protective Efficacy of a waaJ and spiC Double Deletion Korean Epidemic Strain of Salmonella enterica Serovar Gallinarum

Cited by 3 publications

References 39 publications

Safety of the Salmonella enterica serotype Dublin strain Sdu189-derived live attenuated vaccine—A pilot study

Safety of the Salmonella enterica serotype Dublin strain Sdu189-derived live attenuated vaccine—A pilot study

Prospective lipid-A altered live attenuated Salmonella Gallinarum confers protectivity, DIVA capability, safety and low endotoxicity against fowl typhoid

The Basis for Variations in the Biofilm Formation by Different Salmonella Species and Subspecies: An In Vitro and In Silico Scoping Study

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