Evaluation of Rheum Turkestanicum in Hexachlorobutadien-Induced Renal Toxicity

Abstract

Hexachlorobutadien is nephrotoxic agent in rodents. The mechanism of toxicity includes generation of free radicals, depletion of thiol groups and production of toxic metabolites. Antioxidant compounds may reduce HCBD-nephrotoxicity. In this research we investigated the effect of Rheum turkeatanicum extract against HCBD-toxicity. The animals were divided to 4 groups which were including control (saline, 1 mL/kg), HCBD (100 mg/kg) and treatment groups which received extract at doses 100 and 200 mg/kg. The extract were administered as intraperitoneally (i.p.) 1 h before HCBD injection (i.p.). The animals were anesthetized by ether, 24 h after HCBD administration. The results showed elevation of serum creatinine, serum urea, urinary protein, urinary glucose, malondialdehyde levels in kidney and reduction of thiol in kidney by HCBD. The histopathological studies showed that there was apoptosis and necrosis in HCBD treated groups. Administration of R.turkestanicum reduced HCBD toxicity. The extract reduced hitopathological changes in kidney. It may be concluded that the nephroprotective effect of extract may be due to different mechanisms such as antioxidant activity or by decreasing the toxic metabolites of HCBD or inhibition of enzymes which are involved in the bioactivation of HCBD such as glutathione-S-transferase (GST) or cysteine-S-conjugate β-lyase.

Protective Effects of Rheum Turkestanicum Janischagainst Diethylnitrosamine-Induced Hepatocellular Carcinoma in Rats

“…The R. turkestanicum extract could restore these parameters, which was in agreement with antioxidant and hepatoprotective effects of this plant reported by other studies [9,12,35]. Also, there are reports that protective effects of R. turkestanicum against organ toxicity induced by doxorubicin [36], gentamicin [37], HgCl 2 [38], and hexachlorobutadiene [37] is mediated through its antioxidant activity.…”

Cardioprotective Effect of Rheum turkestanicum Against Doxorubicin-Induced Toxicity in Rats

“…Moreover, previous research has also reported that doxorubicin elevates ALT ( Barakat et al, 2018 ); our findings support these previous ones of doxorubicin-induced clinical enzyme alterations. Our findings also support that R. turkestanicum protects against doxorubicin-induced cardiotoxicity by restoring cellular antioxidant defenses and as an antioxidant in various cells and toxicity models ( Hosseini et al, 2018 ; Boroushaki et al, 2019a ; Boroushaki et al, 2019b ; Hosseini et al, 2020 ). The protection by the plant is also associated with improvements in LDH and creatinine phosphokinase activities ( Hosseini et al, 2017 ; Hossini et al, 2022 ).…”

The effect of hydro-alcoholic extract ofRheum Turkestanicum Roots against oxidative stress in endothelial cells

“…R. turkestanicum reduced doxorubicin-induced toxicity in cardiomyocytes (H9C2 cells),[ 12 ] glutamate toxicity in PC12 cells. [ 18 ] The protective effects of this herb against toxic agents such as gentamicin,[ 14 ] mercuric chloride,[ 15 ] hexachlorbutadien,[ 16 ] and cisplatin[ 13 ] have been also demonstrated. Another study showed that R.turkestanicum prevented cardiac injury following STZ-induced diabetic in rats via attenuation of lactate dehydrogenase and creatine phosphokinase.…”

“…[ 11 ] Recent studies have revealed a protective effect of this plant against toxic agents, such as doxorubicin,[ 12 ] cisplatin,[ 13 ] gentamicin,[ 14 ] mercuric chloride,[ 15 ] and hexachlorobutadien. [ 16 ] Antioxidant activity of some plants of Rheum genus has been attributed to the presence of bioactive components that scavenge free radicals (IC 50 value in DPPH assay: 2.8-11.8 μM). [ 17 ] R. turkestanicum counter acted hexachloro butadien and mercuric chloride-induced nephrotoxicity through its antioxidant properties indicated by attenuated lipid peroxidation and increased total thiol content.…”