Evaluation of real-time PCR of patient pleural effusion for diagnosis of tuberculosis

Rosso, Franciele; Michelon, Candice Tosi; Sperhacke, Rosa Dea; Verza, Mirela; Olival, Liliane; Conde, Marcus Barreto; Guerra, Renata Leborato; Zaha, Arnaldo; Rossetti, Maria Lúcia Rosa

doi:10.1186/1756-0500-4-279

Cited by 32 publications

(33 citation statements)

References 21 publications

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“…Real-time PCR is a novel and robust assay primarily used to quantify the nucleic acid molecules in EPTB specimens (Baba et al, 2008;Rosso et al, 2011). The main advantages of real-time PCR are shortened turnaround time, quantification of bacterial load and automation of the procedure that reduces hands-on time and decreased risk of crosscontamination (Kalantri et al, 2011;Rosso et al, 2011).…”

Section: Naa Testsmentioning

confidence: 99%

Diagnosis of extrapulmonary tuberculosis by PCR

Mehta

Raj

Singh

et al. 2012

FEMS Immunol Med Microbiol

147

117

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During the last two decades, the resurgence of tuberculosis (TB) has been documented in both developed and developing nations, and much of this increase in TB burden coincided with human immunodeficiency virus (HIV) epidemics. Since then, the disease pattern has changed with a higher incidence of extrapulmonary tuberculosis (EPTB) as well as disseminated TB. EPTB cases include TB lymphadenitis, pleural TB, TB meningitis, osteoarticular TB, genitourinary TB, abdominal TB, cutaneous TB, ocular TB, TB pericarditis and breast TB, although any organ can be involved. Diagnosis of EPTB can be baffling, compelling a high index of suspicion owing to paucibacillary load in the biological specimens. A negative smear for acid-fast bacilli, lack of granulomas on histopathology and failure to culture Mycobacterium tuberculosis do not exclude the diagnosis of EPTB. Novel diagnostic modalities such as nucleic acid amplification (NAA) can be useful in varied forms of EPTB. This review is primarily focused on the diagnosis of several clinical forms of EPTB by polymerase chain reaction (PCR) using different gene targets.

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Section: Naa Testsmentioning

confidence: 99%

Diagnosis of extrapulmonary tuberculosis by PCR

Mehta

Raj

Singh

et al. 2012

FEMS Immunol Med Microbiol

147

117

View full text Add to dashboard Cite

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“…It can be explained by that, in country like Bangladesh where the prevalence of tuberculosis is very high, the possibility of infection with tuberculosis may coexist 13 . This could be also due to cross contamination during tube to tube transfer 14 .…”

Section: Discussionmentioning

confidence: 99%

TRC4 Gene Based PCR Assay in Diagnosis of Tuberculous Meningitis

Sattar

Setu

Saleh

et al. 2017

Bangladesh J Med Microbiol

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The prevalence of Tuberculous meningitis remains largely underestimated due to nonspecific clinical manifestation at early stages. A total of 20 CSF samples were studied from clinically suspected cases of tuberculous meningitis. All the samples were processed for ZN staining, TB culture on LJ media and TB-PCR using IS6110 and TRC4 primers by standard protocols. Of the total 20 CSF samples, 12 cases were diagnosed as TB meningitis. Most of the tuberculous meningitis cases were found positive by PCR using TRC4 primers (83.33%) followed by IS6110 primer (66.67%) and culture on L-J media(8.33%). None were found positive by ZN staining. TB-PCR usingTRC4 primer showed higher positivity than using IS6110 in detecting tuberculous meningitis, since some strains of MTB may lack the IS6110 element in their genome. PCR assay using TRC4 primer is superior in diagnosing tuberculous meningitis. This study was aimed to evaluate the diagnostic potentials of CSF polymerase chain reaction (PCR) by using TRC4 and IS6110 primers. Further the results were also compared with culture on Lowenstein-Jensen (LJ) media and AFB smear by ZeihlNelson (ZN) staining.

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“…In our study, the NPV of PCR for MTB was low in comparison to ADA which has been found in other studies also. 20 Hence, a negative result of PCR for MTB is not useful in excluding the diagnosis of tuberculosis in a pleural effusion.…”

Section: Discussionmentioning

confidence: 99%

The advantage of PCR for MTB in comparison to ADA in diagnosing tubercular pleural effusion

et al. 2018

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Background: Tuberculosis continues to be an important health problem globally. The bacteriological confirmation of diagnosis in extrapulmonary tuberculosis patients is more difficult because most of the cases of extrapulmonary tuberculosis are paucibacillary in nature. In this study we have compared the pleural fluid ADA levels with PCR for MTB in pleural fluid to confirm the diagnosis of tuberculosis in the pleural fluid.Methods: The study was done over two years and a total of 106 patients with a clinico-radiological diagnosis of pleural effusion were enrolled for the study. The pleural fluid was aspirated and examined for total cell count, differential cell count, protein, sugar, ADA and PCR for MTB.A CT Thorax was done in all the 106 patients of pleural effusion and underlying consolidation along with pleural effusion was found in 60 patients.Results: The pleural fluid was exudative in nature in all the patients. 90 patients (84.9%) had lymphocyte predominant pleural effusion while 16 patients (15.1%) had neutrophil predominant pleural effusion. The overall sensitivity of ADA in all the cases of pleural effusion was 85.2% while the overall sensitivity of PCR for MTB in all the cases of pleural effusion was 51.1%. However, in the 60 patients of pleural effusion with underlying lung consolidation, the overall sensitivity of ADA was 69.1% while the overall sensitivity of PCR for MTB was 92.8% for diagnosing tubercular pleural effusion.Conclusions: PCR for MTB is a useful test along with ADA for diagnosing tubercular pleural effusion. PCR for MTB is especially useful in the diagnosis of tubercular pleural effusion in patients with underlying lung consolidation.

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Evaluation of real-time PCR of patient pleural effusion for diagnosis of tuberculosis

Cited by 32 publications

References 21 publications

Diagnosis of extrapulmonary tuberculosis by PCR

Diagnosis of extrapulmonary tuberculosis by PCR

TRC4 Gene Based PCR Assay in Diagnosis of Tuberculous Meningitis

The advantage of PCR for MTB in comparison to ADA in diagnosing tubercular pleural effusion

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