Evaluation of Polymorphisms at Heat Shock Protein 90 Gene by High Resolution Melting Assays for Potential Heat Tolerance among Nigerian Zebu Cattle Breeds

Abstract

Heat Shock Protein (HSP) 90 gene is a member of HSPs subfamily that act as molecular chaperons whenever animals come under thermal stress. The genes fulfill essential roles of providing cellular protection, immune response, protein synthesis, protein folding and unfolding, protection from cellular stress, inhibitory apoptosis and adaptation. This study was designed to analyze polymorphisms of HSP 90 and to evaluate their influence on heat tolerance among selected Nigerian zebu. The polymorphisms were also used to determine genetic relationship among the animals. About 450 bp of bovine HSP 90 including part of coding region in exon 3 was sequenced in 90 DNA samples representing four Nigerian zebu namely White Fulani (WF), Sokoto Gudali (SG), Red Bororo (RB) and Ambala (AM). Sequencing was done using an automated ABI-DNA Sequencer. Editing was accomplished using chromatogram analyses on SeqMan Ngen Tool. Rooted phylogenetic tree was constructed using MEGA 5.2 software. In total, 11 genetic variants were determined. Five of these (PRP, RED, ORG, LMN and YLO) were major variants detected in over 70% of the samples. Six (6) were classified as minor variants detected in two breeds or less and in 29.1% of the samples. The GRN and NBL were only detected in RB and SG breeds respectively. We found a shared homology and common ancestral lineage among the breeds. Furthermore, the genetic structure of Nigerian zebu has a common clade architecture to those of goats, sheep, yak, buffalo, camel, horse and other taurines. The gene is conserved among wide range of animals and as such it can serve as one of bio-markers for selection and breeding programmes for thermotolerance in wide range of livestock animals under thermal stress. The variant groups could be further interrogated for possible specific effects on thermotolerance performance of zebu in hot tropical environments.

Genetic polymorphisms of the 5' untranslated regions of the HSP70 gene in Indonesian cattle populations

“…Heat stress has become a significant issue in climate change because it directly impacts the adaptability and survivability of farm animals to thermal assault. The HSP genes such as HSP70 and HSP90 are members of HSPs subfamily (molecular chaperone families) known to be highly expressed under stressful environmental and physiological conditions [27]. This study analyzed the polymorphisms of the 5' UTR of the HSP70 gene in the seven cattle populations in Indonesia.…”

Potentials of single nucleotide polymorphisms and genetic diversity studies at HSP90AB1 gene in Nigerian White Fulani, Muturu, and N’Dama cattle breeds

“…Heat shock proteins are group of proteins induced by thermal assault/ heat shock either facultatively or constitutively animals and humans are exposed to assault of thermal conditions (Onasanya et al 2021). Single nucleotide polymorphisms of HSPs 70 and 90 genes have been extensively characterized among Nigerian breeds of cattle particularly White Fulani, Sokoto Gudali, Red Bororo and Ambala and it was reported that Nigerian cattle breeds at these loci are highly polymorphic and diverse where detected SNP genotypes were found to have positive biological functions on the thermotolerance potentials (Onasanya et al 2021;Onasanya et al 2020 a, b;Onasanya et al 2019). The most prominent members of this group are a class of functionally-related proteins that are involved in the folding and unfolding of other proteins (De-Maio, 1999).…”

Potentials of Single Nucleotide Polymorphisms and Genetic Diversity Studies at HSP90AB1 Gene in Nigerian White Fulani, Muturu and N’Dama Cattle Breeds

“…Heat shock proteins are group of proteins induced by thermal assault/ heat shock either facultatively or constitutively animals and humans are exposed to assault of thermal conditions (Onasanya et al 2021). Single nucleotide polymorphisms of HSPs 70 and 90 genes have been extensively characterized among Nigerian breeds of cattle particularly White Fulani, Sokoto Gudali, Red Bororo and Ambala and it was reported that Nigerian cattle breeds at these loci are highly polymorphic and diverse where detected SNP genotypes were found to have positive biological functions on the thermotolerance potentials (Onasanya et al 2021;Onasanya et al 2020 a, b;Onasanya et al 2019). The most prominent members of this group are a class of functionally-related proteins that are involved in the folding and unfolding of other proteins (De-Maio, 1999).…”