Evaluation of omental inflammatory response with P-/E-selectin levels and histopathologic findings in experimental model

Aslan, Mustafa; Boybeyi, Özlem; Soyer, Tutku; Şenyücel, Mine; Ayva, Ebru Sebnem; Kısa, Üçler; Cesur, Özkan; Çakmak, Murat

doi:10.1016/j.jpedsurg.2012.06.024

Cited by 4 publications

(3 citation statements)

References 9 publications

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“…In cecum perforation studies[1-3] the immediate post-perforation threat is rarely studied, particularly regarding: the rapid disappearance of blood vessels in the cecum serosa that are instantly emptied and thereby “disappear”, perforation defect enlargement, bleeding and fluid leakage, increased oxidative stress and disturbed nitric oxide (NO) levels in cecum tissue. Likewise, the possible effect of cytoprotective agents, known to act as a class on endothelium maintenance in the gastrointestinal tract (and, thereby, mucosal maintenance)[4-9], especially the stable gastric pentadecapeptide BPC 157 as a cytoprotective agent rapidly acting on endothelium integrity maintenance[10-19], has been not investigated.…”

Section: Introductionmentioning

confidence: 99%

Counteraction of perforated cecum lesions in rats: effects of pentadecapeptide BPC 157, L-NAME and L-arginine

Drmic¹,

Samara²,

Vidović³

et al. 2018

WJG

113

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AIMTo study the counteraction of perforated cecum lesion using BPC 157 and nitric oxide (NO) system agents.METHODSAlongside with the agents’ application (after 1 min, medication (/kg, 10 mL/2 min bath/rat) includes: BPC 157 (10 μg), L-NAME (5 mg), L-arginine (100mg) alone or combined, and saline baths (controls)) on the rat perforate cecum injury, we continuously assessed the gross reappearance of the vessels (USB microcamera) quickly propagating toward the defect at the cecum surface, defect contraction, bleeding attenuation, MDA- and NO-levels in cecum tissue at 15 min, and severity of cecum lesions and adhesions at 1 and 7 d.RESULTSPost-injury, during/after a saline bath, the number of vessels was significantly reduced, the defect was slightly narrowed, bleeding was significant and MDA-levels increased and NO-levels decreased. BPC 157 bath: the vessel presentation was markedly increased, the defect was noticeably narrowed, the bleeding time was shortened and MDA- and NO-levels remained normal. L-NAME: reduced vessel presentation but not more than the control, did not change defect and shortened bleeding. L-arginine: exhibited less vessel reduction, did not change the defect and prolonged bleeding. In combination, mutual counteraction occurred (L-NAME + L-arginine) or the presentation was similar to that of BPC 157 rats (BPC 157 + L-NAME; BPC 157 + L-arginine; BPC 157 + L-NAME + L-arginine), except the defect did not change. Thereby at day 1 and 7, saline, L-NAME, L-arginine and L-NAME + L-arginine failed (defect was still open and large adhesions present).CONCLUSIONThe therapeutic effect was achieved with BPC 157 alone or in combination with L-NAME and L-arginine as it was able to consolidate the stimulating and inhibiting effects of the NO-system towards more effective healing recruiting vessels.

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Section: Introductionmentioning

confidence: 99%

Counteraction of perforated cecum lesions in rats: effects of pentadecapeptide BPC 157, L-NAME and L-arginine

Drmic¹,

Samara²,

Vidović³

et al. 2018

WJG

113

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“…The E-selectin, CSF2 and PTX3 were the top 3 of the most upregulated genes. The E-selectin is important for leukocyte accumulation in inflammatory responses and PTX3 can amplify the immune response ( Aslan et al, 2012 ; Ketter et al, 2016 ). In our previous research, a different immunogenicity of the inactivated COVID-19 vaccine produced by the F8 strain was found compared with that produced by the 8X strain ( Sun et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptomic analyzes of human lung epithelial cells infected with wild-type SARS-CoV-2 and its variant with a 12-bp missing in the E gene

Sun

Sun²,

Zhu³

et al. 2023

Front. Microbiol.

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The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel coronavirus that caused a global outbreak of coronavirus disease 2019 (COVID-19) pandemic. To elucidate the mechanism of SARS-CoV-2 replication and immunogenicity, we performed a comparative transcriptome profile of mRNA and long non-coding RNAs (lncRNAs) in human lung epithelial cells infected with the SARS-CoV-2 wild-type strain (8X) and the variant with a 12-bp deletion in the E gene (F8). In total, 3,966 differentially expressed genes (DEGs) and 110 differentially expressed lncRNA (DE-lncRNA) candidates were identified. Of these, 94 DEGs and 32 DE-lncRNAs were found between samples infected with F8 and 8X. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyzes revealed that pathways such as the TNF signaling pathway and viral protein interaction with cytokine and cytokine receptor were involved. Furthermore, we constructed a lncRNA-protein-coding gene co-expression interaction network. The KEGG analysis of the co-expressed genes showed that these differentially expressed lncRNAs were enriched in pathways related to the immune response, which might explain the different replication and immunogenicity properties of the 8X and F8 strains. These results provide a useful resource for studying the pathogenesis of SARS-CoV-2 variants.

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“…The endothelium lining the omental capillaries and the mesothelium overlying milky spots are adapted to facilitate transmigration of leucocytes. When exposed to a foreign substance, inflammatory factors are activated, leading to recruitment of inflammatory cells within the peritoneal cavity (28). The idea of using an omental flap was to take advantage of its pro-inflammatory reactions.…”

Section: Omental Activitiesmentioning

confidence: 99%

Left Ventricular Assist Pump Pocket Infection: Conservative Treatment Strategy for Destination Therapy Candidates

et al. 2017

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Evaluation of omental inflammatory response with P-/E-selectin levels and histopathologic findings in experimental model

Cited by 4 publications

References 9 publications

Counteraction of perforated cecum lesions in rats: effects of pentadecapeptide BPC 157, L-NAME and L-arginine

Counteraction of perforated cecum lesions in rats: effects of pentadecapeptide BPC 157, L-NAME and L-arginine

Comparative transcriptomic analyzes of human lung epithelial cells infected with wild-type SARS-CoV-2 and its variant with a 12-bp missing in the E gene

Left Ventricular Assist Pump Pocket Infection: Conservative Treatment Strategy for Destination Therapy Candidates

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