Evaluation of microbial population and functional genes during the bioremediation of petroleum-contaminated soil as an effective monitoring approach

Detection and Quantification of Sulfate-Reducing and Polycyclic Aromatic Hydrocarbon-Degrading Bacteria in Oilfield Using Functional Markers and Quantitative PCR

“…The authors reported spatial variation in the density and diversity of naphthalene dioxygenase genes and the gene abundance correlated positively with the level of the total organic carbon and aromatic hydrocarbons. Also in a recent study, Shahi et al [19] applied Illumina sequencing and qPCR of functional genes involved in degradation of alkanes and aromatics (alkB, phnAc and nah) to monitor bioremediation of petroleum-contaminated soil.…”

Shift in microbial group during remediation by enhanced natural attenuation (RENA) of a crude oil-impacted soil: a case study of Ikarama Community, Bayelsa, Nigeria

“…Further, Tauler et al (2016) also reported 80% proteobacteria phylum dominance during PAH-remediation studies in an enriched soil consortium. Nutrient addition during the RENA process stimulated these bacterial groups (Gram-negative), and boost their competitive advantage (Shahi et al 2016) and metabolic capabilities (Peresutti et al 2003) over other bacterial communities in soil polluted with hydrocarbons. This resulted in a shift from mixed population of Gram-positive and negative bacteria to only Gram-negative bacteria at the end of the RENA treatment.…”

“…With regards to microbial population in a polluted environment, there are two major approaches to improve microbial activities on pollutant utilization as carbon source: bioaugmentation and biostimulation (Azubuike et al 2016). Despite the limitations of bioaugmentation (Megharaj et al 2011;Gao et al 2014;Shahi et al 2016), it can be used to remediate sites polluted with recalcitrant pollutants (Philp and Atlas 2005). Nevertheless, biostimulation is a more cost-effective and practical approach to bioremediation (Simpanen et al 2016).…”

The effects of white-rot fungi Trametes versicolor and Bjerkandera adusta on microbial community structure and functional genes during the bioaugmentation process following biostimulation practice of petroleum contaminated soil

“…TOC removal in selected soil samples by T. versicolor and B. adusta was measured. TOC/TPH correlation is confirmed (around 90%) for the studied soil (Shahi et al, 2016) this is why we used TOC analysis for evaluation of the biodegradation manner. Wheat grains were used as support for inoculums and a device for soil colonization.…”

“…The value of this technique as a precise method for analysis of dominant species has been previously confirmed through different works (Yadav et al, 2015;You et al, 2015). Real time PCR method as an automated, sensitive and fast tool for monitoring of bioremediation process has been also tested successfully in our previous work (Shahi et al, 2016;Aydin et al, 2015). The use of Illumina MiSeq method along with real time PCR can be suggested as an effective method for presentation of the real feature of microbial mutual interactions during bioremediation process.…”