Evaluation of MALDI-ToF as a method for the identification of bacteria in the veterinary diagnostic laboratory

Randall, Luke; Lemma, Fabrizio; Koylass, Mark S.; Rogers, Jon; Ayling, Roger D.; Worth, Danny; Klita, Monika; Steventon, Andrew; Line, K.; Wragg, Peter; Muchowski, Jakub; Kostrzewa, Markus; Whatmore, Adrian M.

doi:10.1016/j.rvsc.2015.05.018

Cited by 74 publications

(56 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…) or by MALDI ToF MS (Randall et al . ). Selected Salmonella were identified to serotype level using the Kaufmann–White–Le Minor scheme (Anonymous, ).…”

Section: Methodsmentioning

confidence: 97%

Longitudinal study on the occurrence in pigs of colistin‐resistant Escherichia coli carrying mcr‐1 following the cessation of use of colistin

et al. 2018

View full text Add to dashboard Cite

show abstract

“…) or by MALDI ToF MS (Randall et al . ). Selected Salmonella were identified to serotype level using the Kaufmann–White–Le Minor scheme (Anonymous, ).…”

Section: Methodsmentioning

confidence: 97%

Longitudinal study on the occurrence in pigs of colistin‐resistant Escherichia coli carrying mcr‐1 following the cessation of use of colistin

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Representative isolates presumptively identified as E. coli , Salmonella , or Enterococcus were subcultured to purity and then identified to the species level by MALDI-TOF MS (60). DNA extracts were prepared from these isolates using Prepman Ultra (Life Technologies, Inc.) from overnight culture at 37°C on blood agar.…”

Section: Methodsmentioning

confidence: 99%

An In Vitro Chicken Gut Model Demonstrates Transfer of a Multidrug Resistance Plasmid from Salmonella to Commensal Escherichia coli

Card

Cawthraw

Nuñéz-García

et al. 2017

mBio

View full text Add to dashboard Cite

The chicken gastrointestinal tract is richly populated by commensal bacteria that fulfill various beneficial roles for the host, including helping to resist colonization by pathogens. It can also facilitate the conjugative transfer of multidrug resistance (MDR) plasmids between commensal and pathogenic bacteria which is a significant public and animal health concern as it may affect our ability to treat bacterial infections. We used an in vitro chemostat system to approximate the chicken cecal microbiota, simulate colonization by an MDR Salmonella pathogen, and examine the dynamics of transfer of its MDR plasmid harboring several genes, including the extended-spectrum beta-lactamase blaCTX-M1. We also evaluated the impact of cefotaxime administration on plasmid transfer and microbial diversity. Bacterial community profiles obtained by culture-independent methods showed that Salmonella inoculation resulted in no significant changes to bacterial community alpha diversity and beta diversity, whereas administration of cefotaxime caused significant alterations to both measures of diversity, which largely recovered. MDR plasmid transfer from Salmonella to commensal Escherichia coli was demonstrated by PCR and whole-genome sequencing of isolates purified from agar plates containing cefotaxime. Transfer occurred to seven E. coli sequence types at high rates, even in the absence of cefotaxime, with resistant strains isolated within 3 days. Our chemostat system provides a good representation of bacterial interactions, including antibiotic resistance transfer in vivo. It can be used as an ethical and relatively inexpensive approach to model dissemination of antibiotic resistance within the gut of any animal or human and refine interventions that mitigate its spread before employing in vivo studies.

show abstract

“…Following isolation, identification of M. bovis colonies traditionally uses antibody‐based tests such as growth inhibition, immunofluorescence or dot immunobinding (Miles & Nicholas, ), but species‐specific PCRs are widely used. PCR amplification and sequencing of the 16S rRNA gene from colony‐derived material may also be used for species identification, as can the more rapid technique of MALDI‐TOF mass spectrometry (Pereyre et al., ; Randall et al., ), which is being increasingly implemented in clinical diagnostic laboratories. Although culture is the primary method for mycoplasma identification, it is time‐consuming, laborious and must satisfy the fastidious nutritional requirements of the organism.…”

Section: The Causative Organismmentioning

confidence: 99%

Gap analysis ofMycoplasma bovisdisease, diagnosis and control: An aid to identify future development requirements

Calcutt

Lysnyansky

Sachse

et al. 2018

Transbound Emerg Dis

View full text Add to dashboard Cite

There is a worldwide problem of disease caused by Mycoplasma (M.) bovis in cattle; it has a significant detrimental economic and animal welfare impact on cattle rearing. Infection can manifest as a plethora of clinical signs including mastitis, pneumonia, arthritis, keratoconjunctivitis, otitis media and genital disorders that may result in infertility and abortion. Current diagnosis and control information are reviewed and analysed to identify gaps in knowledge of the causative organism in respect of the disease pathology, diagnosis and control methods. The main considerations are as follows: no vaccines are commercially available; antimicrobial resistance is increasing; diagnostic and antimicrobial sensitivity testing needs to be improved; and a pen-side test would facilitate more rapid diagnosis and implementation of treatment with antimicrobials. More data on host susceptibility, stress factors, immune response and infectious dose levels are required. The impact of asymptomatic carriers, M. bovis survival in the environment and the role of wildlife in transmitting the disease also needs investigation. To facilitate development of vaccines, further analysis of more M. bovis genomes, its pathogenic mechanisms, including variable surface proteins, is required, along with reproducible disease models.

show abstract

Evaluation of MALDI-ToF as a method for the identification of bacteria in the veterinary diagnostic laboratory

Cited by 74 publications

References 44 publications

Longitudinal study on the occurrence in pigs of colistin‐resistant Escherichia coli carrying mcr‐1 following the cessation of use of colistin

Longitudinal study on the occurrence in pigs of colistin‐resistant Escherichia coli carrying mcr‐1 following the cessation of use of colistin

An In Vitro Chicken Gut Model Demonstrates Transfer of a Multidrug Resistance Plasmid from Salmonella to Commensal Escherichia coli

Gap analysis ofMycoplasma bovisdisease, diagnosis and control: An aid to identify future development requirements

Contact Info

Product

Resources

About