Evaluation of industrial <scp><i>S</i></scp><i>accharomyces cerevisiae</i> strains as the chassis cell for second‐generation bioethanol production

Li, Hongxing; Wu, Meiling; Xu, Lili; Hou, Jinjun; Guo, Tao; Bao, Xiaoming; Shen, Yufei

doi:10.1111/1751-7915.12245

Cited by 48 publications

(60 citation statements)

References 35 publications

(51 reference statements)

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“…Considering stress conditions involved in the fermentation processes for the production of 2G ethanol (Costa et al, 2014;Li et al, 2015), the sixteen isolates that showed tolerance to ethanol and were selected had different degrees of tolerance (Table 2), but none of those tolerated all the conditions imposed. This information is corroborated by Li et al (2015), where none of the five strains of S. cerevisiae tolerated all …”

Section: Discussionmentioning

confidence: 99%

“…The conditions tested respectively were: Glucose (35% and 50%), hyperosmotic stresses (10 and 16% KCl), acetic acid (0.5 and 1%), temperatures of 42 and 50°C, vanillin (6 and 8 μmol.L -1 ), furfural (0.25, 0.5, 1.0 and 1.2 g.L -1 ) and oxidative stress. Also concentrations of cellobiose 1% and arabinose 1% (Ali and Khan, 2014;Li et al, 2015) were tested. For this, the strains were previously cultured overnight in YEPD agar medium (composition/L: 10 g yeast extract, 20 g peptone, 20 g dextrose, and 20 g agar, 1000 mL distilled water) and washed twice with sterile water.…”

Section: Tolerance Testsmentioning

confidence: 99%

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Efficient production of second generation ethanol and xylitol by yeasts from Amazonian beetles (Coleoptera) and their galleries

Souza¹,

Valentim²,

Nogueira³

et al. 2017

Afr. J. Microbiol. Res.

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Beetles of the Passalidae family live and feed on decaying wood and their guts are richly colonized by yeasts. The goal of this research was to prospect xylolytic yeasts with potential for the production of second-generation bioethanol. Therefore, 83 specimens of beetles belonging to the Passalidae and the Scarabaeidae families were collected in the Amazonian rainforest in Itacoatiara -AM, Brazil. Morphological differences of the beetles were identified and 25 chosen specimens were dissected. Yeasts from galleries inhabited by beetles and from insect guts were isolated. Isolates were previously selected through tolerance tests for temperature, ethanol and xylose assimilation capacity. Those isolates were then submitted to a panel of conditions related to ethanol production. The ethanol production reached 24.70 g.L -1 and the xylitol production reached 21.66 g.L -1. One of the isolates with a promising profile was identified as Spathaspora roraimanensis and six as Spathaspora passalidarum. Three isolates showed to be more promising and, curiously, all came from the gut of the species Popilius marginatus (Percheron, 1835). In plate testing, however, the isolates obtained from galleries showed a greater capacity to assimilate xylose. As reported in this field of study, no isolate tolerated all conditions tested. Wild isolates with this profile may be used for testing larger-scale ethanol production, genetic engineering, or evolutionary techniques.

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Section: Discussionmentioning

confidence: 99%

Section: Tolerance Testsmentioning

confidence: 99%

Efficient production of second generation ethanol and xylitol by yeasts from Amazonian beetles (Coleoptera) and their galleries

Souza¹,

Valentim²,

Nogueira³

et al. 2017

Afr. J. Microbiol. Res.

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“…Ethanol fermentation strain Saccharomyces cerevisiae XH7 was an engineered strain from the wild diploid Saccharomyces cerevisiae BSIF (Li et al 2015). The strain was cultured in YPD medium containing 20 g/L of glucose, 20 g/L of peptone, and 10 g/L of yeast extract.…”

Section: Strains and Mediummentioning

confidence: 99%

Industrial cellulase performance in the simultaneous saccharification and co-fermentation (SSCF) of corn stover for high-titer ethanol production

Zhang

Bao

2017

Bioresour. Bioprocess.

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Background: Cellulase enzymes contribute to the largest portion of operation cost on production of cellulosic ethanol. The industrial cellulases available on the industrial enzyme market from different makers and sources vary significantly in hydrolysis and ethanol, and finally lead to the changes of enzyme cost. Therefore, the selection of the proper industrial cellulase enzymes for commercial-scale production of cellulosic ethanol is crucially important in terms of high performance and cost reduction. Results:In this study, three major cellulase enzyme products available on the Chinese industrial enzyme market were selected and evaluated as the biocatalysts for the biorefining process of lignocellulose biomass into high-titer ethanol. The cellulase enzymes included Cellic CTec 2.0 from Novozymes (Beijing), and LLC 4 from Vland (Qingdao), as well as # 7 from an industrial enzyme maker. The detailed assays on the filter paper activity, the cellobiase activity, and the total protein contents of the enzymes were conducted according to the standard protocols. When the cellulase enzymes were applied to the practical hydrolysis and ethanol-fermentation operation under the conditions of high solids loading and low range of cellulase dosage, the hydrolysis yield shows the significant difference, and the difference was narrowed in the final ethanol yield. Conclusions:The commercially available cellulase enzymes showed different performances in the activities, the cellulose hydrolysis yield, and the ethanol fermentation yields based on the protein dosage per gram of cellulose of corn stover. In general, the industrial cellulase products give satisfactory performance and can be applied for the practical cellulosic ethanol production on commercial scale.

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“…The growth of most robust microbe for fermentation, Saccharomyces cerevisiae, was inhibited by compounds resulted from lignocelullosic hydrolisate such as aldehydes, ketones, phenols and organic acids (Palmqvist and Hahn-Hagerdal 2000;Li et al 2015). These inhibitory aldehyde compounds have been given more attention during the last two decades due to their toxicity to the fermentative microbe's cells.…”

Section: Introductionmentioning

confidence: 99%

INHIBITION OF THE GROWTH OF TOLERANT YEAST Saccharomyces cerevisiae STRAIN I136 BY A MIXTURE OF SYNTHETIC INHIBITORS

Riyanti¹,

Listanto²

2017

Indones. J. Agric. Sci.

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Biomass from lignocellulosic wastes is a potential source for biobased products. However, one of the constraints in utilization of biomass hydrolysate is the presence of inhibitors. Therefore, the use of inhibitor-tolerant microorganisms in the fermentation is required. The study aimed to investigate the effect of a mixture of inhibitors on the growth of Saccharomyces cerevisiae strain I136 grown in medium containing synthetic inhibitors (acetic acid, formic acid, furfural, 5-hydroxymethyl furfural/5-HMF, and levulinic acid) in four different concentrations with a mixture of carbon sources, glucose (50 g.l -1 ) and xylose (50 g.l -1 ) at 30 o C. The parameters related to growth and fermentation products were observed. Results showed that the strain was able to grow in media containing natural inhibitors (BSL medium) with µmax of 0.020/h. Higher level of synthetic inhibitors prolonged the lag phase, decreased the cell biomass and ethanol production, and specific growth rate. The strain could detoxify furfural and 5-HMF and produced the highest ethanol (Y(p/s) of 0.32 g.g -1 ) when grown in BSL. Glucose was utilized as its level decreased in a result of increase in cell biomass, in contrast to xylose which was not consumed. The highest cell biomass was produced in YNB with Y (x/s) value of 0.25 g.g -1 . The strain produced acetic acid as a dominant side product and could convert furfural into a less toxic compound, hydroxyl furfural. This robust tolerant strain provides basic information on resistance mechanism and would be usefull for bio-based cell factory using lignocellulosic materials.

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Evaluation of industrial Saccharomyces cerevisiae strains as the chassis cell for second‐generation bioethanol production

Cited by 48 publications

References 35 publications

Efficient production of second generation ethanol and xylitol by yeasts from Amazonian beetles (Coleoptera) and their galleries

Efficient production of second generation ethanol and xylitol by yeasts from Amazonian beetles (Coleoptera) and their galleries

Industrial cellulase performance in the simultaneous saccharification and co-fermentation (SSCF) of corn stover for high-titer ethanol production

INHIBITION OF THE GROWTH OF TOLERANT YEAST Saccharomyces cerevisiae STRAIN I136 BY A MIXTURE OF SYNTHETIC INHIBITORS

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